Abstract
Micropropagated shoots of wild cherry (Prunus avium L.) produced roots in auxin-free medium. Phloroglucinol (PG) increased the proportion of shoots that rooted, while phloretic acid reduced this response in medium with or without PG, and cancelled the promotive effect of PG. Concentration of PG also significantly affected rooting in media with and without auxin. The proportion of shoots rooting in media containing auxin, or auxin plus PG, increased with the number of successive subculture, but the proportion that rooted with PG alone was unaffected by the number of subcultures. Before the shoots had become responsive to auxin, 1 mM PG was more effective than auxin in inducing root formation.
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Baleriola-Lucas C and Mullins M (1984) Micropropagation of two French prune cultivars. Agronomie 4: 473–477
Hammatt N and Grant NJ (1993) Apparent rejuvenation of mature wild cherry (Prunus avium L.) during micropropagation. J Plant Physiol 141: 341–346
Hunter CS (1979) In vitro culture of Cinchona ledgeriana L. J Hort Sci 54: 111–114
Hunter LD (1975) Phloridzin and apple scab. Phytochemistry 14: 1519–1522
James DJ (1979) The role of auxins and phloroglucinol in adventitious root formation in Rubus and Fragaria grown in vitro. J Hort Sci 54: 273–277
James DJ and Thurbon IJ (1981) Phenolic compounds and other factors controlling rhizogenesis in vitro in the apple rootstocks M. 9 and M.26. Z Pflanzenphysiol 105: 11–20
James DJ and Thurbon IJ (1981) Shoot and root initiation in vitro in the apple rootstock M.9 and the promotive effects of phloroglucinol. J Hort Sci 56: 15–20
Jones OP (1976) Effect of phloridzin and phloroglucinol on apple shoots. Nature 262: 392–393, Erratum 262: 724
Jones OP (1983) In vitro propagation of tree crops. In: SH Mantell and H Smith, eds. Plant Biotechnology, Society for Experimental Biology, Seminar Series 18, pp 139–159. Cambridge University Press
Jones OP and Hatfield SGS (1976) Root initiation in apple shoots cultured in vitro with auxins and phenolic compounds. J Hort Sci 51: 495–499
Jones OP and Hopgood ME (1979) The successful propagation in vitro of two rootstocks of Prunus: the plum rootstock Pixy (P.insititia) and the cherry rootstock F12/1 (P. avium). J Hort Sci 54: 63–66
Murashige T and Skoog F (1962) A revised medium for the rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497
Passey AJ and Jones OP (1983) Shoot proliferation and rooting in vitro of Theobroma cacao L. type Amelonado. J Hort Sci 58: 589–592
Sriskandarajah S, Mullins MG and Nair Y (1982) Induction of adventitious rooting in vitro with difficult to propagate cultivars of apple. Plant Sci Lett 24: 1–9
Upton GJG (1992) Fisher's Exact Test. J R Statist Soc A 155: 395–402
Wang Q (1991) Factors affecting rooting of microcuttings of the pear rootstock BP10030. Scientia Hortic 45: 209–213
Webster CA and Jones OP (1989) Micropropagation of the apple rootstock M.9: effect of sustained subculture on apparent rejuvenation in vitro. J Hort Sci 64: 421–428
Wilson PJ and Van Staden J (1980) Rhizocaline, rooting cofactors, and the concept of promoters and inhibitors of adventitious rooting — a review. Ann Bot 66: 479–490
Zimmerman RH (1984) Rooting apple cultivars in vitro: Interactions among light, temperature, phloroglucinol and auxin. Plant Cell Tiss Org Cult 3: 301–311
Zimmerman RH and Broome O (1981) Phloroglucinol and in vitro rooting of apple cultivar cuttings. J Amer Soc Hort Sci 106: 648–652
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Hammatt, N. Promotion by phloroglucinol of adventitious root formation in micropropagated shoots of adult wild cherry (Prunus avium L.). Plant Growth Regul 14, 127–132 (1994). https://doi.org/10.1007/BF00025213
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DOI: https://doi.org/10.1007/BF00025213