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Methods for the Determination of Ethanol and Acetaldehyde

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The Biology of Alcoholism

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Abstract

New methods for the determination of the ethanol concentration of biological specimens or adaptations of older procedures are published frequently. As technology has advanced, analysts have applied the newly developed concepts to the analysis of ethanol. Illustrative and typical methods will be detailed subsequently and their relative attributes and limitations will be discussed. Despite all efforts to be inclusive, the probability is high that a significant development will occur between the writing and publication of this review.

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References

  • Anders, M. W., and Mannering, G. W., 1967. In Progress in Chemical Toxicology (A. Stolman, ed.), Vol. 3, pp. 121–218, Academic Press, New York.

    Google Scholar 

  • Borkenstein, R. F., 1963. Breath Tests to Determine Alcoholic Influence, Stephenson Corp., Red Bank, pp. 33–34.

    Google Scholar 

  • Borkenstein, R. F., and Smith, H. W., 1961. The breathalyzer and its application, Medicine, Science and the Law 1: 13.

    Google Scholar 

  • Bradford, L. W., 1966. Preservation of blood samples containing alcohol, J. Forensic Sci. 11: 214–216.

    Google Scholar 

  • Brink, N. G., Bonnichsen, R., and Theorell, H., 1954. A modified method for the enzymatic microdetermination of ethanol, Acta Pharmacol. Toxicol. 10: 223.

    Article  Google Scholar 

  • Burbridge, T. N., Hine, C. N., and Schick, A. F., 1950. A simple spectrophotometric method for the determination of acetaldehyde in blood, J. Lab. Clin. Med. 35: 985–987.

    Google Scholar 

  • Burger, J. D., 1968. Gas chromatographic behavior of various types of organic compounds on Porapak Q, J. Gas Chromatogr. 6: 177–178.

    Google Scholar 

  • Casier, H., and Delaunois, A. L., 1947. L’Intoxication par l’Alcohol Ethylique, Paris,Masson and Cie.

    Google Scholar 

  • Conway, E. J., 1963. Microdiffusion Analysis and Volumetric Error, Chemical Publishing Co., Inc., New York.

    Google Scholar 

  • Curry, A., Walker, G. W., and Simpson, G., 1966. Determination of ethanol in blood by gas chromatography, Analyst 9: 742–743.

    Article  Google Scholar 

  • Dubowski, K. M., 1959. A.M.A. Committee on Medicolegal Problems: Chemical Tests for Intoxication Manual, American Medical Association, Chicago.

    Google Scholar 

  • Dubowski, K. M., 1964. Specificity of breath alcohol analyses, J. Am. Med. Assoc. 189: 1039.

    Google Scholar 

  • Dubowski, K. M., 1968. A.M.A. Committee on Medicolegal Problems: Chemical Tests for Intoxication Manual, American Medical Association, Chicago.

    Google Scholar 

  • Dubowski, K. M., and Shupe, L. M., 1952. Improved semimicro distillation apparatus, Am. J. Clin. Pathol. 22: 709.

    Google Scholar 

  • Dubowski, K. M., and Withrow, J. R., 1952. A photometric microdetermination method for ethyl alcohol in biological materials, Proc. Am. Acad. Forensic Sci. 2: 323.

    Google Scholar 

  • Duritz, G., and Truitt, E. B., 1964. A rapid method for the simultaneous determination of acetaldehyde and ethanol in blood using gas chromatography, Quart. J. Studies Alc. 25: 498.

    Google Scholar 

  • Ebel, H. and Schleyer, F., 1956. Blutalkohol,2nd ed., p. 40, Stuttgart, Georg Thieme Verlag.

    Google Scholar 

  • Ellis, F. W., and Hill, J. B., 1969. An automated fluorometric procedure for the enzymatic determination of ethanol in fingertip blood, Clin. Chem. 15: 91–101.

