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Functional Genomics in the Whitefly Bemisia tabaci Species Complex

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Management of Insect Pests to Agriculture

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Abstract

Whiteflies (Hemiptera: Aleyrodidae) include some important pests of agricultural and horticultural crops in temperate and tropical regions. The two most damaging and widespread species are the tobacco or cotton whitefly (Bemisia tabaci) and the greenhouse whitefly (Trialeurodes vaporariorum). Bemisia tabaci is now recognized as a species complex of at least 35 cryptic species. During the past 20 years, two species of the complex, Middle East-Asia Minor 1 (hereafter MEAM1) and Mediterranean (hereafter MED), which have been commonly referred to as the B and Q ‘biotype’, have risen to international prominence due to their global invasion. Despite their global prevalence, genomic sequence resources available for the whiteflies were scarce until recent years. Hence deciphering the functional genomics of whiteflies has become crucial to advance studies on their biology. The recent advancement in genomic technologies offers great opportunities for a better understanding of the complex mechanisms underlying whitefly problems. With the development of high performance sequencing technology, transcript profiling techniques allow the simultaneous examination of thousands of genes, and can be utilized to study changes in gene expression. In this review, we will summarize the recent developments in whitefly transcriptomes and gene expression profiling during virus transmission, whitefly-plant interactions and stress responses. These research efforts have provided valuable datasets for future investigations on the molecular mechanisms of whitefly biology and are expected to open important avenues into the discovery of novel strategies for whitefly management.

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References

  • Alon, M., Elbaz, M., Ben-Zvi, M. M., Feldmesser, E., Vainstein, A., & Morin, S. (2012). Insights into the transcriptomics of polyphagy: Bemisia tabaci adaptability to phenylpropanoids involves coordinated expression of defense and metabolic genes. Insect Biochemistry and Molecular Biology, 42, 251–263.

    Article  CAS  PubMed  Google Scholar 

  • Boykin, L. M., & De Barro, P. J. (2014). A practical guide to identifying members of the Bemisia tabaci species complex and other morphologically identical species. Frontiers in Ecology and Evolution, 2, 45. doi:10.3389/fevo.2014.00045.

    Article  Google Scholar 

  • Byrne, D. N., & Bellows, T. S. (1991). Whitefly biology. Annual Review of Entomology, 36, 431–457.

    Article  Google Scholar 

  • Crowder, D. W., Dennehy, T. J., Ellers-Kirk, C., Yafuso, L. C., Ellsworth, P. C., Tabashnik, B. E., & Carriere, Y. (2007). Field evaluation of resistance to pyriproxyfen in Bemisia tabaci (B biotype). Journal of Economic Entomology, 100, 1650–1656.

    Article  CAS  PubMed  Google Scholar 

  • Czosnek, H., & Brown, J. K. (2010). The whitefly genome–white paper: A proposal to sequence multiple genomes of Bemisia tabaci. In P. A. Stansly & S. E. Naranjo (Eds.), Bemisia: Bionomics and management of a global pest (pp. 503–532). Dordrecht/London: Springer.

    Google Scholar 

  • Czosnek, H., & Ghanim, M. (2002). The circulative pathway of begomoviruses in the whitefly vector Bemisia tabaci – insights from studies with Tomato yellow leaf curl virus. The Annals of Applied Biology, 140, 215–231.

    Article  Google Scholar 

  • Dalton, R. (2006). Whitefly infestations: The Christmas Invasion. Nature, 443, 898–900.

    Article  CAS  PubMed  Google Scholar 

  • De Barro, P. J., Liu, S.-S., Boykin, L. M., & Dinsdale, A. B. (2011). Bemisia tabaci: A statement of species status. Annual Review of Entomology, 56, 1–19.

    Article  PubMed  Google Scholar 

  • Dennehy, T. J., Degain, B. A., Harpold, V. S., Zaborac, M., Morin, S., Fabrick, J. A., Nichols, R. L., Brown, J. K., Byrne, F. J., & Li, X. (2010). Extraordinary resistance to insecticides reveals exotic Q biotype of Bemisia tabaci in the New World. Journal of Economic Entomology, 103, 2174–2186.

