Abstract
During the last decades, the field of stem cell research has rapidly expanded with the potential to develop the technology of tissue regeneration as well as the discovery of novel therapeutic agents. The innovative procedure of cellular reprogramming, which was found by Yamanaka in 2007, lead to the generation of induced pluripotent stem cells (iPSCs) from a somatic cell namely a normal fibroblast. The maintenance of iPSCs in a successful manner is critical issue because undifferentiated state should be kept in the culture. Especially, extra attention should be payed to maintain their key characteristics of self-renewal and pluripotency avoiding unexpected differentiation. In this chapter, we describe the basic techniques necessary to culture human or mouse iPSCs, e.g starting from the frozen stocks, eeding cells into culture vessels, changing media, passaging, and cryopreservation.
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References
Bioani M, Schöler HR. Regulatory networks in embryo-derived pluripotent stem cells. Nat Rev Mol Cell Biol. 2006;6:872–84.
Boyer L, Plath K, Zeitlinger J, Brambrink T, Medeiros LA, Lee TI, Levine SS, Wernig M, Tajonar A, Ray MK, et al. Polycomb complexes repress developmental regulators in murine embryonic stem cells. Nature. 2006;441:349–53.
Ding VM, Ling L, Natarajan S, Yap MG, Cool SM, Choo AB. FGF-2 modulates Wnt signaling in undifferentiated hESC and iPS cells through activated PI3-K/GSK3beta signaling. J Cell Physiol. 2010;225:417–28.
Matsuda T, Nakamura T, Nakao K, Arai K, Katsuki M, Heike T, Yokota T. STAT3 activation is sufficient to maintain an undifferentiated state of mouse embryonic stem cells. EMBO J. 1999;18:4261–9.
Nakashima Y, Omasa T. What Kind of Signaling Maintains Pluripotency and Viability in Human-Induced Pluripotent Stem Cells Cultured on Laminin-511 with Serum-Free Medium? Biores Open Access. 2016;5(1):84-93.
Niwa H, Burdon T, Chambers I, Smith A. Self-renewal of pluripotent embryonic stem cells is mediated via activation of STAT3. Genes Dev. 1998;12:2048–60.
Niwa H, Miyazaki J, Smith AG. Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat Genet. 2000;24:372–6.
Solter D. From teratocarcinomas to embryonic stem cells and beyond: a history of embryonic stem cell research. Nat Rev Genet. 2006;7:319–27.
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006;126(4):663–76. https://doi.org/10.1016/j.cell.2006.07.024. Epub 2006 Aug 10. PMID: 16904174.
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Afify, S.M., Seno, M. (2023). Maintenance of Human Pluripotent Stem Cells. In: Methods in Cancer Stem Cell Biology. Springer, Singapore. https://doi.org/10.1007/978-981-99-1331-2_5
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DOI: https://doi.org/10.1007/978-981-99-1331-2_5
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