Abstract
Freshwater, a scarce natural source, requires conservation, and considering the rise in environmental pollution, there is a demand for advanced, sustainable, and eco-friendly wastewater reclamation technologies. Effluents coming out from different sources like livestock, food processing, or agricultural runoff contain a significant amount of nutrients, valuable bioactive compounds, and high organic load (i.e., BOD, TDS, and oily compounds). Reclamation of such wastewaters along with nutrient recovery is essential to ensure safe environmental disposal and minimize nutrient loss. Cultivating microalgae in wastewater, one of the newly explored low-cost bioremediation processes, shows high potential in this regard. In microalgal-based wastewater treatment, nutrient uptake (i.e., phosphorus, nitrogen, etc.) not only reduces the load, but also produces different valuable bioactive compounds via microalgal cultivation. However, depending on the nutrient availability, physicochemical characteristics of wastewater, adaptability and robustness of the microalgal strain, designing an optimized low-cost high-efficiency process treatment should be researched further. This chapter highlights the advantages of the microalgal-based wastewater remediation over conventional techniques, in real-time application. The article also focuses on various factors influencing microalgal growth cultivated in wastewater and the valorization of the produced microalgal biomass. Lastly, major challenges and shortcomings in using microalgal wastewater remediation at the industrial level are analyzed, along with the scope for further developments.
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References
Acién FG, Fernández JM, Magán JJ, Molina E (2012) Production cost of a real microalgae production plant and strategies to reduce it. Biotechnol Adv 30(6):1344–1353. https://doi.org/10.1016/j.biotechadv.2012.02.005
Acién FG, Gómez-Serrano C, Morales-Amaral MM, Fernández-Sevilla JM, Molina-Grima E (2016) Wastewater treatment using microalgae: how realistic a contribution might it be to significant urban wastewater treatment? Appl Microbiol Biotechnol 100(21):9013–9022. https://doi.org/10.1007/s00253-016-7835-7
Adesanya VO, Cadena E, Scott SA, Smith AG (2014) cultivation system. Bioresour Technol 163:343–355. https://doi.org/10.1016/j.biortech.2014.04.051
Aketo T, Hoshikawa Y, Nojima D, Yabu Y, Maeda Y, Yoshino T, Takano H, Tanaka T (2020) Selection and characterization of microalgae with potential for nutrient removal from municipal wastewater and simultaneous lipid production. J Biosci Bioeng 129(5):565–572. https://doi.org/10.1016/j.jbiosc.2019.12.004
Amin FR, Huang Y, He Y, Zhang R, Liu G, Chen C (2016) Biochar applications and modern techniques for characterization. Clean Technol Environ Policy 18(5):1457–1473. https://doi.org/10.1007/s10098-016-1218-8
Arora N, Patel A, Sartaj K, Pruthi PA, Pruthi V (2016) Bioremediation of domestic and industrial wastewaters integrated with enhanced biodiesel production using novel oleaginous microalgae. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-016-7320-y
Aslam A, Khan SJ, Shahzad A (2022) Science of the total environment Anaerobic membrane bioreactors (AnMBRs) for municipal wastewater treatment-potential benefits, constraints, and future perspectives: an updated review. Sci Total Environ 802:149612. https://doi.org/10.1016/j.scitotenv.2021.149612
Baglieri A, Sidella S, Barone V, Fragalà F, Silkina A, Nègre M, Gennari M (2016) Cultivating Chlorella vulgaris and Scenedesmus quadricauda microalgae to degrade inorganic compounds and pesticides in water. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-016-6996-3
Bai X, Acharya K (2016) Removal of trimethoprim, sulfamethoxazole, and triclosan by the green alga Nannochloris sp. J Hazard Mater 315:70–75. https://doi.org/10.1016/j.jhazmat.2016.04.067
Bigogno C, Khozin-Goldberg I, Adlerstein D, Cohen Z (2002) Biosynthesis of arachidonic acid in the oleaginous microalga Parietochloris incisa (chlorophyceae): radiolabeling studies. Lipids 37(2):209–216. https://doi.org/10.1007/s11745-002-0882-6
