Abstract
The strain under study, Alcaligenes faecalis A15, is a diazotroph isolated from the (endo)rhizosphere of paddy rice. To study the interaction of Alcaligenes faecalis A15 with rice we have applied the gus A reporter-gene technology. With a fusion constitutively expressing the gus A gene we have analysed the colonization patterns of rice roots and the environmental factors that affect colonization. With a transcriptional fusion of the Alcaligenes faecalis A15 nifH gene and gusA we were able to visualize the expression of the nitrogen fixation genes, both in planta and in free-living conditions. Finally, we have compared nifH expression in Azospirillum brasÃlense, Azospirillum irakense, and Alcaligenes faecalis A15 under plant root-associated conditions.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Baldini V L D, Alvarez M A, Baldini J I, Döbereiner J 1986. Establishment of inoculated Azospirillum spp in the rhizosphere and in roots of field grown wheat and sorghum. Plant Soil, 90, 35–46.
Bender G L, Preston L, Barnard D, Rolfe B G 1990. Formation of nodule-like structures on the roots of the non-legumes rice and wheat. In: Gresshoff P M, Roth L E, Stacey G, Newton W E, eds. Nitrogen Fixation: Achievements and Objectives. Chapman & Hall, London, 825.
Chan Y K 1985. Denitrification by a diazotrophic Pseudomonas species. Can. J. Microbiol., 31, 1136–1141.
Cocking E C, Al-Mallah M K, Benson E, Davey M R 1990. Nodulation of non-legumes by rhizobia. In: Gresshoff P M, Roth L E, Stacey G, Newton W E, eds. Nitrogen Fixation: Achievements and Objectives. Chapman & Hall, London, 813–823.
deBruijn F J, **g Y, Dazzo F B 1995. Potential and pitfalls of trying to extend symbiotic interactions of nitrogen-fixing organisms to presently non-nodulated plants, such as rice. Plant Soil, 174, 225–240.
Döbereiner J, Reis V M, Paula M A, Olivares F 1993. Endophytic diazotrophs in sugar cane, cereals and tuber plants. In: Palacios J, Mora J, Newton W E, eds. New Horizons in Nitrogen Fixation. Proceedings of the 9th International Symposium on Nitrogen Fixation. Kluwer, Dordrecht, 671–676.
Figurski D H, Helinski D R 1979. Replication of an origin containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA, 76, 1648–1652.
Gallagher S R (ed.) 1992. GUS Protocols: using the GUS gene as a reporter of gene expression. Academic Press, London.
Gough C, Vasse J, Galera C, Webster G, Cocking E, Denarie J 1997. Interactions between bacterial diazotrophs and non-legume dicots: Arabidopsis thaliana as a model plant. Plant Soil, 194, 123–130.
Haahtela K, Helander I, Nurmiaho-Lassila E L, Sundman V 1983. Morphological and physiological characteristics and lypopolysaccharide composition of nitrogen-fixing (C2H2 reducing) root-associated Pseudomonas sp. Can. J. Microbiol., 29, 874–880.
Jefferson R A 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep., 5, 387–405.
**g Y, Li G, ** G, Shan X, Zhang B, Guang C, Li J 1990. Rice root nodules with acetylene reduction activity. In: Gresshoff P M, Roth L E, Stacey G, Newton W E, eds. Nitrogen fixation: achievements and objectives. Chapman & Hall, London, 829.
Khammas K M, Ageron E, Grimont PAD, Kaiser P 1989. Azospirillum irakense sp. nov. a nitrogen fixing bacterium associated with rice roots and rhizosphere soil. Res. Microbiol., 140, 679–693.
Maniatis T, Fritsch E F, Sambrook J 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor, New York.
Miller J H 1972. Experiments in Molecular Genetics. Cold Spring Harbor, New York.
Qiu Y, Zhou S, Mo X, Wang D, Hong J 1991. Study of nitrogen fixing bacteria associated with rice root. Isolation and identification of organisms. In: You C B (ed.) The Associative Nitrogen Fixation in the Rice Rhizosphere. Agricultural Publishing House, Bei**g, China, 13–18.
Reinhold-Hurek B, Hurek T, Gillis M, Hoste B, Vancanneyt M, Kersters K, DeLey J 1993. Azoarcus gen. nov., nitrogen fixing proteobacteria associated with the roots of Kallar grass (Leptochloa fusca (L.) Kunth), and description of two species, Azoarcus indigens sp. nov. and Azoarcus communis sp. nov. Int. J. Syst. Bacteriol., 43, 574–584.
Vande Broek A, Michiels J, Van Gool A, Vanderleyden J 1993. Spatial-temporal colonization patterns of Azospirillum brasilense on the wheat root surface and expression of the bacterial nifH gene during association. Mol. Plant Microb. Interact, 6, 592–600.
Vanstockem M, Michiels K, Vanderleyden J, Van Gool A 1987. Transposon mutagenesis of Azospirillum brasilense and Azospirillum lipoferum, physical analysis of Tn5 and Tn5-mob insertion mutants. Appl. Environ. Microbiol., 53, 410–415.
Watanabe I, So R, Ladha J K, Katajama-Fujimura Y, Kuraishi H 1987. A new nitrogen-fixing species of pseudomonad: Pseudomonas diazotrophicus sp. nov. isolated from the root of wetland rice. Can. J. Microbiol., 33, 670–678.
Yoshida S, Formo D A, Cock J H 1971. Laboratory Manual for Physiological Studies of Rice. IRRI, Los Banos, Philippines.
You C B, Song W, Wang H X, Li J P, Lin M, Hai W L 1991. Association of Alcaligenes faecalis with wetland rice. Plant Soil, 137, 81–85.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Vermeiren, H., Vanderleyden, J., Hai, W. (1998). Colonization and nifH expression on rice roots by Alcaligenes faecalis A15. In: Malik, K.A., Mirza, M.S., Ladha, J.K. (eds) Nitrogen Fixation with Non-Legumes. Developments in Plant and Soil Sciences, vol 79. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-5232-7_20
Download citation
DOI: https://doi.org/10.1007/978-94-011-5232-7_20
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-6202-2
Online ISBN: 978-94-011-5232-7
eBook Packages: Springer Book Archive