Abstract
Salt-loaded cells of the cyanobacterium Synechocystis sp. PCC 6803 accumulate mainly the osmoprotective compound glucosylglycerol (GG). The enzymes involved in GG biosynthesis were found to be strictly dependent on enhanced salt concentrations. By modulation of the NaCl concentration in vitro,it was possible to activate and to inactivate the enzymes. Using salt-sensitive mutants generated by random cartridge mutagenesis, the genes encoding for the GG synthesizing enzymes were cloned. Their expression was about threefold enhanced in salt stressed cells. A model is presented showing the dual role of NaCl as a stressor and a regulator in salt-stressed cells.
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Hagemann, M., Schoor, A., Effmert, U., Zuther, E., Marin, K., Erdmann, N. (1999). The Biochemistry and Genetics of the Glucosylglycerol Synthesis in Synechocystis sp. PCC 6803. In: Peschek, G.A., Löffelhardt, W., Schmetterer, G. (eds) The Phototrophic Prokaryotes. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-4827-0_67
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DOI: https://doi.org/10.1007/978-1-4615-4827-0_67
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