Brennan L, Owende P (2010) Biofuels from microalgae—A review of technologies for production, processing, and extractions of biofuels and co-products. Renew Sustain Energy Rev 14:557–577. https://doi.org/10.1016/j.rser.2009.10.009
Bridgwater AV (2011) Review of fast pyrolysis of biomass and product upgrading. Biomass Bioenerg 38:68–94. https://doi.org/10.1016/j.biombioe.2011.01.048
Cai T, Park SY, Li Y (2013) Nutrient recovery from wastewater streams by microalgae: status and prospects. Renew Sustain Energy Rev 19:360–369. https://doi.org/10.1016/j.rser.2012.11.030
Cavieres L, Bazaes J, Marticorena P, Riveros K, Medina P, Sepúlveda C, Riquelme C (2021) Pilot-scale phycoremediation using Muriellopsis sp. For wastewater reclamation in the Atacama Desert: microalgae biomass production and pigment recovery. Water Sci Technol 83(2):331–343. https://doi.org/10.2166/wst.2020.576
Chang Y, Tsai W, Li M (2014) Chemical characterization of char derived from slow pyrolysis of microalgal residue. J Anal Appl Pyrol, 8–13. https://doi.org/10.1016/j.jaap.2014.12.004
Chittora D, Meena M, Barupal T, Swapnil P (2020) Cyanobacteria as a source of biofertilizers for sustainable agriculture. Biochem Biophys Rep, 22 (November 2019). https://doi.org/10.1016/j.bbrep.2020.100737
Chu HQ, Tan XB, Zhang YL, Yang LB, Zhao FC, Guo J (2015) Continuous cultivation of Chlorella pyrenoidosa using anaerobic digested starch processing wastewater in the outdoors. Biores Technol 185:40–48. https://doi.org/10.1016/j.biortech.2015.02.030
Coppens J, Grunert O, Van Den Hende S, Vanhoutte I, Boon N, Haesaert G, De Gelder L (2016) The use of microalgae as a high-value organic slow-release fertilizer results in tomatoes with increased carotenoid and sugar levels. J Appl Phycol 28(4):2367–2377. https://doi.org/10.1007/s10811-015-0775-2
Das S, Nath K, Chowdhury R (2021) Comparative studies on biomass productivity and lipid content of a novel blue-green algae during autotrophic and heterotrophic growth. Environ Sci Pollut Res 28:12107–12118. https://doi.org/10.1007/s11356-020-09577-4
Ding J, Zhao F, Cao Y, **ng L, Liu W, Mei S, Li S (2015) Cultivation of microalgae in dairy farm wastewater without sterilization. Int J Phytorem 17(3):222–227. https://doi.org/10.1080/15226514.2013.876970
Ebeling JM, Timmons MB, Bisogni JJ (2006) Engineering analysis of the stoichiometry of photoautotrophic, autotrophic, and heterotrophic removal of ammonia-nitrogen in aquaculture systems. Aquaculture 257(1–4):346–358. https://doi.org/10.1016/j.aquaculture.2006.03.019
El-kassas HY, Mohamed LA (2014) Bioremediation of the textile waste effluent by Chlorella vulgaris. Egypt J Aquat Res 40(3):301–308. https://doi.org/10.1016/j.ejar.2014.08.003
Fergola P, Cerasuolo M, Pollio A, Pinto G, DellaGreca M (2007) Allelopathy and competition between Chlorella vulgaris and Pseudokirchneriella subcapitata: experiments and mathematical model. Ecol Model 208(2–4):205–214. https://doi.org/10.1016/j.ecolmodel.2007.05.024
Fernández FGA, Gómez-Serrano C, Fernández-Sevilla JM (2018) Recovery of nutrients from wastewaters using microalgae. Front Sustain Food Syst 2(September):1–13. https://doi.org/10.3389/fsufs.2018.00059
Gao B, Liu J, Zhang C, Van De Waal DB (2018) Biological stoichiometry of oleaginous microalgal lipid synthesis: the role of N: P supply ratios and growth rate on microalgal elemental and biochemical composition. Algal Res 32(April):353–361. https://doi.org/10.1016/j.algal.2018.04.019
García-Gonzáleza M, Morenoa J, Manzano JC, Florencio FJ, Guerrero GM (2005) Production of Dunaliella salina biomass rich in 9-cis-β-carotene and lutein in a closed tubular photobioreactor. J Biotechnol 115(1):81–90
Gentili FG (2014) Bioresource Technology Microalgal biomass and lipid production in mixed municipal, dairy, pulp and paper wastewater together with added flue gases. Biores Technol 169:27–32. https://doi.org/10.1016/j.biortech.2014.06.061
Guil-Guerrero JL (2007) Stearidonic acid (18:4n–3): metabolism, nutritional importance, medical uses and natural sources. Eur J Lipid Sci Technol 109(12):1226–1236. https://doi.org/10.1002/ejlt.200700207
Hejazi MA, Wijffels HR (2004) Milking of microalgae.pdf. Trends Biotechnol 22(4):189–194.
