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The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing

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Surface Plasmon Resonance

Part of the book series: Methods in Molecular Biology ((MIMB,volume 627))

Abstract

This chapter presents an introduction to the kinetic analysis of SPR biosensor data for the determination of affinity and kinetic rate constants of biomolecular interactions between an immobilized and a soluble binding partner. The need to be aware of and critically test the assumptions underlying the analysis models is emphasized and the consequences for the experimental design are discussed. The two most common sources of deviation in SPR surface binding kinetics from the ideal pseudo-first-order binding kinetics of bimolecular reactions are mass transport limitations and the heterogeneity of the surface sites. These problems are intrinsic to the use of a biosensor surface for characterizing interactions. The effect of these factors on the observed binding kinetics, and strategies to account for them are reviewed, both in the context of mathematical data analysis, as well as the design of the experiments and controls.

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Acknowledgment

This research was supported by the Intramural Research Program of the National Institute of Bioengineering and Physical Science, National Institutes of Health.

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Authors and Affiliations

  1. Dynamics of Macromolecular Assembly, Laboratory of Bioengineering and Physical Science, National Institute of Biomedical Imaging and Bioengineering (NIBIB), National Institutes of Health, Bethesda, MD, USA

    Peter Schuck & Huaying Zhao

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  1. Peter Schuck
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  2. Huaying Zhao
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  1. Dept. Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, Utrecht, 3585 CA, Netherlands

    Nico J. Mol

  2. Dept. Pharmaceutical Sciences, Utrecht University, Sorbonnelaan 16, Utrecht, 3585 CA, Netherlands

    Marcel J. E. Fischer

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Schuck, P., Zhao, H. (2010). The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing. In: Mol, N., Fischer, M. (eds) Surface Plasmon Resonance. Methods in Molecular Biology, vol 627. Humana Press. https://doi.org/10.1007/978-1-60761-670-2_2

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  • DOI: https://doi.org/10.1007/978-1-60761-670-2_2

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