Abstract
We present two different methods for determining levels of glutathione in complex biological samples and plasma. The DTNB/GR enzyme recycling method is sensitive and requires no specialized equipment. The HPLC method is particularly useful for situations in which sample amounts are limited. Detailed instructions for performing each method as well as the advantages and disadvantages of each are discussed in this chapter.
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Acknowledgments
The authors would like to thank Kathryn Heyob for her assistance in the preparation of this chapter. This work was supported by 5K08HL093365-02 (T.E.T.).
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Tipple, T.E., Rogers, L.K. (2012). Methods for the Determination of Plasma or Tissue Glutathione Levels. In: Harris, C., Hansen, J. (eds) Developmental Toxicology. Methods in Molecular Biology, vol 889. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-867-2_20
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DOI: https://doi.org/10.1007/978-1-61779-867-2_20
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