Hernández D, Riaño B, Coca M, Solana M, Bertucco A, García-González MC (2016) Microalgae cultivation in high rate algal ponds using slaughterhouse wastewater for biofuel applications. Chem Eng J 285:449–458. https://doi.org/10.1016/j.cej.2015.09.072
Kamalanathan M, Chaisutyakorn P, Gleadow R, Beardall J (2018) A comparison of photoautotrophic, heterotrophic, and mixotrophic growth for biomass production by the green alga Scenedesmus sp. (Chlorophyceae). Phycologia 57(3):309–317. https://doi.org/10.2216/17-82.1
Koreiviene J, Valčiukas R, Karosiene J, Baltrenas P (2014) Testing of Chlorella/Scenedesmus microalgae consortia for remediation of wastewater, CO2 mitigation and algae biomass feasibility for lipid production. J Environ Eng Landsc Manag 22(2):105–114. https://doi.org/10.3846/16486897.2013.911182
Kothari R, Prasad R, Kumar V, Singh DP (2013) Production of biodiesel from microalgae Chlamydomonas polypyrenoideum grown on dairy industry wastewater. Bioresour Technol 144(October 2018):499–503. https://doi.org/10.1016/j.biortech.2013.06.116
Krishnamoorthy N, Unpaprom Y, Ramaraj R, Maniam GP, Govindan N, Arunachalam T, Paramasivan B (2021) Recent advances and future prospects of electrochemical processes for microalgae harvesting. J Environ Chem Eng 9(5):105875
Küçük K, Tevatia R, Sorgüven E, Demirel Y, Özilgen M (2015) Bioenergetics of growth and lipid production in Chlamydomonas reinhardtii. Energy 83:503–510. https://doi.org/10.1016/j.energy.2015.02.054
Kurade MB, Kim JR, Govindwar SP, Jeon BH (2016) Insights into microalgae mediated biodegradation of diazinon by Chlorella vulgaris: microalgal tolerance to xenobiotic pollutants and metabolism. Algal Res 20:126–134. https://doi.org/10.1016/j.algal.2016.10.003
Lee CS, Lee SA, Ko SR, Oh HM, Ahn CY (2015) Effects of photoperiod on nutrient removal, biomass production, and algal-bacterial population dynamics in lab-scale photobioreactors treating municipal wastewater. Water Res 68:680–691. https://doi.org/10.1016/j.watres.2014.10.029
Lee XJ, Ong HC, Gan YY, Chend W-H, Mahlia TMI (2020) State of art review on conventional and advanced pyrolysis of macroalgae and microalgae for biochar, bio-oil and bio-syngas production. Energy Convers Manage 210(March):112707. https://doi.org/10.1016/j.enconman.2020.112707
Li Y, Tsai W, Hsu Y, **e M, Chen J (2014) Comparison of autotrophic and mixotrophic cultivation of green microalgal for biodiesel production. Energy Procedia 52:371–376. https://doi.org/10.1016/j.egypro.2014.07.088
Li D, Liu R, Cui X, He M, Zheng S, Du W, Gao M, Wang C (2021) Co-culture of bacteria and microalgae for treatment of high concentration biogas slurry. J Water Process Eng 41(March):102014. https://doi.org/10.1016/j.jwpe.2021.102014
Li S, Ji L, Chen C, Zhao S, Sun M, Gao Z, Wu H, Fan J (2020) Bioresource technology efficient accumulation of high-value bioactive substances by carbon to nitrogen ratio regulation in marine microalgae Porphyridium purpureum. Bioresource Technol 309(February), 123362
Lin Y, Ge J, Zhang Y, Ling H, Yan X, ** W (2019) Monoraphidium sp. HDMA-20 is a new potential source of α-linolenic acid and eicosatetraenoic acid. Lipids Health Dis 18(1):1–10. https://doi.org/10.1186/s12944-019-0996-5