    Article  CAS  PubMed  Google Scholar 

  • Dubey, N. K., Goel, R., Ranjan, A., Idris, A., Singh, S. K., Bag, S. K., Chandrashekar, K., Pandey, K. D., Singh, P. K., & Sawant, S. V. (2013). Comparative transcriptome analysis of Gossypium hirsutum L. in response to sap sucking insects: Aphid and whitefly. BMC Genomics, 14, 241.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Edwards, O., & Papanicolaou, A. (2012). A roadmap for whitefly genomics research: Lessons from previous insect genome projects. Journal of Integrative Agriculture, 11, 269–280.

    Article  CAS  Google Scholar 

  • Elbaz, M., Halon, E., Malka, O., Malitsky, S., Blum, E., Aharoni, A., & Morin, S. (2012). Asymmetric adaptation to indolic and aliphatic glucosinolates in the B and Q sibling species of Bemisia tabaci (Hemiptera: Aleyrodidae). Molecular Ecology, 21, 4533–4546.

    Article  CAS  PubMed  Google Scholar 

  • Estrada-Hernandez, M. G., Valenzuela-Soto, J. H., Ibarra-Laclette, E., & Delano-Frier, J. P. (2009). Differential gene expression in whitefly Bemisia tabaci-infested tomato (Solanum lycopersicum) plants at progressing developmental stages of the insect's life cycle. Physiologia Plantarum, 137, 44–60.

    Article  CAS  PubMed  Google Scholar 

  • Ghanim, M., & Kontsedalov, S. (2007). Gene expression in pyriproxyfen-resistant Bemisia tabaci Q biotype. Pest Management Science, 63, 776–783.

    Article  CAS  PubMed  Google Scholar 

  • Ghanim, M., Morin, S., & Czosnek, H. (2001a). Rate of Tomato yellow leaf curl virus translocation in the circulative transmission pathway of its vector, the whitefly Bemisia tabaci. Phytopathology, 91, 188–196.

    Article  CAS  PubMed  Google Scholar 

  • Ghanim, M., Rosell, R. C., Campbell, L. R., Czosnek, H., Brown, J. K., & Ullman, D. E. (2001b). Digestive, salivary, and reproductive organs of Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) B type. Journal of Morphology, 248, 22–40.

    Article  CAS  PubMed  Google Scholar 

  • Gibbons, J. G., Janson, E. M., Hittinger, C. T., Johnston, M., Abbot, P., & Rokas, A. (2009). Benchmarking next-generation transcriptome sequencing for functional and evolutionary genomics. Molecular Biology and Evolution, 26, 2731–2744.

    Article  CAS  PubMed  Google Scholar 

  • Gorman, K., Slater, R., Blande, J. D., Clarke, A., Wren, J., McCaffery, A., & Denholm, I. (2010). Cross-resistance relationships between neonicotinoids and pymetrozine in Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Management Science, 66, 1186–1190.

    Article  CAS  PubMed  Google Scholar 

  • Götz, M., Popovski, S., Kollenberg, M., Gorovits, R., Brown, J. K., Cicero, J. M., Czosnek, H., Winter, S., & Ghanim, M. (2012). Implication of Bemisia tabaci heat shock protein 70 in begomovirus-whitefly interactions. Journal of Virology, 86, 13241–13252.

    Article  PubMed  PubMed Central  Google Scholar 

  • Harrison, B. (1985). Advances in geminivirus research. Annual Review of Phytopathology, 23, 55–82.

    Article  CAS  Google Scholar 

  • Hogenhout, S. A., Ammar, E. D., Whitfield, A. E., & Redinbaugh, M. G. (2008). Insect vector interactions with persistently transmitted viruses. Annual Review of Phytopathology, 46, 327–359.

    Article  CAS  PubMed  Google Scholar 

  • Horowitz, A., Antignus, Y., & Gerling, D. (2011). Management of Bemisia tabaci whiteflies. Dordrecht: Springer.

    Book  Google Scholar 

  • Jiu, M., Zhou, X. P., & Liu, S. S. (2006). Acquisition and transmission of two begomoviruses by the B and a non – B biotype of Bemisia tabaci from Zhejiang. Journal of Phytopathology, 154, 587–591.

