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Semiconductor Nanocrystals for Biological Imaging and Fluorescence Spectroscopy

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Advanced Imaging and Bio Techniques for Convergence Science

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1310))

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Abstract

Semiconductor nanocrystals (SNCs) are a nano-sized inorganic material. Due to the quantum confinement effect, these crystals exhibit unique optical and electrical properties. This chapter focuses on biological applications of SNCs, ranging from in vitro single-molecule tracking to in vivo fluorescence imaging. The following fundamental properties and technical procedures related to SNCs are also described: structures of SNCs, synthetic procedures and shape control of SNCs, preparation methods of water-soluble SNCs, and conjugation methods of biomolecules.

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References

  • Abbandonato G, Hoffmann K, Resch-Genger U (2018) Determination of quantum yields of semiconductor nanocrystals at the single emitter level via fluorescence correlation spectroscopy. Nanoscale 10(15):7147–7154

    Article  CAS  PubMed  Google Scholar 

  • Adachi K, Yasuda R, Noji H, Itoh H, Harada Y, Yoshida M, Kinosita K Jr (2000) Step** rotation of F1-ATPase visualized through angle-resolved single-fluorophore imaging. Proc Natl Acad Sci U S A 97(13):7243–7247

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Alivisatos AP (1996) Semiconductor clusters, nanocrystals, and quantum dots. Science 271(5251):933–937

    Article  CAS  Google Scholar 

  • Bale G, Elwell CE, Tachtsidis I (2016) From Jöbsis to the present day: a review of clinical near-infrared spectroscopy measurements of cerebral cytochrome-c-oxidase. J Biomed Opt 21(9):091307

    Article  PubMed  Google Scholar 

  • Baranov AV, Rakovich YP, Donegan JF, Perova TS, Moore RA, Talapin DV, Rogach AL, Masumoto Y, Nabiev I (2003) Effect of ZnS shell thickness on the phonon spectra in CdSe quantum dots. Phys Rev B 68(16):165306–165306-7

    Article  CAS  Google Scholar 

  • Bayer M (2019) Bridging two worlds: colloidal versus epitaxial quantum dots. Ann Phys (Berlin) 531:1900039

    Article  CAS  Google Scholar 

  • Beauvoit B, Chance B (1998) Time-resolved spectroscopy of mitochondria, cells and tissues under normal and pathological conditions. Mol Cell Biochem 184(1-2):445–455

    Article  CAS  PubMed  Google Scholar 

  • Bell AG (1880) Upon the production and reproduction of sound by light. Am J Sci 20:305–324

    Article  Google Scholar 

  • Benson RC, Kues HA (1978) Fluorescence properties of indocyanine green as related to angiography. Phys Med Biol 23(1):159–163

    Article  CAS  PubMed  Google Scholar 

  • Bera D, Qian L, Tseng TK, Holloway PH (2010) Quantum dots and their multimodal applications: a review. Materials 3(4):2260–2345

    Article  CAS  PubMed Central  Google Scholar 

  • Brogan RJ, Kontojannis V, Garara B, Marcus HJ, Wilson MH (2017) Near-infrared spectroscopy (NIRS) to detect traumatic intracranial haematoma: a systematic review and meta-analysis. Brain Inj 31(5):581–588

    Article  PubMed  Google Scholar 

  • Bruchez MJR, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281(5385):2013–2016

    Article  CAS  PubMed  Google Scholar 

  • Brunnbauer M, Dombi R, Ho TH, Schliwa M, Rief M, Ökten Z (2012) Torque generation of kinesin motors is governed by the stability of the neck domain. Mol Cell 46(2):147–158

    Article  CAS  PubMed  Google Scholar 

  • Bruns OT, Bischof TS, Harris DK, Franke D, Shi Y, Riedemann L, Bartelt A, Jaworski FB, Carr JA, Rowlands CJ, Wilson MWB, Chen O, Wei H, Hwang GW, Montana DM, Coropceanu I, Achorn OB, Kloepper J, Heeren J, So PTC, Fukumura D, Jensen KF, Jain RK, Bawendi MG (2017) Next-generation in vivo optical imaging with short-wave infrared quantum dots. Nat Biomed Eng 1:0056