Liu R, Guo Q, Zheng W, Chen L, Luo J (2015) Cultivation of an Arthrospira platensis with digested piggery wastewater. Water Sci Technol 72(10):1774–1779. https://doi.org/10.2166/wst.2015.353
Mandal S, Mallick N (2011) Waste utilization and biodiesel production by the green microalga Scenedesmus obliquus. Appl Environ Microbiol 77(1):374–377. https://doi.org/10.1128/AEM.01205-10
Marbelia L, Bilad MR, Passaris I, Discart V, Vandamme D, Beuckels A, Muylaert K, Vankelecom IFJ (2014) Membrane photobioreactors for integrated microalgae cultivation and nutrient remediation of membrane bioreactors effluent. Bioresour Technol J 163:228–235. https://doi.org/10.1016/j.biortech.2014.04.012
Marjakangas JM, Chen CY, Lakaniemi AM, Puhakka JA, Whang LM, Chang JS (2015) Simultaneous nutrient removal and lipid production with Chlorella vulgaris on sterilized and non-sterilized anaerobically pretreated piggery wastewater. Biochem Eng J 103:177–184. https://doi.org/10.1016/j.bej.2015.07.011
McDowell D, Dick JT, Eagling L, Julius M, Sheldrake GN, Theodoridou K, Walsh PJ (2020) Recycling nutrients from anaerobic digestates for the cultivation of Phaeodactylum tricornutum: a feasibility study. Algal Res 48(March):101893. https://doi.org/10.1016/j.algal.2020.101893
Mukherjee C, Chowdhury R, Sutradhar T, Begam M, Magdalene S, Kumar S, Ray K (2016) Parboiled rice effluent: a wastewater niche for microalgae and cyanobacteria with growth coupled to comprehensive remediation and phosphorus biofertilization. ALGAL 19:225–236. https://doi.org/10.1016/j.algal.2016.09.009
Muradov N, Taha M, Miranda AF, Wrede D, Kadali K, Gujar A, Stevenson T, Ball AS, Mouradov A (2015) Fungal-assisted algal flocculation: application in wastewater treatment and biofuel production. Biotechnol Biofuels 8(24). https://doi.org/10.1186/s13068-015-0210-6
Narala RR, Garg S, Sharma KK, Thomas-hall SR (2016) Comparison of microalgae cultivation in photobioreactor open raceway pond, and a two-stage hybrid system. Front Energy Res 4(August):1–10. https://doi.org/10.3389/fenrg.2016.00029
Nzayisenga JC, Farge X, Groll SL, Sellstedt A (2020) Effects of light intensity on growth and lipid production in microalgae grown in wastewater. Biotechnol Biofuels 13(1):1–8. https://doi.org/10.1186/s13068-019-1646-x
Panda S, Mishra S, Akcil A, Kucuker MA (2021) Microalgal potential for nutrient-energy-wastewater nexus: innovations, current trends and future directions. Energy Environ 32(4):604–634. https://doi.org/10.1177/0958305X20955187
Park JBK, Craggs RJ (2010) Wastewater treatment and algal production in high rate algal ponds with carbon dioxide addition. Water Sci Technol 61(3):633–639. https://doi.org/10.2166/wst.2010.951
Park JBK, Craggs RJ, Shilton AN (2013) Enhancing biomass energy yield from pilot-scale high rate algal ponds with recycling. Water Res 47(13):4422–4432. https://doi.org/10.1016/j.watres.2013.04.001
Pham TL, Bui MH (2020) Removal of nutrients from fertilizer plant wastewater using Scenedesmus sp.: formation of bioflocculation and enhancement of removal efficiency. J Chem. https://doi.org/10.1155/2020/8094272
Posadas E, del Mar Morales M, Gomez C, Acién FG, Muñoz R (2015) Influence of pH and CO2 source on the performance of microalgae-based secondary domestic wastewater treatment in outdoors pilot raceways. Chem Eng J 265:239–248. https://doi.org/10.1016/j.cej.2014.12.059
