Abstract
Our understanding of colitis-associated carcinoma (CAC) has benefited substantially from mouse models that faithfully recapitulate human CAC. Chemical models, in particular, have enabled fast and efficient analysis of genetic and environmental modulators of CAC without the added requirement of time-intensive genetic crossings. Here we describe the Azoxymethane (AOM)/Dextran Sodium Sulfate (DSS) mouse model of inflammatory colorectal cancer.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Siegel R, Ma J, Jemal A (2014) Cancer statistics, 2014. CA Cancer J Clin 64:9–29
Danese S, Malesci A, Vetrano S (2011) Colitis-associated cancer: the dark side of inflammatory bowel disease. Gut 60:1609–1610
Danese S, Mantovani A (2010) Inflammatory bowel disease and intestinal cancer : a paradigm of the Yin – Yang interplay between inflammation and cancer. Oncogene 29:3313–3323
Terzić J, Grivennikov S, Karin E, Karin M (2010) Inflammation and colon cancer. Gastroenterology 138:2101–2114
Eaden JA, Abrams KR, Mayberry JF (2001) The risk of colorectal cancer in ulcerative colitis : a meta analysis. Gut 48:526–535
Ekbom A, Helmick C, Zack M, Adami H (1990) Ulcerative colitis and colorectal cancer. N Engl J Med 323:1228–1233
Hovde O, Kempski-Monstad I, Småstuen MC, Solberg IC, Henriksen M, Jahnsen J et al (2013) Mortality and causes of death in Crohn’s disease: results from 20 years of follow-up in the IBSEN study. Gut 63:771–775
Beaugerie L, Svrcek M, Seksik P, Bouvier AM, Simon T, Allez M et al (2013) Risk of colorectal high-grade dysplasia and cancer in a prospective observational cohort of patients with inflammatory bowel disease. Gastroenterology 145:166–175
Gyde SN, Prior P, Allan RN, Stevens A, Jewell DP, Truelove SC et al (1988) Colorectal cancer in ulcerative colitis: a cohort study of primary referrals from three centres. Gut 29:206–217
Lakatos PL, Lakatos L (2008) Risk for colorectal cancer in ulcerative colitis: changes, causes and management strategies. World J Gastroenterol 14:3937–3947
Jess T, Rungoe C, Peyrin-Biroulet L (2012) Risk of colorectal cancer in patients with ulcerative colitis: a meta-analysis of population-based cohort studies. Clin Gastroenterol Hepatol 10:639–645
Tanaka T, Kohno H, Suzuki R, Yamada Y, Sugie S, Mori H (2003) A novel inflammation-related mouse colon carcinogenesis model induced by azoxymethane and dextran sodium sulfate. Cancer Sci 94:965–973
De Robertis M, Massi E, Poeta ML, Carotti S, Morini S, Cecchetelli L et al (2011) The AOM/DSS murine model for the study of colon carcinogenesis: from pathways to diagnosis and therapy studies. J Carcinog 10:9
Chen J, Huang XF (2009) The signal pathways in azoxymethane-induced colon cancer and preventive implications. Cancer Biol Ther 14:1313–1317
Suzuki R, Kohno H, Sugie S, Nakagama H, Tanaka T (2006) Strain differences in the susceptibility to azoxymethane and dextran sodium sulfate-induced colon carcinogenesis in mice. Carcinogenesis 1:162–169
Sohn OS, Fiala ES, Requeijo SP (2001) Differential effects of CYP2E1 status on the metabolic activation of the colon carcinogens azoxymethane. Cancer Res 61:8435–8440
Okayasu I, Hatakeyama S, Yamada M, Ohkusa T, Inagaki Y, Nakaya R (1999) A novel method in the induction of reliable experimental acute and chronic colitis in mice. Gastroenterology 98:694–702
Fukata M, Chen A, Vamadevan AS, Cohen J, Breglio K, Krishnareddy S et al (2007) Toll-like receptor-4 promotes the development of colitis-associated colorectal tumors. Gastroenterology 133:1869–1881
Greten FR, Eckmann L, Greten TF, Park JM, Li ZW, Egan LJ et al (2004) IKKβ links inflammation and tumorigenesis in a mouse model of colitis-associated cancer. Cell 118:285–296
Grivennikov S, Karin E, Terzic J, Mucida D, Yu GY, Vallabhapurapu S et al (2009) IL-6 and Stat3 are required for survival of intestinal epithelial cells and development of colitis-associated cancer. Cancer Cell 15:103–113
Barrett CW, Ning W, Chen X, Smith JJ, Washington MK, Hill KE et al (2012) Tumor suppressor function of the plasma glutathione peroxidase Gpx3 in colitis-associated carcinoma. Cancer Res 73:1245–1255
Uronis JM, Mühlbauer M, Herfarth HH, Rubinas TC, Jones GS, Jobin C (2009) Modulation of the intestinal microbiota alters colitis-associated colorectal cancer susceptibility. PLoS One 4, e6026
Barrett CW, Fingleton B, Williams A, Ning W, Fischer M, Washington MK et al (2011) MTGR1 is required for tumorigenesis in the murine AOM/DSS colitis-associated carcinoma model. Cancer Res 71:1302–1312
Whitten C, Williams A, Williams CS (2010) Murine colitis modeling using dextran sulfate sodium. J Vis Exp 35:5–8
Acknowledgments
This work was supported by the National Institutes of Health grants DK080221 (C.S.W.), 1F30DK096718-01A1 (B.P.), T32 GM07347 (NIH/NIGMS) (B.P.), Merit Review Grants from the Office of Medical Research, Department of Veterans Affairs 1I01BX001426 (C.S.W.), and ACS-RSG 116552 (C.S.W.).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2016 Springer Science+Business Media New York
About this protocol
Cite this protocol
Parang, B., Barrett, C.W., Williams, C.S. (2016). AOM/DSS Model of Colitis-Associated Cancer. In: Ivanov, A. (eds) Gastrointestinal Physiology and Diseases. Methods in Molecular Biology, vol 1422. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3603-8_26
Download citation
DOI: https://doi.org/10.1007/978-1-4939-3603-8_26
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3601-4
Online ISBN: 978-1-4939-3603-8
eBook Packages: Springer Protocols