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Can S, Dewitt MA, Yildiz A (2014) Bidirectional helical motility of cytoplasmic dynein around microtubules. elife 3:e03205

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Cao Q, Zhegalova NG, Wang ST, Akers WJ, Berezin MY (2013) Multispectral imaging in the extended near-infrared window based on endogenous chromophores. J Biomed Opt 18(10):101318

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Chan WC, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281(5385):2016–2018

    Article  CAS  PubMed  Google Scholar 

  • Cho J, Jung YK, Lee JK, Jung HS (2017) Highly efficient blue-emitting CdSe-derived core/shell gradient alloy quantum dots with improved photoluminescent quantum yield and enhanced photostability. Langmuir 33(15):3711–3719

    Article  CAS  PubMed  Google Scholar 

  • Dabbousi BO, Rodriguezviejo J, Mikulec FV, Heine JR, Mattoussi H, Ober R, Jensen KF, Bawendi MG (1997) (CdSe)ZnS core-shell quantum dots: synthesis and characterization of a size series of highly luminescent nanocrystallites. J Phys Chem B 101:9463–9475

    Article  CAS  Google Scholar 

  • de Thomaz AA, Almeida DB, Pelegati VB, Carvalho HF, Cesar CL (2015) Measurement of the hydrodynamic radius of quantum dots by fluorescence correlationspectroscopy excluding blinking. J Phys Chem B 119(11):4294–4299

    Article  PubMed  CAS  Google Scholar 

  • Deka S, Quarta A, Lupo MG, Falqui A, Boninelli S, Giannini C, Morello G, De Giorgi M, Lanzani G, Spinella C, Cingolani R, Pellegrino T, Manna L (2009) CdSe/CdS/ZnS double shell nanorods with high photoluminescence efficiency and their exploitation as biolabeling probes. J Am Chem Soc 131(8):2948–2958

    Article  CAS  PubMed  Google Scholar 

  • Denk W, Strickler JH, Webb WW (1990) Two-photon laser scanning fluorescence microscopy. Science 248:73–76

    Article  CAS  PubMed  Google Scholar 

  • Diao S, Hong G, Antaris AL, Blackburn JL, Cheng K, Cheng Z, Dai H (2015) Biological imaging without autofluorescence in the second near-infrared region. Nano Res 8:3027–3034

    Article  CAS  Google Scholar 

  • Dong B, Li C, Chen G, Zhang Y, Zhang Y, Deng M, Wang Q (2013) Facile synthesis of highly Photoluminescent Ag2Se quantum dots as a new fluorescent probe in the second near-infrared window for in vivo imaging. Chem Mater 25:2503–2509

    Article  CAS  Google Scholar 

  • Dubertret B, Skourides P, Norris DJ, Noireaux V, Brivanlou AH, Libchaber A (2002) In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science 298(5599):1759–1762

    Article  CAS  PubMed  Google Scholar 

  • Eigen M, Rigler R (1994) Sorting single molecules: application to diagnostics and evolutionary biotechnology. Proc Natl Acad Sci U S A 91:5740–5747

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Elson EL, Magde D (1974) Fluorescence correlation spectroscopy: I. conceptual basis and theory. Biopolymers 13:1–27

    Article  CAS  Google Scholar 

  • Frangioni JV (2003) In vivo near-infrared fluorescence imaging. Curr Opin Chem Biol 7(5):626–634

    Article  CAS  PubMed  Google Scholar 

  • Fujii F, Nodasaka Y, Nishimura G, Tamura M (2004) Anoxia induces matrix shrinkage accompanied by an increase in light scattering in isolated brain mitochondria. Brain Res 999(1):29–39