Powell N, Shilton A, Chisti Y, Pratt S (2009) Towards a luxury uptake process via microalgae—defining the polyphosphate dynamics. Water Res 43(17):4207–4213. https://doi.org/10.1016/j.watres.2009.06.011
Renuka N, Ratha SK, Kader F, Rawat I, Bux F (2021) Insights into the potential impact of algae-mediated wastewater beneficiation for the circular bioeconomy: a global perspective. J Environ Manage 297:113257. https://doi.org/10.1016/j.jenvman.2021.113257
Sajadian SF, Morowvat MH, Ghasemi Y (2018) Investigation of autotrophic, heterotrophic, and mixotrophic modes of cultivation on lipid and biomass production in Chlorella vulgaris. Nat J Physiol Pharm Pharmacol 8(4):594–599. https://doi.org/10.5455/njppp.2018.8.0935625122017
Shah MP (2020) Microbial Bioremediation & Biodegradation. Springer
Shah MP (2021a) Removal of refractory pollutants from wastewater treatment plants. CRC Press
Shah MP (2021b) Removal of emerging contaminants through microbial processes. Springer
Silambarasan S, Logeswari P, Sivaramakrishnan R, Incharoensakdi A, Cornejo P, Kamaraj B, Chi NTL (2021) Removal of nutrients from domestic wastewater by microalgae coupled to lipid augmentation for biodiesel production and influence of deoiled algal biomass as biofertilizer for Solanum lycopersicum cultivation. Chemosphere 268:129323. https://doi.org/10.1016/j.chemosphere.2020.129323
Singh D, Puri M, Wilkens S, Mathur AS, Tuli DK, Barrow CJ (2013) Characterization of a new zeaxanthin producing strain of Chlorella saccharophila isolated from New Zealand marine waters. Biores Technol 143:308–314. https://doi.org/10.1016/j.biortech.2013.06.006
Sirisuk P, Ra CH, Jeong GT, Kim SK (2018) Effects of wavelength mixing ratio and photoperiod on microalgal biomass and lipid production in a two-phase culture system using LED illumination. Bioresour Technol 253:175–181. https://doi.org/10.1016/j.biortech.2018.01.020
Solovchenko A, Verschoor AM, Jablonowski ND, Nedbal L (2016) Phosphorus from wastewater to crops: an alternative path involving microalgae. Biotechnol Adv 34(5):550–564. https://doi.org/10.1016/j.biotechadv.2016.01.002
Solovchenko AE, Ismagulova TT, Lukyanov AA, Vasilieva SG, Konyukhov IV, Pogosyan SI, Lobakova ES, Gorelova OA (2019) Luxury phosphorus uptake in microalgae. J Appl Phycol 31(5):2755–2770. https://doi.org/10.1007/s10811-019-01831-8
Sriram S, Seenivasan R (2012) Microalgae cultivation in wastewater for nutrient removal. J Algal Biomass Utilization 3(2):9–13
Stephenson AL, Kazamia E, Dennis JS, Howe CJ, Scott SA, Smith AG (2010) Life-cycle assessment of potential algal biodiesel production in the United Kingdom: a comparison of raceways and air-lift tubular bioreactors. Energy Fuels 4:4062–4077. https://doi.org/10.1021/ef1003123
Supraja KV, Behera B, Balasubramanian P (2020) Efficacy of microalgal extracts as biostimulants through seed treatment and foliar spray for tomato cultivation. Ind Crops Prod 151(March):112453. https://doi.org/10.1016/j.indcrop.2020.112453
Tatarová D, Galanda D, Kuruc J, Gaálová B (2021) Phytoremediation of 137Cs, 60Co, 241Am, and 239Pu from aquatic solutions using Chlamydomonas reinhardtii, Scenedesmus obliquus, and Chlorella vulgaris. Int J Phytorem 23(13):1376–1381. https://doi.org/10.1080/15226514.2021.1900061