    Article  CAS  Google Scholar 

  • Karatolos, N., Pauchet, Y., Wilkinson, P., Chauhan, R., Denholm, I., Gorman, K., Nelson, D. R., Bass, C., & Williamson, M. S. (2011). Pyrosequencing the transcriptome of the greenhouse whitefly, Trialeurodes vaporariorum reveals multiple transcripts encoding insecticide targets and detoxifying enzymes. BMC Genomics, 12, 56.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kempema, L. A., Cui, X., Holzer, F. M., & Walling, L. L. (2007). Arabidopsis transcriptome changes in response to phloem-feeding silverleaf whitefly nymphs. Similarities and distinctions in responses to aphids. Plant Physiology, 143, 849–865.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Legg, J. P., Shirima, R., Tajebe, L. S., Guastella, D., Boniface, S., Jeremiah, S., Nsami, E., Chikoti, P., & Rapisarda, C. (2014). Biology and management of Bemisia whitefly vectors of cassava virus pandemics in Africa. Pest Management Science, 70, 1446–1453.

    Article  CAS  PubMed  Google Scholar 

  • Leshkowitz, D., Gazit, S., Reuveni, E., Ghanim, M., Czosnek, H., McKenzie, C., Shatters, R. L., & Brown, J. K. (2006). Whitefly (Bemisia tabaci) genome project: Analysis of sequenced clones from egg, instar, and adult (viruliferous and non-viruliferous) cDNA libraries. BMC Genomics, 7, 79.

    Article  PubMed  PubMed Central  Google Scholar 

  • Li, J. M., Ruan, Y. M., Li, F. F., Liu, S. S., & Wang, X. W. (2011). Gene expression profiling of the whitefly (Bemisia tabaci) Middle East – Asia Minor 1 feeding on healthy and Tomato yellow leaf curl China virus-infected tobacco. Instructional Science, 18, 11–22.

    Google Scholar 

  • Liu, S. S., De Barro, P. J., Xu, J., Luan, J. B., Zang, L. S., Ruan, Y. M., & Wan, F. H. (2007). Asymmetric mating interactions drive widespread invasion and displacement in a whitefly. Science, 318, 1769–1772.

    Article  CAS  PubMed  Google Scholar 

  • Liu, S. S., Colvin, J., & De Barro, P. (2012). Species concepts as applied to the whitefly Bemisia tabaci systematics: How many species are there? Journal of Integrative Agriculture, 11, 176–186.

    Article  Google Scholar 

  • Lu, Z. C., & Wan, F. H. (2008). Differential gene expression in whitefly (Bemisia tabaci) B-biotype females and males under heat-shock condition. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 3, 257–262.

    Google Scholar 

  • Luan, J. B., Li, J. M., Varela, N., Wang, Y. L., Li, F. F., Bao, Y. Y., Zhang, C. X., Liu, S. S., & Wang, X. W. (2011). Global analysis of the transcriptional response of whitefly to Tomato yellow leaf curl China virus reveals the relationship of coevolved adaptations. Journal of Virology, 85, 3330–3340.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Luan, J. B., Wang, Y. L., Wang, J., Wang, X. W., & Liu, S. S. (2013a). Detoxification activity and energy cost is attenuated in whiteflies feeding on Tomato yellow leaf curl China virus-infected tobacco plants. Insect Molecular Biology, 22, 597–607.

    Article  CAS  PubMed  Google Scholar 

  • Luan, J. B., Yao, D. M., Zhang, T., Walling, L. L., Yang, M., Wang, Y. J., & Liu, S. S. (2013b). Suppression of terpenoid synthesis in plants by a virus promotes its mutualism with vectors. Ecology Letters, 16, 390–398.

    Article  PubMed  Google Scholar 

  • Mahadav, A., Gerling, D., Gottlieb, Y., Czosnek, H., & Ghanim, M. (2008). Parasitization by the wasp Eretmocerus mundus induces transcription of genes related to immune response and symbiotic bacteria proliferation in the whitefly Bemisia tabaci. BMC Genomics, 9, 342.

    Article  PubMed  PubMed Central  Google Scholar 

  • Mahadav, A., Kontsedalov, S., Czosnek, H., & Ghanim, M. (2009). Thermotolerance and gene expression following heat stress in the whitefly Bemisia tabaci B and Q biotypes. Insect Biochemistry and Molecular Biology, 39, 668–676.

    Article  CAS  PubMed  Google Scholar 

  • McKenzie, C. L., Sisisterra, X. H., Powell, C. A., Bausher, M., Albano, J. P., & Shatters, R. G., Jr. (2005). Deciphering changes in plant physiological response to whitefly feeding using microarray technology. Acta Horticulturae, 695, 347–352.