    Article  CAS  PubMed  Google Scholar 

  • Fujii F, Horiuchi M, Ueno M, Sakata H, Nagao I, Tamura M, Kinjo M (2007) Detection of prion protein immune complex for bovine spongiform encephalopathy diagnosis using fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy. Anal Biochem 370(2):131–141

    Article  CAS  PubMed  Google Scholar 

  • Fujii F, Kinjo M (2007) Detection of antigen protein by using fluorescence cross-correlation spectroscopy and quantum-dot-labeled antibodies. Chembiochem 8(18):2199–2203

    Article  CAS  PubMed  Google Scholar 

  • Gao X, Cui Y, Levenson RM, Chung LW, Nie S (2004) In vivo cancer targeting and imaging with semiconductor quantum dots. Nat Biotechnol 22(8):969–976

    Article  CAS  PubMed  Google Scholar 

  • Giepmans BN, Adams SR, Ellisman MH, Tsien RY (2006) The fluorescent toolbox for assessing protein location and function. Science 312(5771):217–224

    Article  CAS  PubMed  Google Scholar 

  • Goldman ER, Anderson GP, Tran PT, Mattoussi H, Charles PT, Mauro JM (2002) Conjugation of luminescent quantum dots with antibodies using an engineered adaptor protein to provide new reagents for fluoroimmunoassays. Anal Chem 74(4):841–847

    Article  CAS  PubMed  Google Scholar 

  • Goldstein L, Glas F, Marzin JY, Charasse MN, Le Roux G (1985) Growth by molecular beam epitaxy and characterization of InAs/GaAs strained-layer superlattices. Appl Phys Lett 47(10):1099–1101

    Article  CAS  Google Scholar 

  • Golovynskyi S, Golovynska I, Stepanova LI, Datsenko OI, Liu L, Qu J, Ohulchanskyy TY (2018) Optical windows for head tissues in near-infrared and short-wave infrared regions: approaching transcranial light applications. J Biophotonics 11(12):e201800141

    Article  PubMed  Google Scholar 

  • Groeneveld E, Witteman L, Lefferts M, Ke X, Bals S, Van Tendeloo G, Donega Cde M (2013) Tailoring ZnSe-CdSe colloidal quantum dots via cation exchange: from core/shell to alloy nanocrystals. ACS Nano 7(9):7913–7930

    Article  CAS  PubMed  Google Scholar 

  • Guan LY, Li YQ, Lin S, Zhang MZ, Chen J, Ma ZY, Zhao YD (2012) Characterization of CdTe/CdSe quantum dots-transferrin fluorescent probes for cellular labeling. Anal Chim Acta 741:86–92

    Article  CAS  PubMed  Google Scholar 

  • Guo W, Qiu Z, Guo C, Ding D, Li T, Wang F, Sun J, Zheng N, Liu S (2017) Multifunctional theranostic agent of Cu2(OH)PO4 quantum dots for photoacoustic image-guided photothermal/photodynamic combination cancer therapy. ACS Appl Mater Interfaces 9(11):9348–9358

    Article  CAS  PubMed  Google Scholar 

  • Hamaoka T, McCully KK (2019) Review of early development of near-infrared spectroscopy and recent advancement of studies on muscle oxygenation and oxidative metabolism. J Physiol Sci 69(6):799–811

    Article  CAS  PubMed  Google Scholar 

  • Helmchen F, Denk W (2005) Deep tissue two-photon microscopy. Nat Methods 2(12):932–940

    Article  CAS  PubMed  Google Scholar 

  • Hong G, Robinson JT, Zhang Y, Diao S, Antaris AL, Wang Q, Dai H (2012) In vivo fluorescence imaging with Ag2S quantum dots in the second near-infrared region. Angew Chem Int Ed Eng 51(39):9818–9821

    Article  CAS  Google Scholar 

  • Hoshi Y, Yamada Y (2016) Overview of diffuse optical tomography and its clinical applications. J Biomed Opt 21(9):091312

