Abstract
Basidiomycetes of various species and their wide range of pharmaceutically interesting products in the last decades represent one of the most attractive groups of natural products in Asia and North America. Production of fungal fruit bodies using farming technology is hardly covering the market. Comprehensive solid-state technologies and bioreactors are the most promising part for fast and large amount of cultivation of medicinal fungi biomass and its pharmaceutically active products. Wood, agriculture, and food industry wastes represent the main substrates that are in this process delignified and enriched in proteins and highly valuable pharmaceutically active compounds. Research in physiology, basic and applied studies in fungal metabolism, process engineering aspects, and clinical studies in the last two decades represent large contribution to the development of these potentials that initiate the development of new drugs and some of the most attractive over-the-counter human and veterinary remedies. Present article is an overview of the achievements in solid-state technology of the most relevant medicinal mushroom species production in bioreactors.
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Abbreviations
- ψ :
-
Water potential
- a w :
-
Water activity
- EPS:
-
Exopolysaccharide
- M :
-
Water molecular mass
- NGF:
-
Nerve growth factor
- P :
-
Water vapor pressure
- P 0 :
-
Water vapor pressure of pure water under the same condition
- PSK:
-
Polysaccharide krestin from Trametes versicolor
- PSP:
-
Polysaccharopeptide from Trametes versicolor
- R :
-
Gas constant (8.31 J/mol K)
- SmB:
-
Submerged bioprocessing
- SSC:
-
Solid-state cultivation
- T :
-
Absolute temperature
References
Wasser SP (2002) Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides. Appl Microbiol Biotechnol 60:258–274
Bensky D, Gamble A (1993) In: Chinese materia medica. 2nd edn. Eastland Press, Seattle, pp 24–34
Wasser SP, Weiss AL (1997) Medicinal mushrooms – Ganoderma lucidum, Reishi mushroom. Pedeifus Publishing House, Haifa
Ooi VEC, Fang Liu F (2000) Immunomodulation and anti-cancer activity of polysaccharide-protein complexes. Curr Med Chem 7:715–729
Hobbs C (1995) Medicinal mushrooms: an exploration of tradition, healing and culture. Botanica Press, Santa Cruz
Mizuno T (1999) The extraction and development of antitumor-active polysaccharides from medicinal mushrooms in Japan. Int J Med Mush 1:9–29
Bohn JA, BeMiller JN (1995) (1-3)-β-D-Glucans as biological response modifiers: a review of structure-functional activity relationships. Carbohydr Polym 28:3–14
Li M, Lei L, Liang D (1999) Effect of Ganoderma polysaccharide on intracellular free calcium in murine peritoneal macrophages. Chin Pharm J 34:805–807
Maeda YY, Takahama S, Kohara Y, Yonekawa H (1996) Two genes controlling acute phase responses by the antitumour polysaccharide lentinan. Immunogenetics 43:215–219
Maeda YY, Yonekawa H, Chihara G (1994) Application of lentinan as cytokine inducer and host defense potentiator in immunotherapy of infectious diseases. In: Masishi KW (ed) Immunotherapy of infections. Marcel Dekker Press, New York, pp 261–279
Chen JZ, Seviour R (2007) Medicinal importance of fungal ß-(1-3), (1-6)-glucans. Mycol Res 111:635–652
Moradali MF, Mostafavi H, Ghods S, Hedjaroude GA (2007) Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi). Int Immunopharmacol 7:701–724
Kidd PH (2000) The use of mushroom glucans and proteoglycans in cancer treatment. Altern Med Rev 5:4–27
Mitchell DA, Berovic M (2018) Solid state cultivation. In: Berovic M, Hewitt CJ (eds) Principles of comprehensive biochemical engineering. University of Ljubljana Press, Ljubljana, pp 225–262
Thomas L, Larroche C, Pandey A (2013) Current developments in solid state bioprocessing. Biochem Eng J 81:146–161
Soccol R, Scopel Ferreira da Costa E, Junior Letti LA, Grace Karp S, Lorenci Woiciechowski A, Vandenberghe Porto de Souza L (2017) Recent developments and innovations in solid state cultivation. Biotechnol Res Inn 1:52–71
Arora S, Rani R, Ghosh S (2018) Bioreactors in solid state cultivation technology: design, applications and engineering aspects. J Biotechnol 26:916–934
Hu C, Meguro S, Kawachi S (2004) Effects of physical properties of wood on the water activity of wood meal media for the cultivation of edible mushrooms. J Wood Sci 50:365–370
Battaglino RA, Huergo M, Pilosof AMR, Bartholomai GB (1991) Culture requirements for the production of protease by Aspergillus oryzae in solid state cultivation. Appl Microbiol Biotechnol 35(3):292–296
Durand A (2003) Bioreactor designs for solid state fermentation. Biochem Eng J 13:113–125
