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Reuterin: A Broad Spectrum Antimicrobial Agent and Its Applications

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Industrial Microbiology and Biotechnology

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Abstract

Probiotic bacteria play a vital role in living animals including human health by hel** in the digestion of foods and boosting the immune system. Hence, microbes with better properties are of interest. Some probiotic bacteria produce specific substances that can inhibit or inactivate other organisms which compete for nutrients and space. The β-hydroxypropionaldehyde (3-HPA) known as reuterin is produced by Lactobacillus reuteri. It has broad antimicrobial activity against potentially harmful microorganisms without adversely affecting the beneficial gut flora. Its production occurs under the anaerobic condition where the enzyme glycerol dehydratase facilitates the conversion of glycerol to reuterin by removing water molecules. The structure of reuterin contains both hydroxy and aldehyde functional groups. Reuterin is a low molecular weight water-soluble compound resilient to proteolytic and lipolytic enzymes, and remains active at a varied pH and hence finds applications in industries. This chapter focuses on reuterin production, stability, antimicrobial activity, and its applications.

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Abbreviations

1,3-PDO:

1,3-propanediol

3-HP:

3-hydroxypropionic acid

3-HPA:

3-Hydroxypropionaldehyde

FT-IR:

Fourier-transform infrared spectroscopy

GRAS:

Generally regarded as safe

HCA:

Hydroxycinnamic acid

HHP:

High hydrostatic pressure

HPLC:

High-performance liquid chromatography

IC-PAD:

Ion chromatography-pulsed amperometric detection

MRS:

De Man, Rogosa and Sharpe Medium

RP-HPLC:

Reverse phase high-performance liquid chromatography

TVC:

Total viable counts

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Acknowledgments

The authors would like to thank Rajiv Gandhi Science & Technology Commission, Mumbai, for financial support and the FIST grant of DST, New Delhi, for infrastructural facilities provided to the School of Life Sciences and School of Chemical Sciences of KBCNMU.

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Authors and Affiliations

  1. School of Life Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, India

    Kiran S. Dalal & Bhushan L. Chaudhari

  2. Department of Microbiology and Biotechnology, R. C. Patel Arts, Commerce and Science College, Shirpur, Maharashtra, India

    Sandip P. Patil

  3. Department of Microbiology, Sadguru Gadage Maharaj College, Karad, Maharashtra, India

    Girish B. Pendharkar

  4. School of Chemical Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, Maharashtra, India

    Dipak S. Dalal

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  1. Kiran S. Dalal
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  2. Sandip P. Patil
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  3. Girish B. Pendharkar
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  4. Dipak S. Dalal
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  5. Bhushan L. Chaudhari
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Correspondence to Bhushan L. Chaudhari .

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  1. Bioprocess and Bioenergy Laboratory, Department of Microbiology, Central University of Rajasthan, Kishangarh, Rajasthan, India

    Pradeep Verma

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Dalal, K.S., Patil, S.P., Pendharkar, G.B., Dalal, D.S., Chaudhari, B.L. (2023). Reuterin: A Broad Spectrum Antimicrobial Agent and Its Applications. In: Verma, P. (eds) Industrial Microbiology and Biotechnology. Springer, Singapore. https://doi.org/10.1007/978-981-99-2816-3_20

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