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Structure and regulation of an ABA- and desiccation-responsive gene from the resurrection plant Craterostigma plantagineum

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Abstract

A gene from the resurrection plant Craterostigma plantagineum (CDeT6-19) encoding a protein with sequence similarity to a major group of late embryogenesis-abundant proteins (termed rab17, dehydrin or Lea D11) is regulated by abscisic acid (ABA) and desiccation. The corresponding transcript and protein is highly inducible in vegetative and callus tissue. To analyse the mechanism of CDeT6-19 regulation its promoter was fused to the β-glucuronidase reporter gene (GUS) and introduced by PEG (polyethylene glycol) into protoplasts of Craterostigma or tobacco. With 889 bp of promoter sequence the GUS expression was significantly stimulated by ABA treatment in transient expression assays. ABA responsiveness was still observed with shorter promoter fragments, although they gave rise to lower GUS activities. Sequence comparisons with promoters from related genes of other species identified the conservation of potential ABA-responsive elements. In tobacco and Craterostigma plants stably transformed with CDeT6-19 promoter constructs a basal GUS activity is observed. However, GUS expression is enhanced by ABA or drying treatment of leaf tissues. In tobacco high promoter activity was observed in mature seeds (embryos) and in pollen.

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References

  1. Bartels D, Hanke C, Schneider K, Michel D, Salamini F: A desiccation-related Elip-like gene from the resurrection plant Craterostigma plantagineum is regulated by light and ABA. EMBO J 11: 2771–2778 (1992).

    PubMed  Google Scholar 

  2. Bartels D, Schneider K, Piatkowski D, Elster R, Iturriaga G, Terstappen G, Le Tran Binh, Salamini F: Molecular analysis of desiccation tolerance in the resurrection plant Craterostigma plantagineum. In: Herrmann RG, Larkins B (eds) VI. NATO Advanced Study, pp. 663–671. Institute Plant Molecular Biology (1991).

  3. Bartels D, Schneider K, Terstappen G, Piatkowski D, Salamini F: Molecular cloning of abscisic acid modulated genes which are induced during desiccation of the resurrection plant Craterostigma plantagineum. Planta 181: 27–34 (1990).

    Article  Google Scholar 

  4. Bartels D, Velasco R, Schneider K, Forlani F, Furini A, Salamini F: Resurrection plants as model systems to study desiccation tolerance in higher plants. In: Mabry T, Nguyen H, Dixon R (eds) Applications and Prospects of Biotechnology for Arid and Semi-Arid Lands, in press.

  5. Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).

    PubMed  Google Scholar 

  6. Bradford MM: A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248–254 (1976).

    Article  PubMed  Google Scholar 

  7. Bray EA, Beachy RN: Regulation by ABA of a β-conglycinin expression cultured develo** soybean cotyledons. Plant Physiol 79: 746–750 (1985).

    Google Scholar 

  8. Bray EA: Regulation of gene expression by endogenous ABA during drought stress. In: Davies WJ, Jones HG (eds) Abscisic Acid Physiology and Biochemistry, pp. 81–98. Bios Scientific Publishers, Oxford, UK (1991).

    Google Scholar 

  9. Chen ZL, Pan NS, Beachy RN: A DNA sequence element that confers seed-specific enhancement to a constitutive promoter EMBO J 7: 297–302 (1988).

    Google Scholar 

  10. Close TJ, Kortt AA, Chandler PM: A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol 13: 95–108 (1989).

    PubMed  Google Scholar 

  11. Devereux J, Haeberli P, Smithies O: A comprehensive set of sequence analysis programs for the VAX. Nucl Acids Res 12: 387–395 (1984).

    PubMed  Google Scholar 

  12. DureIII L, Crouch M, Harada J, Ho TH, Mundy J, Quatrano R, Tomas T, Sung ZR: Common amino acid sequence domains among the Lea proteins of higher plants. Plant Mol Biol 12: 475–486 (1989).

    Google Scholar 

  13. Feinberg AP, Vogelstein B: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13 (1983).

    PubMed  Google Scholar 

  14. Finkelstein RR, Somerville CR: Three classes of abscisic acid (ABA)-insensitive mutations of Arabidopsis define genes that control overlap** subsets of ABA responses. Plant Physiol 94: 1172–1179 (1990).

