In Vitro Assay for Determining Cyanotoxin Using Serological Methods

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Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin

Abstract

Cyanobacteria are free living organisms and the probability of finding them in water body is very high. These are proven to be harmful for humans in the form of hepatotoxins, neurotoxins, or alkaloids (forms of cyanotoxins). Thus, detection of these harmful agents becomes a necessity for a healthy environment at all forms of life. This chapter mainly focuses on the serologic methods of detecting cyanotoxins in blood sera using enzyme-linked immunosorbent assay. Although several other methods under analytical tests like chromatographic techniques can be employed to know the accurate concentration, ELISA on the other hand also furnishes good quality results. The following content will describe the protocol of performing experiments to detect cyanotoxins in their classified form.

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Varshney, S.R., Misbah Rehman, V.Z., Ravi, L. (2023). In Vitro Assay for Determining Cyanotoxin Using Serological Methods. In: Thajuddin, N., Sankara narayanan, A., Dhanasekaran, D. (eds) Protocols for Cyanobacteria Sampling and Detection of Cyanotoxin . Springer, Singapore. https://doi.org/10.1007/978-981-99-4514-6_34

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