Velioglu Z, Urek RO (2015) Optimization of cultural conditions for biosurfactant production by Pleurotus djamor in solid state cultivation. J Biosci Bioeng 120:526–531
Abdullah AL, Tengerdy RP, Murphy VG (1984) Optimization of solid substrate bioprocessing of wheat straw. Biotecnol Bioeng 27:20–27
Ashok A, Doriya K, Ram Mohan Rao D, Kumar DS (2017) Design of solid state bioreactor for industrial applications: an overview to conventional bioreactors. Biocatal Agric Biotechnol 9:11–18
Mitchell DA, Berovic M, Krieger N (2006) Introduction to solid state bioprocessing bioreactors. In: Mitchell DA, Krieger N, Berovic M (eds) Solid state bioprocessing bioreactors: fundamentals of design and operation. Springer, Berlin, pp 125–132
Lonsane BK, Saucedo-Castaneda G, Raimbault M, Roussos S, Viniegra-Gonzalez G, Ghildyal NP, Ramakrishna M, Krishnaiah MM (1992) Scale-up strategies for solid state cultivation systems. Process Biochem 27:259–273
Fung CJ, Mitchell DA (1995) Baffles increase performance of solid state cultivation in rotating drums. Biotech Tech 9:295–298
Berovic M, Ostroversnik H (1997) Production of Aspergillus niger pectolytic enzymes by solid state cultivation of apple pomace. J Biotechnol 53:47–53
Habijanic J, Berovic M (2000) The relevance of solid state substrate moisturing on Ganoderma lucidum biomass cultivation. Food Technol Biotechnol 38:225–228
Švagelj M, Berovic M, Boh B, Menard A, Simcic S, Wraber B (2008) Solid state cultivation of Grifola frondosa (Dicks: Fr) S.F. Gray biomass and immunostimulatory effects of fungal intra-and extracellular ß-polysaccharides. N Biotechnol 25:150–155
Boh B (2006) Use of waste materials from agriculture for cultivation of edible and medicinal mushrooms. In: Information study and reference database. Bistra Press, Ptuj, pp 180–186
Mizuno T, Wang G, Zhang J, Kawagishi H, Nishitoba T, Li J (1995) Reishi, Ganoderma lucidum and Ganoderma tsugae: bioactive substance and medicinal effects. Food Rev Int 11:151–166
Lee KM, Lee SY, Lee HY (1999) Bistage control of pH for improving exopolysaccharide production from mycelia of Ganoderma lucidum in an air-lift fermentor. J Biosci Bioeng 88:646–650
Wagner R, Mitchell DA, Lanzi Sassaki G, Lopes de Almeida Amazonas MA, Berovic M (2003) Submerged cultivation of Ganoderma lucidum. Food Technol Biotechnol 41:371–382
Hsieh C, Yang FC (2004) Reusing soy residue for the solid state bioprocessing of Ganoderma lucidum. Bioresour Technol 91:105–109
Song M, Kim N, Lee S, Hwang S (2007) Use of whey permeate for cultivating Ganoderma lucidum mycelia. J Dairy Sci 90:2141–2146
Montoya S, Sanchez OJ, Levin L (2013) Polysaccharide production by submerged and solid state cultures from several medicinal higher basidiomycetes. Int J Med Mushrooms 15:71–79
Montoya-Barreto S, Varon Lopez M, Levin L (2008) Effect of culture parameters on the production of the edible mushroom Grifola frondosa (maitake) in tropical weathers. World J Microbiol Biotechnol 24:1361–1366
Postemsky PD, Bidegain MA, González-Matute R, Figlas ND, Cubitto MA (2017) Pilot-scale bioconversion of rice and sunflower agro-residues into medicinal mushrooms and laccase enzymes through solid state bioprocessing with Ganoderma lucidum. Bioresour Technol 231:85–93
Habjanic J, Berovic M (2002) Process of cultivation of fungus Ganoderma lucidum on a solid cultivation substrate. SI Patent 20923, 31 Dec 2002
**ng ZT, Cheng JH, Tan Q, Pan YJ (2006) Effect of nutritional parameters on laccase production by the culinary and medicinal mushroom, Grifola frondosa. World J Microbiol Biotechnol 22:799–806
Habijanic J, Švagelj M, Berovic M, Boh B (2009) Submerged and solid state cultivation of bioactive extra – and intracellular polysaccharides of medicinal mushrooms Ganoderma lucidum (W. Curt.: Fr.) P. Karst. and Grifola frondosa (Dicks.: Fr.) S. F. Gray (Aphyllophoromycetideae). Int J Med Mush 11(4):1–10
Knežević A, Milovanović I, Stajić M, Vukojević J (2013) Trametes suaveolens as ligninolytic enzyme producer. J Nat Sci 124:437–444
Stoilova I, Krastanov A, Stanchev V (2010) Properties of crude laccase from Trametes versicolor producedby solid-substrate cultivation. Adv Biosci Biotechnol 1:208–215
Dinis M, Bezerra RM, Nunes F, Dias AA, Guedes CV, Ferreira LM, Cone JW, Marques GS, Barros AR, Rodrigues MA (2009) Modification of wheat straw lignin by solid state cultivation with white-rot fungi. Bioresour Technol 100:4829–4835
Souza ÉSD, Sampaio IDL, Freire AKDL, Khell B, Silva S, Sobrinho ADS, Lima AM, Souza JVB (2011) Production of Trametes versicolor laccase by solid state cultivation using a fixed-bed bioreactor, Food Agric Environ 9: 55-58.
