Abstract
Xylanase is a well-known enzyme for the conversion of agricultural biomass into xylose using enzymatic hydrolysis process. Agricultural residues are the rich source of lignocellulosic biomass. Recently there has been increasing interest in the use of agricultural residues for the production of industrially important enzymes along with other value-added by-products. Large-scale purification of the desired enzyme from fermentation broth and purification of enzymes via economically feasible methods are the main obstacle in biotechnological industries. In the present study, aqueous two-phase system (ATPS) was utilized for the purification of extracellular crude xylanase obtained from the submerged culture of Aspergillus oryzae LC1 (ITCC-8571/NAIMCC-F-03390). Various sulphate salts were tested for xylanase purification, among them MgSO4 showed best results with PEG and was selected for further studies. Response surface methodology based on Box-Behnken design was used for optimizing the ATPS parameters, such as the molecular weight of PEG, MgSO4 and PEG concentration. The purification factor of xylanase was found maximum in the presence of high-molecular-weight PEG (8000), PEG intermediate concentration (11.3% w/w) and high MgSO4 salt concentration (22.5% w/w). A 13-fold increase in purification factor was obtained under optimized conditions, with partition coefficient 8.8% and enzyme yield 86.8% at the top phase. The partially purified xylanase obtained using ATPS was further used for the enzymatic hydrolysis of different agricultural residues, followed by the analysis of the hydrolysis products by TLC.
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Acknowledgement
The authors are thankful to DBT for providing the financial support (Grant No. BT/304/NE/TBP/2012).
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Bhardwaj, N., Verma, P. (2020). Extraction of Fungal Xylanase Using ATPS-PEG/Sulphate and Its Application in Hydrolysis of Agricultural Residues. In: Sadhukhan, P., Premi, S. (eds) Biotechnological Applications in Human Health. Springer, Singapore. https://doi.org/10.1007/978-981-15-3453-9_11
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DOI: https://doi.org/10.1007/978-981-15-3453-9_11
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