    Article  PubMed  Google Scholar 

  • Hu J, Li LS, Yang W, Manna L, Wang L, Alivisatos AP (2001) Linearly polarized emission from colloidal semiconductor quantum rods. Science 292(5524):2060–2063

    Article  CAS  PubMed  Google Scholar 

  • Ichimura T, ** T, Fujita H, Higuchi H, Watanabe TM (2014) Nano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticles. Front Physiol 5:273

    Article  PubMed  PubMed Central  Google Scholar 

  • Jacques SL (2013) Optical properties of biological tissues: a review. Phys Med Biol 58(14):5007–5008

    Article  Google Scholar 

  • Jiang W, Mardyani S, Fischer H, Chan WCW (2006) Design and characterization of lysine cross-linked mercapto-acid biocompatible quantum dots. Chem Mater 18:872–878

    Article  CAS  Google Scholar 

  • ** T, Fujii F, Komai Y, Seki J, Seiyama A, Yoshioka Y (2008a) Preparation and characterization of highly fluorescent, glutathione-coated near infrared quantum dots for in vivo fluorescence imaging. Int J Mol Sci 9(10):2044–2061

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • ** T, Yoshioka Y, Fujii F, Komai Y, Seki J, Seiyama A (2008b) Gd3+−functionalized near-infrared quantum dots for in vivo dual modal (fluorescence/magnetic resonance) imaging. Chem Commun (Camb) 44:5764–5766

    Article  CAS  Google Scholar 

  • Jöbsis F (1977) Noninvasive, infrared monitoring of cerebral and myocardial oxygen sufficiency and circulatory parameters. Science 198(4323):1264–1267

    Article  PubMed  Google Scholar 

  • Jun YW, Lee SM, Kang NJ, Cheon J (2001) Controlled synthesis of multi-armed CdS nanorod architectures using monosurfactant system. J Am Chem Soc 123(21):5150–5151

    Article  CAS  PubMed  Google Scholar 

  • Kask P, Piksarv P, Pooga M, Mets U, Lippmaa E (1989) Separation of the rotational contribution in fluorescence correlation experiments. Biophys J 55(2):213–220

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kettling U, Koltermann A, Schwille P, Eigen M (1998) Real-time enzyme kinetics monitored by dual-color fluorescence cross-correlation spectroscopy. Proc Natl Acad Sci U S A 95(4):1416–1420

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kim S, Lim YT, Soltesz EG, De Grand AM, Lee J, Nakayama A, Parker JA, Mihaljevic T, Laurence RG, Dor DM, Cohn LH, Bawendi MG, Frangioni JV (2004) Near-infrared fluorescent type II quantum dots for sentinel lymph node map**. Nat Biotechnol 22(1):93–97

    Article  CAS  PubMed  Google Scholar 

  • Kinjo M, Rigler R (1995) Ultrasensitive hybridization analysis using fluorescence correlation spectroscopy. Nucleic Acids Res 23:1795–1799

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kirchner C, Liedl T, Kudera S, Pellegrino T, Muñoz Javier A, Gaub HE, Stölzle S, Fertig N, Parak WJ (2005) Cytotoxicity of colloidal CdSe and CdSe/ZnS nanoparticles. Nano Lett 5(2):331–338

    Article  CAS  PubMed  Google Scholar 

  • Kobayashi H, Hama Y, Koyama Y, Barrett T, Regino CAS, Urano Y, Choyke PL (2007) Simultaneous multicolor imaging of five different lymphatic basins using quantum dots. Nano Lett 7(6):1711–1716

    Article  CAS  PubMed  Google Scholar 

  • Kogure T, Karasawa S, Araki T, Saito K, Kinjo M, Miyawaki A (2006) A fluorescent variant of a protein from the stony coral Montipora facilitates dual-color single-laser fluorescence cross-correlation spectroscopy. Nat Biotechnol 24(5):577–581

