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Fusion-Permissive Protoplasts

A Plant System for Studying Cell Fusion

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Cell Fusion

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Abstract

While artificial systems are useful for delineating the fusogenic properties of specific lipids or proteins, they cannot encompass the complexity of A. living cell membrane. To address basic questions such as what renders the plasma membrane fusion permissive and what regulates the fusion permissive state in vivo, A. highly fusion permissive system was needed. Since under nonpathological conditions there are only A. few times in the life of an organism in which cells are capable of undergoing fusion (e.g., fertilization and muscle maturation), few model systems are available. A. new method has been developed for culturing cells of wild carrot (Daucus carotaL.), so that they yield fusogenic or fusion permissive protoplasts (Boss et al, 1984a). The fusion yield (fused protoplasts/fused + nonfused protoplasts) is greater than 50%. Fusion is calcium stimulated, enhanced by the calcium ionophore, A23187, and inhibited by EGTA and calmodulin antagonists (Boss and Grimes, 1985; Grimes and Boss, 1985).

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Authors and Affiliations

  1. Botany Department, North Carolina State University, Raleigh, North Carolina, 27695, USA

    Wendy F. Boss

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  1. Wendy F. Boss
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  1. American National Red Cross, Rockville, Maryland, USA

    Arthur E. Sowers

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Boss, W.F. (1987). Fusion-Permissive Protoplasts. In: Sowers, A.E. (eds) Cell Fusion. Springer, Boston, MA. https://doi.org/10.1007/978-1-4757-9598-1_7

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  • DOI: https://doi.org/10.1007/978-1-4757-9598-1_7

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