    Google Scholar 

  15. Gaff DF: Desiccation tolerant flowering plants in Southern Africa. Science 174: 1033–1034 (1971).

    Google Scholar 

  16. Giraudat J, Hauge B, Valon C, Smalle J, Parcy F, Goodman H: Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4: 1251–1261 (1992).

    PubMed  Google Scholar 

  17. Guiltinan MJ, Marcotte WR, Quatrano RS: A plant leucine zipper protein that recognizes an abscisic acid response element. Science 250: 267–271 (1990).

    PubMed  Google Scholar 

  18. Henikoff S: Unidirectional digestion with exonuclease III created targeted breakpoints for DNA sequencing. Gene 28: 351–359 (1984).

    Article  PubMed  Google Scholar 

  19. Höfgen R, Willmitzer L: Storage of competent cells for Agrobacterium transformation. Nucl Acids Res 16: 9877 (1988).

    PubMed  Google Scholar 

  20. Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT: A simple and general method for transferring genes into plants. Science 227: 1227–1231 (1985).

    PubMed  Google Scholar 

  21. Jefferson RA, Kavanagh TA, Bevan MW: GUS fusion: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6: 3901–3907 (1987).

    PubMed  Google Scholar 

  22. Koncz C, Mayerhofer R, Koncz-Kalman Zs, Körber H, Rédei GP, Schell J: Isolation of a gene encoding a novel chloroplast protein by T-DNA tagging in Arabidopsis thaliana. EMBO J 9: 1337–1346 (1990).

    PubMed  Google Scholar 

  23. Koncz C, Schell J: The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimeric genes carried by a novel type of Agrobacterium binary vector. Mol Gen Genet 204: 383–396 (1986).

    Article  Google Scholar 

  24. Koornneef M, Reuling G, Karssen CM: The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana. Physiol Plant 61: 377–383 (1984).

    Google Scholar 

  25. Maliga P, Sz-Breznovitis A, Marton L: Streptomycin-resistant plants from callus culture of haploid tobacco. Nature 244: 29–30 (1973).

    Google Scholar 

  26. Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1982).

    Google Scholar 

  27. Marcotte WR, Russel SH, Quatrano R: Abscisic acid-responsive sequences from the Em gene of wheat. Plant Cell 1: 969–976 (1989).

    Article  PubMed  Google Scholar 

  28. McCarty DR, Hattori T, Carson CB, Vasil V, Lazar M, Vasil IK: The viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell 66: 895–905 (1991).

    Article  PubMed  Google Scholar 

  29. Michel D, Salamini F, Baga M, Dale P, Szalay A, Bartels D: Analysis of a desiccation and ABA-responsive promoter isolated from the resurrection plant Craterostigma plantagineum. Plant J 4: 29–40 (1993).

    Article  PubMed  Google Scholar 

  30. Mundy J, Chua NH: Abscisic acid and water-stress induce the expression of a novel rice gene. EMBO J 7: 2279–2286 (1988).

    PubMed  Google Scholar 

  31. Mundy J, Yamaguchi-Shinozaki K, Chua NH: Nuclear proteins bind conserved elements in the abscisic acid-responsive promoter of a rice RAB gene. Proc Natl Acad Sci USA 87: 1406–1410 (1990).

    PubMed  Google Scholar 

  32. Negrutiu I, Shilito R, Potrykus I, Biasini G, Sala F: hybrid genes in the analysis of transformation conditions. Plant Mol Biol 8: 363–373 (1987).

    Google Scholar 

  33. Oeda K, Salinos J, Chua NH: A tobacco bZIP transcription activator (TAF-1) binds to a G-box-like motif conserved in plant genes. EMBO J 10: 1793–1802 (1991).

    PubMed  Google Scholar 

  34. Ooms G, Hooykaas PJJ, vanVeen RJ, vanBeelen P, Reensburg-Tuink TJG, Schilperoort RA: Octopine Tiplasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. Plasmid 7: 15–29 (1982).

