Growth Measurements: Estimation of Cell Division and Cell Expansion

  • Protocol
  • First Online:
Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 877))

Abstract

The main parameters for the estimation of growth within in vitro cultures are reviewed. Procedures to measure these parameters are described, emphasizing in each case their convenience of use, depending on the features of the culture evaluated.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
EUR 44.95
Price includes VAT (Germany)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
EUR 96.29
Price includes VAT (Germany)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
EUR 128.39
Price includes VAT (Germany)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info
Hardcover Book
EUR 181.89
Price includes VAT (Germany)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free ship** worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Ryu DDY, Lee SO, Romani RJ (1990) Determi­nation of growth rate for plant cell cultures: com­parative studies. Biotechnol Bioeng 35:305–311

    Article  PubMed  CAS  Google Scholar 

  2. Fowler MW (1987) Products from plant cells. In: Bu’lok J, Kristiansen B (eds) Basic biotechnology. Academic, London, pp 525–544

    Google Scholar 

  3. Su WW (1995) Bioprocessing technology for plant cell suspension cultures. Appl Biochem Biotechnol 50:189–230

    Article  CAS  Google Scholar 

  4. Fowler MW (1982) The large scale cultivation of plant cells. In: Bull MJ (ed) Progress in industrial microbiology, vol 16. Elsevier, Amsterdam, pp 207–229

    Google Scholar 

  5. González RC, Woods RE, Eddins SL (2002) Digital image processing using Matlab. Prentice Hall, London

    Google Scholar 

  6. Trejo-Tapia G, Arias-Castro C, Rodríguez-Mendiola M (2003) Influence of the culture medium constituents and inoculum size on the accumulation of blue pigment and cell growth of Lavandula spica. Plant Cell Tissue Organ Cult 72:7–12

    Article  CAS  Google Scholar 

  7. King PJ, Street HE (1977) Growth patterns in cell cultures. In: Street HE (ed) Botanical monographs, vol 11, Plant tissue and cell culture. Blackwell, Oxford

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Gregorio Godoy-Hernández .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2012 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Godoy-Hernández, G., Vázquez-Flota, F.A. (2012). Growth Measurements: Estimation of Cell Division and Cell Expansion. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 877. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-818-4_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-61779-818-4_4

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-817-7

  • Online ISBN: 978-1-61779-818-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Navigation