Abstract
Cyanobacteria are phytoplankton prevalent in freshwater surface waters that can aggregate on the surface as blue-green “scums” or concentrate in surface water supplies as blooms. These secondary metabolites produce various harmful toxins known as cyanotoxins that impair treated water sources and pose a risk to human health and aquatic life by exploiting the food chain. They are of concern because they can produce a variety of toxins that are harmful to both human and animal health and exhibit hepatotoxic, neurotoxic, cytotoxic, and dermatotoxic behavior patterns. With regard to neurotoxicity, the brain can be a crucial target for various cyanotoxins escalating several neurodegenerative diseases. These toxins can block the voltage-gated sodium channels hindering to perform basic neurological functions. Also, the precise target/interacting proteins, the signaling cascades initiating the cell responses, and the downstream pathways of toxicity and cell injury still need to be discovered. Therefore, this chapter summarizes the detection of neurotoxicity using in vitro conditions.
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Acknowledgments
The authors are thankful to Vellore Institute of Technology, Vellore, for providing the necessary facilities to carry out this research.
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Haripriyaa, M., Suthindhiran, K. (2023). In Vitro Assay for Determining Cyanotoxin Using Cell Line Method: Neurotoxicity (Neuro-2a Neuroblastoma Cell). In: Thajuddin, N., Sankara narayanan, A., Dhanasekaran, D. (eds) Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin . Springer, Singapore. https://doi.org/10.1007/978-981-99-4514-6_36
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DOI: https://doi.org/10.1007/978-981-99-4514-6_36
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