Abstract
Cyanobacteria are free living organisms and the probability of finding them in water body is very high. These are proven to be harmful for humans in the form of hepatotoxins, neurotoxins, or alkaloids (forms of cyanotoxins). Thus, detection of these harmful agents becomes a necessity for a healthy environment at all forms of life. This chapter mainly focuses on the serologic methods of detecting cyanotoxins in blood sera using enzyme-linked immunosorbent assay. Although several other methods under analytical tests like chromatographic techniques can be employed to know the accurate concentration, ELISA on the other hand also furnishes good quality results. The following content will describe the protocol of performing experiments to detect cyanotoxins in their classified form.
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References
Chu FS, Fan TS, Zhang GS, Xu YC, Faust S, Mcmahon PL (1987) Improved enzyme-linked immunosorbent assay for aflatoxin Bj in agricultural commodities. J Assoc Off Anal Chem 70(5):854–857
Chu FS, Huang XUAN, Wei RD, Carmichael WW (1989) Production and characterization of antibodies against microcystins. Appl Environ Microbiol 55(8):1928–1933
Fan TS, Zhang GS, Chu FS (1984) An indirect enzyme-linked immunosorbent assay for T-2 toxin in biological fluids. J Food Prot 47(12):964–967
Hilborn ED, Carmichael WW, Yuan M, Azevedo SM (2005) A simple colorimetric method to detect biological evidence of human exposure to microcystins. Toxicon 46(2):218–221
Hilborn ED, Carmichael WW, Soares RM, Yuan M, Servaites JC, Barton HA, Azevedo SMFO (2007) Serologic evaluation of human microcystin exposure. Environ Toxicol 22(5):459–463
Koreivienė J, Belous O (2012) Methods for cyanotoxins detection. Bot Lith 18(1):58–65
Msagati TA, Siame BA, Shushu DD (2006) Evaluation of methods for the isolation, detection and quantification of cyanobacterial hepatotoxins. Aquat Toxicol 78(4):382–397
Singh S, Srivastava A, Oh HM, Ahn CY, Choi GG, Asthana RK (2012) Recent trends in development of biosensors for detection of microcystin. Toxicon 60(5):878–894
Sivonen K, Carmichael WW, Namikoshi M, Rinehart KL, Dahlem AM, Niemelä SI (1990) Isolation and characterization of hepatotoxic microcystin homologs from the filamentous freshwater cyanobacterium Nostoc sp. strain 152. Appl Environ Microbiol 56(9):2650–2657
Skulberg OM, Carmichael WW, Codd GA, Skulberg R (1993) Taxonomy of toxic Cyanophyceae (cyanobacteria). In: Algal toxins in seafood and drinking water, pp 145–164
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Varshney, S.R., Misbah Rehman, V.Z., Ravi, L. (2023). In Vitro Assay for Determining Cyanotoxin Using Serological Methods. In: Thajuddin, N., Sankara narayanan, A., Dhanasekaran, D. (eds) Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin . Springer, Singapore. https://doi.org/10.1007/978-981-99-4514-6_34
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DOI: https://doi.org/10.1007/978-981-99-4514-6_34
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