Abstract
The wheat genome encodes some 100,000 genes. To understand how the expression of these genes is regulated it will be necessary to carry out many genetic transformation experiments. Robust protocols that allow scientists to transform a wide range of wheat genotypes are therefore required. In this chapter, we describe a protocol for biolistic transformation of wheat that uses immature embryos and small quantities of DNA cassettes. An original method for DNA cassette purification is also described. This protocol can be used to transform a wide range of wheat genotypes and other related species.
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Acknowledgments
The authors thank the glasshouse team for optimal plant breeding. This work was supported by INRA BAP (Biology and Plant Breeding) department grants and by the program Investments for the Future (grant ANR-11-BTBR-0006-GENIUS) managed by the French National Research Agency.
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Tassy, C., Barret, P. (2017). Biolistic Transformation of Wheat. In: Bhalla, P., Singh, M. (eds) Wheat Biotechnology. Methods in Molecular Biology, vol 1679. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7337-8_9
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DOI: https://doi.org/10.1007/978-1-4939-7337-8_9
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