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Intranasal Delivery of Neuropeptides

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Neuropeptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 789))

Abstract

A major barrier to entry of neuropeptides into the brain is low bioavailability and presence of the blood–brain barrier. Intranasal delivery of neuropeptides provides a potentially promising alternative to other routes of administration, since a direct pathway exists between the olfactory neuroepithelium and the brain. Use of the rat as an animal model in nose to brain delivery of neuropeptides allows for several advantages, including a large surface area within the nasal cavity dedicated to olfactory epithelium and robust neuronal pathways extending to and from most areas of the brain from the nose via the olfactory cortex. A major disadvantage to using rats for nose to brain delivery is the difficulty in selectively targeting the posterior olfactory epithelium (which facilitates delivery to the brain) over the more anterior respiratory epithelium (which facilitates delivery to the lungs and secondarily to the peripheral blood) in the nasal cavity. We have developed a novel delivery system that consists of surgically implanting stainless-steel cannulas in the dorsal aspect of the nasal cavity overlying the olfactory neuroepithelium, thereby allowing neuropeptide compounds to bypass the respiratory epithelium.

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Author information

Authors and Affiliations

  1. Program in Medical Neuroscience, Indiana University School of Medicine, Indianapolis, IN, USA

    Michael C. Veronesi

  2. Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN, USA

    Daniel J. Kubek

  3. Department of Anatomy and Cell Biology and Program in Medical Neuroscience, Indiana University School of Medicine, Indianapolis, IN, USA

    Michael J. Kubek

Authors
  1. Michael C. Veronesi
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  2. Daniel J. Kubek
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  3. Michael J. Kubek
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Correspondence to Michael J. Kubek .

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Editors and Affiliations

  1. , Dipt. di Morfofisiologia Veterinaria, UniversitĂ  degli Studi di Torino, Via Leonardo da Vinci 44, Grugliasco, 10095, Torino, Italy

    Adalberto Merighi

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Veronesi, M.C., Kubek, D.J., Kubek, M.J. (2011). Intranasal Delivery of Neuropeptides. In: Merighi, A. (eds) Neuropeptides. Methods in Molecular Biology, vol 789. Humana Press. https://doi.org/10.1007/978-1-61779-310-3_20

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  • DOI: https://doi.org/10.1007/978-1-61779-310-3_20

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-61779-309-7

  • Online ISBN: 978-1-61779-310-3

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