Abstract
GPCR immobilization is fundamental for many bioanalytical methods, which are crucial for receptor-ligand interaction analysis and drug discovery. A desirable way to immobilize GPCRs on solid surfaces is oriented immobilization. In this way, the receptors can be optimal in their orientation, conformation, and activity or functional studies. In this chapter, we review the affinity-based non-covalent and site-specific covalent immobilization methods developed for capturing GPCR on solid surfaces. The cons and pros of each method are also discussed.
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Abbreviations
- GPCRs:
-
G protein-coupled receptors
- NHS:
-
N-Hydroxysuccinimide
- 5-HT3R:
-
5-Hydroxytryptamlne receptor
- nAChR:
-
Nicotinic acetylcholine receptor
- EDTA:
-
Ethylenediaminetetraacetic acid
- NTS-1:
-
Neurotensin receptor-1
- β2-AR:
-
β2-Adrenergic receptor
- ssDNA:
-
Single-stranded DNA
- EGFR:
-
Epidermal growth factor receptor
- SrtA:
-
Sortase A
- hAGT:
-
O6-Alkylguanine-DNA alkyltransferase
- BG:
-
O6-Benzylguanine
- Halo:
-
Haloalkane dehalogenase
- EPL:
-
Expressed protein ligation
- MNPs:
-
Magnetic nanoparticles
- CalBP:
-
Calmodulin-binding peptide
- CBD:
-
Cellulose-binding domains
- MBP:
-
Maltose-binding protein
- Im7:
-
Immunity protein 7
- GST:
-
Glutathione S-transferase
- Pr-A:
-
Protein A
- HA:
-
Hemagglutinin A
- VSV-G:
-
Vesicular stomatitis virus glycoprotein
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Zhao, X., Li, Q., Wang, J., Liang, Q., Quan, J. (2023). Oriented Immobilization of G Protein-Coupled Receptors. In: G Protein-Coupled Receptors . SpringerBriefs in Molecular Science. Springer, Singapore. https://doi.org/10.1007/978-981-99-0078-7_3
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