    Article  CAS  PubMed  Google Scholar 

  • Koneswaran M, Narayanaswamy R (2015) Ultrasensitive detection of vitamin B6 using functionalised CdS/ZnS Core−shell quantum dots. Sensors Actuators B Chem 210:811–816

    Article  CAS  Google Scholar 

  • Kubitscheck U, Kückmann O, Kues T, Peters R (2000) Imaging and tracking of single GFP molecules in solution. Biophys J 78(4):2170–2179

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kural C, Kim H, Syed S, Goshima G, Gelfand VI, Selvin PR (2005) Kinesin and dynein move a peroxisome in vivo: a tug-of-war or coordinated movement? Science 308(5727):1469–1472

    Article  CAS  PubMed  Google Scholar 

  • Larson DR, Zipfel WR, Williams RM, Clark SW, Bruchez MP, Wise FW, Webb WW (2003) Water-soluble quantum dots for multiphoton fluorescence imaging in vivo. Science 300(5624):1434–1436

    Article  CAS  PubMed  Google Scholar 

  • Leatherdale CA, Woo WK, Mikulec FV, Bawendi MG (2002) On the absorption cross section of CdSe nanocrystal quantum dots. J Phys Chem B 106:7619–7622

    Article  CAS  Google Scholar 

  • Lee J, Fujii F, Kim SY, Pack CG, Kim SW (2014) Analysis of quantum rod diffusion by polarized fluorescence correlation spectroscopy. J Fluoresc 24(5):1371–1378

    Article  CAS  PubMed  Google Scholar 

  • Li C, Zhang Y, Wang M, Zhang Y, Chen G, Li L, Wu D, Wang Q (2014) In vivo real-time visualization of tissue blood flow and angiogenesis using Ag2S quantum dots in the NIR-II window. Biomaterials 35(1):393–400

    Article  CAS  PubMed  Google Scholar 

  • Lim YT, Kim S, Nakayama A, Stott NE, Bawendi MG, Frangioni JV (2003) Selection of quantum dot wavelengths for biomedical assays and imaging. Mol Imaging 2(1):50–64

    Article  CAS  PubMed  Google Scholar 

  • Liu W, Choi HS, Zimmer JP, Tanaka E, Frangioni JV, Bawendi M (2007) Compact cysteine-coated CdSe(ZnCdS) quantum dots for in vivo applications. J Am Chem Soc 129(47):14530–14531

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lundqvist M, Stigler J, Elia G, Lynch I, Cedervall T, Dawson KA (2008) Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts. Proc Natl Acad Sci U S A 105(38):14265–14270

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lutich AA, Mauser C, Da Como E, Huang J, Vaneski A, Talapin DV, Rogach AL, Feldmann J (2010) Multiexcitonic dual emission in CdSe/CdS tetrapods and nanorods. Nano Lett 10(11):4646–4650

    Article  CAS  PubMed  Google Scholar 

  • Magde D, Elson EL, Webb WW (1972) Thermodynamic fluctuations in a reacting system—measurement by fluorescence correlation spectroscopy. Phys Rev Lett 29:705–708

    Article  CAS  Google Scholar 

  • Magde D, Elson E, Webb WW (1974) Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers 4:29–61

    Article  Google Scholar 

  • Mahler B, Spinicelli P, Buil S, Quelin X, Hermier JP, Dubertret B (2008) Towards non-blinking colloidal quantum dots. Nat Mater 7:659–664

    Article  CAS  PubMed  Google Scholar 

  • Martinic I, Eliseeva SV, Petoud S (2017) Near-infrared emitting probes for biological imaging: organic fluorophores, quantum dots, fluorescent proteins, lanthanide(III) complexes and nanomaterials. J Lumin 189:19–43

    Article  CAS  Google Scholar 

  • Michalet X, Pinaud FF, Bentolila LA, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S (2005) Quantum dots for live cells, in vivo imaging and diagnostics. Science 307(5709):538–544