    Article  CAS  Google Scholar 

  • Moriones, E., & Navas-Castillo, J. (2000). Tomato yellow leaf curl virus, an emerging virus complex causing epidemics worldwide. Virus Research, 71, 123–134.

    Article  CAS  PubMed  Google Scholar 

  • Mound, L. A., & Halsey, S. H. (1978). Whitefly of the world. New York: Wiley.

    Google Scholar 

  • Naranjo, S. E., Castle, S. J., De Barro, P. J., & Liu, S. S. (2010). Population dynamics, demography, dispersal and spread of Bemisia tabaci. In P. A. Stansly & S. E. Naranjo (Eds.), Bemisia: Bionomics and management of a global pest (pp. 185–226). Dordrecht/London: Springer.

    Google Scholar 

  • Navas-Castillo, J., Fiallo-Olivé, E., & Sánchez-Campos, S. (2011). Emerging virus diseases transmitted by whiteflies. Annual Review of Physical Chemistry, 49, 219–248.

    CAS  Google Scholar 

  • Puthoff, D. P., Holzer, F. M., Perring, T. M., & Walling, L. L. (2010). Tomato pathogenesis-related protein genes are expressed in response to Trialeurodes vaporariorum and Bemisia tabaci biotype B feeding. Journal of Chemical Ecology, 36, 1271–1285.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Seal, S., Patel, M., Collins, C., Colvin, J., & Bailey, D. (2012). Next generation transcriptome sequencing and quantitative real-time PCR technologies for characterisation of the Bemisia tabaci Asia 1 mtCOI phylogenetic clade. Journal of Integrative Agriculture, 11, 281–292.

    Article  CAS  Google Scholar 

  • Su, Y. L., Li, J. M., Li, M., Luan, J. B., Ye, X. D., Wang, X. W., & Liu, S. S. (2012). Transcriptomic analysis of the salivary glands of an invasive whitefly. PLoS One, 7, e39303.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tang, F., Lao, K., & Surani, M. A. (2011). Development and applications of single-cell transcriptome analysis. Nature Methods, 8, S6–S11.

    CAS  PubMed  PubMed Central  Google Scholar 

  • van de Ven, W. T., LeVesque, C. S., Perring, T. M., & Walling, L. L. (2000). Local and systemic changes in squash gene expression in response to silverleaf whitefly feeding. The Plant Cell, 12, 1409–1423.

    Article  PubMed  PubMed Central  Google Scholar 

  • Varma, A., Mandal, B., & Singh, M. K. (2011). Global emergence and spread of whitefly (Bemisia tabaci) transmitted geminiviruses. In W.M.O. Thompson (Ed.), The whitefly, Bemisia tabaci (Homoptera: Aleyrodidae) interaction with geminivirus-infected host plants (pp. 205–292). Dordrecht: Springer.

    Google Scholar 

  • Wang, Z., Yao, M., & Wu, Y. (2009). Cross-resistance, inheritance and biochemical mechanisms of imidacloprid resistance in B-biotype Bemisia tabaci. Pest Management Science, 65, 1189–1194.

    Article  CAS  PubMed  Google Scholar 

  • Wang, X. W., Luan, J. B., Li, J. M., Bao, Y. Y., Zhang, C. X., & Liu, S. S. (2010). De novo characterization of a whitefly transcriptome and analysis of its gene expression during development. BMC Genomics, 11, 400.

    Article  PubMed  PubMed Central  Google Scholar 

  • Wang, X. W., Luan, J. B., Li, J. M., Su, Y. L., **a, J., & Liu, S. S. (2011). Transcriptome analysis and comparison reveal divergence between two invasive whitefly cryptic species. BMC Genomics, 12, 458.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang, X. W., Zhao, Q. Y., Luan, J. B., Wang, Y. J., Yan, G. H., & Liu, S. S. (2012). Analysis of a native whitefly transcriptome and its sequence divergence with two invasive whitefly species. BMC Genomics, 13, 529.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang, Y. L., Wang, Y. J., Luan, J. B., Yan, G. H., Liu, S. S., & Wang, X. W. (2013). Analysis of the transcriptional differences between indigenous and invasive whiteflies reveals possible mechanisms of whitefly invasion. PLoS One, 8, e62176.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • **a, J., Zhang, C. R., Zhang, S., Li, F. F., Feng, M. G., Wang, X. W., & Liu, S. S. (2013). Analysis of whitefly transcriptional responses to Beauveria bassiana infection reveals new insights into insect-fungus interactions. PLoS One, 8, e68185.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • **e, W., Meng, Q., Wu, Q., Wang, S., Yang, X., Yang, N., Li, R., Jiao, X., Pan, H., & Liu, B. (2012a). Pyrosequencing the Bemisia tabaci transcriptome reveals a highly diverse bacterial community and a robust system for insecticide resistance. PLoS One, 7, e35181.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • **e, W., Yang, X., Wang, S. I., Wu, Q. J., Yang, N. N., Li, R. M., Jiao, X. G., Pan, H. P., Liu, B. M., Feng, Y. T., Xu, B. Y., Zhou, X. G., & Zhang, Y. J. (2012b). Gene expression profiling in the thiamethoxam resistant and susceptible B-biotype sweetpotato whitefly, Bemisia tabaci. Journal of Insect Science, 12, 46.