Rakus J, Berovic M, Golob J (2016) Extraction of fungal polysaccharides from solid state cultivated mycelia Trametes versicolor (Agaricomycetes). Int J Med Mushrooms 18:509–519
Ko HH, Hung CF, Wang JP, Lin CN (2008) Antiinflammatory triterpenoids and steroids from Ganoderma lucidum and G. tsugae. Phytochemistry 69:234–239
Berovic M, Habijanic J, Boh B, Wraber B, Petravic-Tominac V (2012) Production of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (W.Curt. :Fr.) P. Karst. (Higher Basidiomycetes), biomass and polysaccharides by solid state cultivation. Int J Med Mushrooms 14:513–520
Gerbec B, Tavčar E, Gregori A, Kreft S, Berovic M (2015) Solid state cultivation of Hericium erinaceus biomass and erinacine A production. J Bioprocess Biotech 5:1–5
Han J (2003) Solid state cultivation of cornmeal with the basidiomycete Hericium erinaceum for degrading starch and upgrading nutritional value. Int J Food Microbiol 80:61–66
Gregori A (2014) Cordycepin production by Cordyceps militaris cultivation on spent brewery grains. Acta Biol Slovenica 57:45–52
Ardigo W (2016) In: Wasser SP, Volz PA (eds) Healing with medicinal mushrooms. Youcanprint Self-Publishing Press, Roma, pp 313–321
Chu KK, Ho SS, Chow AH (2002) Trametes versicolor: a medicinal mushroom with promising immunotherapeutic values. J Clin Pharmacol 42:976–984
Cheng KF, Leung PC (2008) General review of polysaccharopeptides (PSP) from C. versicolor: pharmacological and clinical studies. Cancer Ther 6:117–130
Ng TB (1998) A review of research on the protein-bound polysaccharide (polysaccharopeptide, PSP) from the mushroom Trametes versicolor (Basidiomycetes: Polyporaceae). Gen Pharmacol 30:1–4
Kidd PM (2005) The use of mushroom glucans and proteoglycans in cancer treatment. Altern Med Rev 1:4–27
Cui J, Chisti Y (2003) Polysaccharopeptides of Trametes versicolor: physiological activity, uses, and production. Biotechnol Adv 21:109–122
Price LA, Wenner CA, Sloper DT (2010) Role for toll-like receptor 4 in TNF-alpha secretion by murine macrophages in response to polysaccharide Krestin, a Trametes versicolor mushroom extract. Fitoterapia 81:914–919
Kawagishi H, Shimada A, Shirai R, Okamoto K, Ojima F, Sakamoto H, Furukawa S (1994) Erinacines A, B and C, strong stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum. Tetrahedron Lett 35(10):1569–1572
Kawagishi H, Shimada A, Shizuki K, Mori H (1996) Erinacine D, a stimulator of NGF-synthesis, from the mycelia of Hericium erinaceum. Heterocycl Commun 2:51–54
Kawagishi H, Shimada A, Hosokawa S, Mori H (1996) Erinacines E, F and G, stimulators of nerve growth factor (NGF)-synthesis, from the mycelia of Hericium erinaceum. Tetrahedron Lett 37:7399–7402
Kawagishi H, Masui A, Tukuyama S, Nakamura T (2006) Erinacines J and K from the mycelia of Hericium erinaceum. Tetrahedron Lett 62:8463–8466
Kenmoku H, Sassa T, Kato N (2000) Isolation of erinacine P, a new parental metabolite of cyathane-xylosides, from Hericium erinaceum and its biomimetic conversion into erinacines A and B. Tetrahedron Lett 41:4389–4393
Kenmoku H, Shimai T, Toyomasu T, Kato N, Sassa T (2002) Erinacine Q, a new erinacine from Hericium erinaceum and its biosynthetic route to erinacine C in the basidiomycete. Biosci Biotechnol Biochem 66:571–575
Lee EW, Shizuki K, Hosokawa S, Suzuki M (2000) Two novel diterpenoids, erinacines H and I from the mycelia of Hericium erinaceum. Biosci Biotechnol Biochem 64:2402–2405
Chen X, Wu G, Huang Z (2013) Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris. Int J Biol Macromol 58:18–22
Guan J, Zhao J, Feng K, Hu DJ, Li SP (2011) Comparison and characterization of polysaccharides from natural and cultured Cordyceps using saccharide map**. Anal Bioanal Chem 399:3465–3474
Russell R, Paterson M (2008) Cordyceps – a traditional Chinese medicine and another fungal therapeutic biofactory? Phytochemistry 69:1469–1495
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Berovic, M. (2019). Cultivation of Medicinal Mushroom Biomass by Solid-State Bioprocessing in Bioreactors. In: Steudler, S., Werner, A., Cheng, J. (eds) Solid State Fermentation. Advances in Biochemical Engineering/Biotechnology, vol 169. Springer, Cham. https://doi.org/10.1007/10_2019_89
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