Abstract
Currently, most wound dressings are therapeutically inert, lacking active components to aid in the wound healing process. In addition, production is based on mass subtractive manufacturing methods and does not allow personalised application. To address these issues, we explored the formulation of a composite gellan gum (GG)-hydroxypropyl methylcellulose (HPMC) hydrogel via additive manufacturing capable as a delivery vehicle for antimicrobials. Extrusion-based 3D printing was chosen as the mode of hydrogel production. Rheological tests were conducted to optimise the hydrogel formulation starting from a GG: HPMC ratio of 1:1 derived from previous studies. The optimal formulation, found to be 1 GG: 2 HPMC, was most able to recover from stress and display consistent time-dependent mechanical behaviour. The final 3D-printing conditions comprised of pneumatic pressure of 180 kPa, printhead speed of 5 mm/s and 25% infill density resulted in a 2 cm × 2 cm × 0.8 cm film. Subsequently, this formulation was tested as a delivery system for colistin, an approved peptide antimicrobial via agar well diffusion studies using Escherichia coli. Our results showed that our hydrogel formulation is an effective drug delivery vehicle and can be reproducibly printed to custom specifications. Taken together, this study demonstrates the feasibility of a customisable, “on-demand” therapeutic active hydrogel wound dressing for use in clinical settings.
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Pan, J., Lee, J., Cheong, R. (2023). Development of a 3D-Printable Hydrogel-Based Antimicrobial Film. In: Guo, H., et al. IRC-SET 2022. Springer, Singapore. https://doi.org/10.1007/978-981-19-7222-5_27
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DOI: https://doi.org/10.1007/978-981-19-7222-5_27
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