Abstract
A protocol for production of bovine embryos from oocytes collected from ovaries obtained from an abattoir is described. The protocol includes methods for in vitro maturation of oocytes, capacitation of sperm, fertilization, and development of the resultant embryos to the blastocyst stage. The protocol can be easily modified to use oocytes collected by ultrasound-guided follicular aspiration.
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Tríbulo, P., Rivera, R.M., Ortega Obando, M.S., Jannaman, E.A., Hansen, P.J. (2019). Production and Culture of the Bovine Embryo. In: Herrick, J. (eds) Comparative Embryo Culture. Methods in Molecular Biology, vol 2006. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9566-0_8
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DOI: https://doi.org/10.1007/978-1-4939-9566-0_8
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-9565-3
Online ISBN: 978-1-4939-9566-0
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