    Google Scholar 

  • Freimuth, H. C., Watts, S. R., and Fisher, R. S., 1958. Alcohol and highway fatalities, J. Forensic Sci. 3: 65.

    Google Scholar 

  • Freund, G., and O’Halloren, P., 1965. Acetaldehyde concentrations in alveolar air following a standard dose of ethanol in man, J. Lipid Res. 6: 471–477.

    Google Scholar 

  • Friedemann, T. E., and Dubowski, K. M., 1959. Chemical testing procedures for the determination of ethyl alcohol, J. Am. Med. Assoc. 170: 47.

    Article  Google Scholar 

  • Glendening, B. L., and Waugh, T. C., 1965. The stability of ordinary blood alcohol samples held various periods of time under different conditions, J. Forensic Sci. 10: 192.

    Google Scholar 

  • Goldberg, L., and Rydberg, U., 1966. In Automation in Analytical Chemistry (Technicon Symposia, 1965) (L. T. Sheggs, ed.), pp. 595–600, Mediad, New York.

    Google Scholar 

  • Harger, R. N., 1961. In Toxicology-Mechanisms and Analytical Methods (C. P. Stewart and A. Stolman, eds.), Vol. 2, pp. 85–151, Academic Press, New York.

    Google Scholar 

  • Harger, R. N., and Forney, R. B., 1967. In Progress in Chemical Toxicology (A. Stolman, ed.), Vol. 3, pp. 1–61, Academic Press, New York.

    Google Scholar 

  • Harger, R. N., and Hulpieu, H. R., 1956. In Alcoholism (G. N. Thompson, ed.), pp. 103–232, Charles C Thomas, Springfield, Ill.

    Google Scholar 

  • Hodnett, N., and Sunshine, I., 1970. In Biochemical and Clinical Aspects of Alcohol Metabolism ( V. M. Sardesal, ed.), Charles C Thomas, Springfield, Ill.

    Google Scholar 

  • Howes, J. R., Hallett, R. A., and Lucas, D. M., 1967. A study of the accuracy of the Breathalyzer as operated by police personnel, J. Forensic Sci. 12: 444–453.

    Google Scholar 

  • Huet, M., 1967. In Automation in Analytical Chemistry (Technicon Symposia 1966) (E. Kawerau, ed.), Vol. 2, pp. 155–158, Mediad, New York.

    Google Scholar 

  • Kaye, S., 1954. In Legal Medicine (R. B. H. Gradwohl, ed.), Vol. 1, pp. 772–774, C. V. Mosby Co., St. Louis.

    Google Scholar 

  • Kozelka, F. L., and Hine, C. H., 1941. Method for Determination of Ethyl Alcohol for Medicolegal Purposes, Ind. Eng. Chem. Anal. Ed. 13: 905.

    Article  Google Scholar 

  • Leithoff, H., 1963. Eine Automatische Methode der Blut-alkoholbestimmung, Med. Welt. 17: 959.

    Google Scholar 

  • Lundquist, F., 1958. Enzymatic determination of acetaldehyde in blood, Biochem. J. 68: 172–177.

    Google Scholar 

  • Malmstadt, H. V., and Hadjiioannou, T. P., 1962. Specific enzymatic determination of alcohol in blood by an automatic spectrophotometric reaction rate method, Anal. Chem. 34: 455–458.

    Article  Google Scholar 

  • Machata, G., 1964. Uber die Gaschromatographische Blutalkoholbestimmung, Blutalkohol 4: 252–260.

    Google Scholar 

  • Mather, A., and Assimos, A., 1965. Evaluation of gas-liquid chromatography in assays for blood volatiles, Clin. Chem. 11: 1023–1035.

    Google Scholar 

  • Nadeau, G., Fortin, B., and Dugal, P., 1962. Continuous in vivo estimation of blood alcohol, Clin. Chem. 8: 72.