    Article  PubMed  PubMed Central  Google Scholar 

  • **e, W., Guo, L., Jiao, X., Yang, N., Yang, X., Wu, Q., Wang, S., Zhou, X., & Zhang, Y. (2014). Transcriptomic dissection of sexual differences in Bemisia tabaci, an invasive agricultural pest worldwide. Scientific Reports, 4, 4088.

    Article  PubMed  PubMed Central  Google Scholar 

  • Xu, J., De Barro, P. J., & Liu, S. S. (2010). Reproductive incompatibility among genetic groups of Bemisia tabaci supports the proposition that the whitefly is a cryptic species complex. Bulletin of Entomological Research, 100, 359–366.

    Article  CAS  PubMed  Google Scholar 

  • Yang, N., **e, W., Jones, C. M., Bass, C., Jiao, X., Yang, X., Liu, B., Li, R., & Zhang, Y. (2013a). Transcriptome profiling of the whitefly Bemisia tabaci reveals stage-specific gene expression signatures for thiamethoxam resistance. Insect Molecular Biology, 22, 485–496.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yang, N., **e, W., Yang, X., Wang, S., Wu, Q., Li, R., Pan, H., Liu, B., Shi, X., Fang, Y., Xu, B., Zhou, X., & Zhang, Y. (2013b). Transcriptomic and proteomic responses of sweetpotato whitefly, Bemisia tabaci, to thiamethoxam. PLoS One, 8, e61820.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ye, X. D., Su, Y. L., Zhao, Q. Y., **a, W. Q., Liu, S. S., & Wang, X. W. (2014). Transcriptomic analyses reveal the adaptive features and biological differences of guts from two invasive whitefly species. BMC Genomics, 15, 370.

    Article  PubMed  PubMed Central  Google Scholar 

  • Zarate, S. I., Kempema, L. A., & Walling, L. L. (2007). Silverleaf whitefly induces salicylic acid defenses and suppresses effectual jasmonic acid defenses. Plant Physiology, 143, 866–875.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang, T., Luan, J. B., Qi, J. F., Huang, C. J., Li, M., Zhou, X. P., & Liu, S. S. (2012). Begomovirus- whitefly mutualism is achieved through repression of plant defences by a virus pathogenicity factor. Molecular Ecology, 21, 1294–1304.

    Article  PubMed  Google Scholar 

  • Zhang, C. R., Zhang, S., **a, J., Li, F. F., **a, W. Q., Liu, S. S., & Wang, X. W. (2014). The immune strategy and stress response of the Mediterranean species of the Bemisia tabaci complex to an orally delivered bacterial pathogen. PLoS One, 9, e94477.

    Article  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou, 310058, China

    **ao-Wei Wang & Shu-Sheng Liu

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  1. **ao-Wei Wang
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Correspondence to **ao-Wei Wang or Shu-Sheng Liu .

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Editors and Affiliations

  1. Institute of Plant Sciences and Genetics in Agriculture Robert H. Smith Faculty of Agriculture Food and Environment, The Hebrew University of Jerusalem, Rehovot, Israel

    Henryk Czosnek

  2. Department of Entomology, Volcani Center, Bet Dagan, Israel

    Murad Ghanim

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Wang, XW., Liu, SS. (2016). Functional Genomics in the Whitefly Bemisia tabaci Species Complex. In: Czosnek, H., Ghanim, M. (eds) Management of Insect Pests to Agriculture. Springer, Cham. https://doi.org/10.1007/978-3-319-24049-7_8

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