Torri C, Samorì C, Adamiano A, Fabbri D, Faraloni C, Torzillo G (2011) Preliminary investigation on the production of fuels and bio-char from Chlamydomonas reinhardtii biomass residue after bio-hydrogen production. Biores Technol 102(18):8707–8713. https://doi.org/10.1016/j.biortech.2011.01.064
Ward OP, Singh A (2005) Omega-3/6 fatty acids: alternative sources of production. Process Biochem 40(12):3627–3652. https://doi.org/10.1016/j.procbio.2005.02.020
Whitton R, Ometto F, Pidou M, Jarvis P, Villa R, Jefferson B (2015) Microalgae for municipal wastewater nutrient remediation: mechanisms, reactors and outlook for tertiary treatment. Environ Technol Rev 4(1):133–148. https://doi.org/10.1080/21622515.2015.1105308
Xu J, Wang X, Sun S, Zhao Y, Hu C (2017) Effects of influent C/N ratios and treatment technologies on integral biogas upgrading and pollutants removal from synthetic domestic sewage, pp 1–13. https://doi.org/10.1038/s41598-017-11207-y
Yang J, Cao J, **ng G, Yuan H (2015) Bioresource Technology Lipid production combined with biosorption and bioaccumulation of cadmium, copper, manganese and zinc by oleaginous microalgae Chlorella minutissima UTEX2341. Biores Technol 175:537–544. https://doi.org/10.1016/j.biortech.2014.10.124
Yao C, Wu P, Pan Y, Lu H, Chi L, Meng Y, Cao X, Xue S, Yang X (2016) Bioresource technology evaluation of the integrated hydrothermal carbonization-algal cultivation process for enhanced nitrogen utilization in Arthrospira platensis production. Biores Technol 216:381–390. https://doi.org/10.1016/j.biortech.2016.05.110
Yu KL, Show PL, Ong HC, Ling TC, Chen WH, Salleh MAM (2018) Biochar production from microalgae cultivation through pyrolysis as a sustainable carbon sequestration and biorefinery approach. Clean Technol Environ Policy 20(9):2047–2055. https://doi.org/10.1007/s10098-018-1521-7
Zheng H, Liu M, Lu Q, Wu X, Ma Y, Cheng Y, Addy M, Liu Y, Ruan R (2017) Balancing carbon/nitrogen ratio to improve nutrients removal and algal biomass production in piggery and brewery wastewaters. Biores Technol. https://doi.org/10.1016/j.biortech.2017.10.057
Zheng Q, Xu X, Martin GJO, Kentish SE (2018) Critical review of strategies for CO2 delivery to large-scale microalgae cultures. Chin J Chem Eng 26(11):2219–2228. https://doi.org/10.1016/j.cjche.2018.07.013
Zhu L, Hu T, Li S, Nugroho YK, Li B, Cao J, Show P-L, Hiltunen E (2020) Effects of operating parameters on algae Chlorella vulgaris biomass harvesting and lipid extraction using metal sulfates as flocculants. Biomass Bioenergy 132:105433
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The authors thank the Department of Biotechnology and Medical Engineering of National Institute of Technology Rourkela for providing the necessary research facility. The authors greatly acknowledge the Ministry of Science and Technology, Government of India, for sponsoring the Ph.D. program of the first author.
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Chaudhuri, R., Krishnamoorthy, N., Paramasivan, B. (2023). Role of Microalgae in Integrated Wastewater Remediation and Valorization of Value-Added Compounds. In: Shah, M.P. (eds) Sustainable Industrial Wastewater Treatment and Pollution Control. Springer, Singapore. https://doi.org/10.1007/978-981-99-2560-5_4
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