    Google Scholar 

  • Parker, K. D., Fontan, C. R., Yee, J. L., and Kirk, P. L., 1962. Gas chromatographic determination of ethl alcohol in blood for medicolegal purposes, Anal. Chem. 34: 1234.

    Google Scholar 

  • Pleuckhahn, V. D., 1968. The evaluation of autopsy blood alcohol levels, Medicine, Science and the Law 8: 168–176.

    Google Scholar 

  • Roach, M. K., and Creaven, P. J., 1968. A micro-method for the determination of acetaldehyde and ethanol in blood, Clin. Chim. Acta 21: 275–278.

    Google Scholar 

  • Skeggs, L. T., 1957. An automatic method for colorimetric analysis, Am. J. Clin. Pathol. 28: 311–322.

    Google Scholar 

  • Smith, H. W., 1965. In Methods of Forensic Science (A. S. Curry, ed.), Vol. 4, pp. 1–97, Interscience Publishers, London.

    Google Scholar 

  • Stotz, E., 1943. A colorimetric determination of acetaldehyde in blood, J. Biol. Chem. 148: 585–591.

    Google Scholar 

  • Stewart, R. D., and Boettner, E. A., 1964. Expired-air acetone in diabetes mellitus, N. Eng. J. Med. 270: 1035–1038.

    Article  Google Scholar 

  • Stunner, W. Q., and Coumbis, R. J., 1966. The quantitation of ethyl alcohol in vitreous humor and blood by gas chromatography, Am. J. Clin. Pathol. 46: 349–351.

    Google Scholar 

  • Sunshine, I., and Nenad, R., 1953. A modification of Winneck’s method for the rapid

    Google Scholar 

  • determination of ethyl alcohol in biological fluids, Anal. Chem. 25: 653.

    Google Scholar 

  • Syed, R. A., 1968. Enzymatic determination of ethyl acohol by an automated fluorometric technique. In Automation in Analytical Chemistry, Vol. 1, pp. 571–577, Mediad, New York.

    Google Scholar 

  • Turkel, H. W, and Gifford, H., 1957. Erroneous blood findings at autopsy, J. Am. Med. Assoc. 164: 1077.

    Article  Google Scholar 

  • Wallace, J. E., and Dahl, E. V., 1966. Rapid vapor phase method for determining ethanol in blood and urine using gas chromatography, Am. J. Clin. Pathol. 46: 152–154.

    Google Scholar 

  • Widmark, E. M. P., 1922. Eine Mikromethode zur Bestimmung von Äthylalkohol im Blut, Biochem. Z. 131: 473.

    Google Scholar 

  • Williams, L. A., Linn, R. A., and Zak, B., 1958. Determination of ethanol in fingertip quantities of blood, Clin. Chim. Acta 3: 169–172.

    Article  Google Scholar 

  • Worthington, 1967. In Glucostat Determinations, pp. 13–14, Worthington Biochemical Corp., Freehold, N.J.

    Google Scholar 

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Author information

Authors and Affiliations

  1. Cuyahoga County Coroner’s Laboratory School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA

    Irving Sunshine

  2. Cuyahoga County Coroner’s Laboratory, Cleveland, Ohio, USA

    Nicholas Hodnett

Authors
  1. Irving Sunshine
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  2. Nicholas Hodnett
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Editor information

Editors and Affiliations

  1. Division of Alcoholism and Drug Dependence Department of Psychiatry, State University of New York Downstate Medical Center, Brooklyn, New York, USA

    Benjamin Kissin  & Henri Begleiter  & 

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© 1971 Springer Science+Business Media New York

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Sunshine, I., Hodnett, N. (1971). Methods for the Determination of Ethanol and Acetaldehyde. In: Kissin, B., Begleiter, H. (eds) The Biology of Alcoholism. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6525-3_16

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  • DOI: https://doi.org/10.1007/978-1-4615-6525-3_16

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4615-6527-7

  • Online ISBN: 978-1-4615-6525-3

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