Abstract
This chapter will describe the most common immunocytochemical method utilized in the stem cell field – using fluorescently tagged secondary antibodies to detect a primary antibody that is bound to an epitope on a molecule of interest. Secondary antibodies recognize the heavy chain of the primary antibody’s isotype. Generally, these methods employ an incubation period of the sample with the primary antibody, a series of washes to remove unbound primary antibody, a secondary incubation period of the sample with the fluorescently conjugated secondary antibody, followed by washes and preparation for microscopy.
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Acknowledgments
We gratefully acknowledge intellectual contributions from Lisa A. Flanagan, Richard Pepple, Boback Ziaeian, and Theo Palmer.
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Nethercott, H.E., Brick, D.J., Schwartz, P.H. (2011). Immunocytochemical Analysis of Human Pluripotent Stem Cells. In: Schwartz, P., Wesselschmidt, R. (eds) Human Pluripotent Stem Cells. Methods in Molecular Biology, vol 767. Humana Press. https://doi.org/10.1007/978-1-61779-201-4_15
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DOI: https://doi.org/10.1007/978-1-61779-201-4_15
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