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Preparation and Detection of Glycan-Binding Activity of Influenza Virus

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Lectin Purification and Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2132))

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Abstract

We describe a method to detect influenza virus using an evanescent-field-activated fluorescence scanner type glycan array and ELISA system. Neoglycoprotein was prepared by combination of organic chemistry and biomaterial preparation. These ligands were spotted on a glass plate or plastic well to make a glycan array and ELISA plate. We detected cultured influenza virus using glycan array and ELISA. Then, we showed that the neoglycoprotein binds to Cy3-labeled hemagglutinins (H1 and H5), a NeuAcα2,6LacNAc or NeuAcα2,3LacNAc recognized protein, as detected.

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Authors and Affiliations

  1. Biotechnology Research Institute for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

    Shin-ichi Nakakita

  2. Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Pathumthani, Thailand

    Nongluk Sriwilaijaroen

  3. College of Life and Health Sciences, Chubu University, Kasugai, Aichi, Japan

    Yasuo Suzuki

  4. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

    Jun Hirabayashi

Authors
  1. Shin-ichi Nakakita
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  2. Nongluk Sriwilaijaroen
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  3. Yasuo Suzuki
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  4. Jun Hirabayashi
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Corresponding author

Correspondence to Shin-ichi Nakakita .

Editor information

Editors and Affiliations

  1. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

    Jun Hirabayashi

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Nakakita, Si., Sriwilaijaroen, N., Suzuki, Y., Hirabayashi, J. (2020). Preparation and Detection of Glycan-Binding Activity of Influenza Virus. In: Hirabayashi, J. (eds) Lectin Purification and Analysis. Methods in Molecular Biology, vol 2132. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0430-4_49

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  • DOI: https://doi.org/10.1007/978-1-0716-0430-4_49

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0429-8

  • Online ISBN: 978-1-0716-0430-4

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