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mishra N, Wu WY, Srinivasan BM, Hariharaputran R, Zhang YW, Chan Y (2016) Continuous shape tuning of nanotetrapods: toward shape-mediated self-assembly. Chem Mater 28:1187–1195

    Article  CAS  Google Scholar 

  • Mishra N, Orfield NJ, Wang F, Hu Z, Krishnamurthy S, Malko AV, Casson JL, Htoon H, Sykora M, Hollingsworth JA (2017) Using shape to turn off blinking for two-colour multiexciton emission in CdSe/CdS tetrapods. Nat Commun 8:15083

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Monopoli MP, Aberg C, Salvati A, Dawson KA (2012) Biomolecular coronas provide the biological identity of nanosized materials. Nat Nanotechnol 7(12):779–786

    Article  CAS  PubMed  Google Scholar 

  • Murray CB, Norris DJ, Bawendi MG (1993) Synthesis and characterization of nearly monodisperse CdE (E = sulfur, selenium, tellurium) semiconductor nanocrystallites. J Am Chem Soc 115:8706–8715

    Article  CAS  Google Scholar 

  • Nachabé R, Evers DJ, Hendriks BH, Lucassen GW, van der Voort M, Rutgers EJ, Peeters MJ, Van der Hage JA, Oldenburg HS, Wesseling J, Ruers TJ (2011) Diagnosis of breast cancer using diffuse optical spectroscopy from 500 to 1600 nm: comparison of classification methods. J Biomed Opt 16(8):087010

    Article  PubMed  CAS  Google Scholar 

  • Nakane Y, Tsukasaki Y, Sakata T, Yasuda H, ** T (2013) Aqueous synthesis of glutathione-coated PbS quantum dots with tunable emission for non-invasive fluorescence imaging in the second near-infrared biological window (1000-1400 nm). Chem Commun (Camb) 49(69):7584–7586

    Article  CAS  Google Scholar 

  • Nan X, Sims PA, Chen P, **e XS (2005) Observation of individual microtubule motor steps in living cells with endocytosed quantum dots. J Phys Chem B 109(51):24220–24224

    Article  CAS  PubMed  Google Scholar 

  • Norris DJ, Bawendi MG (1996) Measurement and assignment of the size-dependent optical spectrum in CdSe quantum dots. Phys Rev B 53(24):16338–16346

    Article  CAS  Google Scholar 

  • Ohmachi M, Komori Y, Iwane AH, Fujii F, ** T, Yanagida T (2012) Fluorescence microscopy for simultaneous observation of 3D orientation and movement and its application to quantum rod-tagged myosin V. Proc Natl Acad Sci U S A 109(14):5294–5298

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Oura M, Yamamoto J, Ishikawa H, Mikuni S, Fukushima R, Kinjo M (2016) Polarization-dependent fluorescence correlation spectroscopy for studying structural properties of proteins in living cell. Sci Rep 6:31091

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Peng X, Manna L, Yang W, Wickham J, Scher E, Kadavanich A, Alivisatos AP (2000) Shape control of CdSe nanocrystals. Nature 404(6773):59–61

    Article  CAS  PubMed  Google Scholar 

  • Resch-Genger U, Grabolle M, Cavaliere-Jaricot S, Nitschke R, Nann T (2008) Quantum dots versus organic dyes as fluorescent labels. Nat Methods 5(9):763–775

    Article  CAS  PubMed  Google Scholar 

  • Rigler R, Mets U, Widengren J, Kask P (1993) Fluorescence correlation spectroscopy with high count rates and low background analysis of translational diffusion. Eur Biophys J 22:169–175

    Article  CAS  Google Scholar 

  • Sabino CP, Deana AM, Yoshimura TM, da Silva DF, França CM, Hamblin MR, Ribeiro MS (2016) The optical properties of mouse skin in the visible and near infrared spectral regions. J Photochem Photobiol B 160:72–78