    PubMed  Google Scholar 

  35. Piatkowki D, Schneider K, Salamini F, Bartels D: Characterization of five abscisic acid-responsive cDNA clones from the desiccation-tolerant plant Craterostigma plantagineum and their relationship to other water-stress genes. Plant Physiol 94: 1682–1688 (1990).

    Google Scholar 

  36. Pla M, Vilardell J, Guiltinan MJ, Marcotte WR, Niogret MF, Quatrano RS, Pagès M: The cis-regulatory element CCACGTGG is involved in ABA and water stress responses of the maize gene rab 28. Plant Mol Biol 21: 259–266 (1993).

    PubMed  Google Scholar 

  37. Rogers JC, Rogers SW: Definition and functional implications of gibberellin and abscisic acid cis-acting hormone response complexes. Plant Cell 4: 1443–1451 (1992).

    Article  PubMed  Google Scholar 

  38. Schneider K, Wells B, Schmelzer E, Salamini F, Bartels D: Desiccation leads to the rapid accumulation of both cytosolic and chloroplastic proteins in the resurrection plant Craterostigma plantagineum Hochst. Planta 189: 120–131 (1993).

    Article  Google Scholar 

  39. Skriver K, Mundy J: Gene expression in response to abscisic acid and osmotic stress. Plant Cell 2: 503–512.

  40. Skriver K, Olsen FL, Rogers JC, Mundy J: Cis acting DNA elements responsive to gibberellin and its antagonist abscisic acid. Proc Natl Acad Sci USA 88: 7266–7270 (1991).

    PubMed  Google Scholar 

  41. Thomas TL, Vivekanada J, Bogue MA: ABA regulation of gene expression in embryos and mature plants. In: Davies WJ, Jones HG (eds) Abscisic Acid Physiology and Biochemistry, pp. 1125–1135. Bios Scientific Publishers, Oxford, UK (1991).

    Google Scholar 

  42. Uknes S, Dincher S, Friedrich L, Negrotto D, Williams S, Thompson-Taylor H, Potter S, Ward E, Ryals J: Regulation of pathogenesis-related protein-1a gene expression in tobacco. Plant Cell 5: 159–169 (1993).

    Article  PubMed  Google Scholar 

  43. Vilardell J, Mundy J, Stilling B, Leroux B, Pla M, Freyssinet G, Pagès M: Regulation of the maize rab17 gene promoter in transgenic heterologous systems. Plant Mol Biol 17: 985–993 (1991).

    PubMed  Google Scholar 

  44. Williams ME, Foster R, Chua NH: Sequences flanking the hexameric G-box core CACGTG affect the specificity of protein binding. Plant Cell 4: 485–496 (1992).

    Article  PubMed  Google Scholar 

  45. Yamaguchi-Shinozaki K, Mundy J, Chua NH: Four tightly linked rab genes are differentially expressed in rice. Plant Mol Biol 14: 29–39 (1989).

    Article  Google Scholar 

  46. Yamaguchi-Shinozaki K, Mino M, Mundy J, Chua NH: Analysis of an ABA-responsive rice gene promoter in transgenic tobacco. Plant Mol Biol 15: 905–912 (1990).

    PubMed  Google Scholar 

  47. Yamaguchi-Shinozaki K, Shinozaki K: The plant hormone abscisic acid mediates the drought-induced expression but not the seed-specific expression of rd22, a gene responsive to dehydration stress in Arabidopsis thaliana. Mol Gen Genet 238: 17–25 (1993).

    PubMed  Google Scholar 

  48. Yamaguchi-Shinozaki K, Shinozaki K: Characterization of the expression of a desiccation-responsive rd29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants. Mol Gen Genet 236: 331–340 (1993).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, D-50829, Köln, Germany

    Detlef Michel, Antonella Furini, Francesco Salamini & Dorothea Bartels

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  1. Detlef Michel
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  2. Antonella Furini
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  3. Francesco Salamini
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  4. Dorothea Bartels
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Michel, D., Furini, A., Salamini, F. et al. Structure and regulation of an ABA- and desiccation-responsive gene from the resurrection plant Craterostigma plantagineum . Plant Mol Biol 24, 549–560 (1994). https://doi.org/10.1007/BF00023553

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  • DOI: https://doi.org/10.1007/BF00023553

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