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Genes encoding phycobilisome linker polypeptides on the plastid genome of Aglaothamnion neglectum (Rhodophyta)

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Abstract

The genes encoding the phycobilisome anchor protein (apcE) and rod-core linker (cpcG) are on the plastid genome of the red alga Aglaothamnion neglectum. The apcE gene product is 5′ to and in the same operon as the α and β subunit genes of allophycocyanin. This arrangement is identical to the arrangement observed in many cyanobacteria. The cpcG gene product is 5′ to the operon encoding the α and β subunits of phycoerythrin, but is transcribed from the opposite DNA strand. This gene arrangement is different from that observed in cyanobacteria.

The amino acid sequences of the A. neglectum anchor protein and rod-core linker polypeptide, as deduced from the nucleotide sequences of the genes, are approximately 50% identical to analogous polypeptides from cyanobacteria and another eukaryotic alga Cyanophora paradoxa. The conserved nature of these proteins suggests that the structure of the core and the rod-core interface are very similar in phycobilisomes of cyanobacteria and eukaryotic red algae.

Environmental factors such as nutrient availability and light intensity can significantly affect the levels of mRNAs encoding the anchor protein and the rod-core linker polypeptide. Most of these changes are similar to the environmentally-controlled changes in the levels of phycobiliprotein transcripts of A. neglectum (Apt and Grossman 1992b). However, unlike the mRNAs encoding other phycobilisome components, the apcE transcript remains high during growth under conditions of nutrient deprivation.

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Abbreviations

AP:

allophycocyanin

PC:

phycocyanin

PE:

phycoerythrin

LCM :

core membrane linker (anchor protein)

LRC :

rod core linker

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Authors and Affiliations

  1. Department of Plant Biology, Carnegie Institution of Washington, 290 Panama Street, 94305, Stanford, CA, USA

    Kirk E. Apt & Arthur R. Grossman

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  1. Kirk E. Apt
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  2. Arthur R. Grossman
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Additional information

The nucleotide sequences reported in this paper have been deposited in the EMBL/Genbank data bases under the accession numbers Z11905, Z11907.

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Apt, K.E., Grossman, A.R. Genes encoding phycobilisome linker polypeptides on the plastid genome of Aglaothamnion neglectum (Rhodophyta). Photosynth Res 35, 235–245 (1993). https://doi.org/10.1007/BF00016555

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  • DOI: https://doi.org/10.1007/BF00016555

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