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saito K, Wada I, Tamura M, Kinjo M (2004) Direct detection of caspase-3 activation in single live cells by cross-correlation analysis. Biochem Biophys Res Commun 324:849–854

    Article  CAS  PubMed  Google Scholar 

  • Schwille P, Meyer-Almes FJ, Rigler R (1997) Dual-color fluorescence cross-correlation spectroscopy for multicomponent diffusional analysis in solution. Biophys J 72(4):1878–1886

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Sen AN, Gopinath SP, Robertson CS (2016) Clinical application of near-infrared spectroscopy in patients with traumatic brain injury: a review of the progress of the field. Neurophotonics 3(3):031409

    Article  PubMed  PubMed Central  Google Scholar 

  • Shashkov EV, Everts M, Galanzha EI, Zharov VP (2008) Quantum dots as multimodal photoacoustic and photothermal contrast agents. Nano Lett 8(11):3953–3958

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Smith AM, Mohs AM, Nie S (2009) Tuning the optical and electronic properties of colloidal nanocrystals by lattice strain. Nat Nanotechnol 4(1):56–63

    Article  CAS  PubMed  Google Scholar 

  • Sugawa M, Masaike T, Mikami N, Yamaguchi S, Shibata K, Saito K, Fujii F, Toyoshima YY, Nishizaka T, Yajima J (2018) Circular orientation fluorescence emitter imaging (COFEI) of rotational motion of motor proteins. Biochem Biophys Res Commun 504(4):709–714

    Article  CAS  PubMed  Google Scholar 

  • Susumu K, Mei BC, Mattoussi H (2009) Multifunctional ligands based on dihydrolipoic acid and polyethylene glycol to promote biocompatibility of quantum dots. Nat Protoc 4(3):424–436

    Article  CAS  PubMed  Google Scholar 

  • Talapin DV, Nelson JH, Shevchenko EV, Aloni S, Sadtler B, Alivisatos AP (2007) Seeded growth of highly luminescent CdSe/CdS nanoheterostructures with rod and tetrapod morphologies. Nano Lett 7(10):2951–2959

    Article  CAS  PubMed  Google Scholar 

  • Tsay JM, Doose S, Weiss S (2006) Rotational and translational diffusion of peptide-coated CdSe/CdS/ZnS nanorods studied by fluorescence correlation spectroscopy. J Am Chem Soc 128(5):1639–1647

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang F, Wan H, Ma Z, Zhong Y, Sun Q, Tian Y, Qu L, Du H, Zhang M, Li L, Ma H, Luo J, Liang Y, Li WJ, Hong G, Liu L, Dai H (2019) Light-sheet microscopy in the near-infrared II window. Nat Methods 16(6):545–552

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Wang H, Shang L, Maffre P, Hohmann S, Kirschhöfer F, Brenner-Weiß G, Nienhaus GU (2016) The nature of a hard protein Corona forming on quantum dots exposed to human blood serum. Small 12(42):5836–5844

    Article  CAS  PubMed  Google Scholar 

  • Wang LV, Yao J (2016) A practical guide to photoacoustic tomography in the life sciences. Nat Methods 13(8):627–638

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Watanabe TM, Fujii F, ** T, Umemoto E, Miyasaka M, Fujita H, Yanagida T (2013) Four-dimensional spatial nanometry of single particles in living cells using polarized quantum rods.Biophys J 105(3):555–564

    Google Scholar 

  • Welsher K, Liu Z, Sherlock SP, Robinson JT, Chen Z, Daranciang D, Dai H (2009) A route to brightly fluorescent carbon nanotubes for near-infrared imaging in mice. Nat Nanotechnol 4(11):773–780

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Widengren J, Mets U, Rigler R (1999) Photodynamic properties of green fluorescent proteins investigated by fluorescence correlation spectroscopy. Chem Phys 250:171–186

    Article  CAS  Google Scholar 

  • Winkler T, Kettling U, Koltermann A, Eigen M (1999) Confocal fluorescence coincidence analysis: an approach to ultra high-throughput screening. Proc Natl Acad Sci U S A 96(4):1375–1378

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wong JI, Mishra N, **ng G, Li M, Chakrabortty S, Sum TC, Shi Y, Chan Y, Yang HY (2014) Dual wavelength electroluminescence from CdSe/CdS tetrapods. ACS Nano 8(3):2873–2879

    Article  CAS  PubMed  Google Scholar 

  • Wu X, Liu H, Liu J, Haley KN, Treadway JA, Larson JP, Ge N, Peale F, Bruchez MP (2003) Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots. Nat Biotechnol 21(1):41–46

    Article  CAS  PubMed  Google Scholar 

  • **ao Y, Barker PE (2004) Semiconductor nanocrystal probes for human metaphase chromosomes. Nucleic Acids Res 32(3):e28

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • **e H, Liu M, You B, Luo G, Chen Y, Liu B, Jiang Z, Chu PK, Shao J, Yu XF (2020) Biodegradable Bi2O2Se quantum dots for photoacoustic imaging-guided cancer photothermal therapy. Small 16(1):e1905208

    Article  PubMed  CAS  Google Scholar 

  • Yamamoto J, Oura M, Yamashita T, Miki S, ** T, Haraguchi T, Hiraoka Y, Terai H, Kinjo M (2015) Rotational diffusion measurements using polarization-dependent fluorescence correlation spectroscopy based on superconducting nanowire single-photon detector. Opt Express 23(25):32633–32642

    Article  PubMed  Google Scholar 

  • Yao J, Larson DR, Vishwasrao HD, Zipfel WR, Webb WW (2005) Blinking and nonradiant dark fraction of water-soluble quantum dots in aqueous solution. Proc Natl Acad Sci U S A 102(40):14284–14289

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yao J, Wang LV (2018) Recent progress in photoacoustic molecular imaging. Curr Opin Chem Biol 45:104–112

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yu WW, Chang E, Falkner JC, Zhang J, Al-Somali AM, Sayes CM, Johns J, Drezek R, Colvin VL (2007) Forming biocompatible and nonaggregated nanocrystals in water using amphiphilic polymers. J Am Chem Soc 129(10):2871–2879

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Yang Q, Cao H, Ratcliffe CI, Kingston D, Chen QY, Ouyang JY, Wu XH, Leek DM, Riehle FS, Yu K (2016) Bright gradient-alloyed CdSexS1−x quantum dots exhibiting cyan-blue emission. Chem Mater 28:618–625

    Article  CAS  Google Scholar 

  • Zhao P, Xu Q, Tao J, ** Z, Pan Y, Yu C, Yu Z (2018) Near infrared quantum dots in biomedical applications: current status and future perspective. Wiley Interdiscip Rev Nanomed Nanobiotechnol 10(3):e1483

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Kobe, Japan

    Fumihiko Fujii

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  1. Fumihiko Fujii
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Correspondence to Fumihiko Fujii .

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Editors and Affiliations

  1. Department of Convergence Medicine, University of Ulsan College of Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea (Republic of)

    Jun Ki Kim

  2. Department of Radiology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea (Republic of)

    Jeong Kon Kim

  3. Department of Convergence Medicine, University of Ulsan College of Medicine, Asan Institute for Life Sciences, Asan Medical Center, Seoul, Korea (Republic of)

    Chan-Gi Pack

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Fujii, F. (2021). Semiconductor Nanocrystals for Biological Imaging and Fluorescence Spectroscopy. In: Kim, J.K., Kim, J.K., Pack, CG. (eds) Advanced Imaging and Bio Techniques for Convergence Science. Advances in Experimental Medicine and Biology, vol 1310. Springer, Singapore. https://doi.org/10.1007/978-981-33-6064-8_16

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