Abstract
In this chapter, I will discuss the technology that increases the quality of the highly complex oligo libraries that is one of the most essential points for the efficient synthetic nucleic acids storage.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Yeom H et al (2020) Cell-free bacteriophage genome synthesis using low-cost sequence-verified array-synthesized oligonucleotides. ACS Synth Biol 9(6):1376–1384. https://doi.org/10.1021/acssynbio.0c00051
Tian J et al (2004) Accurate multiplex gene synthesis from programmable DNA microchips. Nature 432(7020):1050–1054. https://doi.org/10.1038/nature03151
Ong LL et al (2017) Programmable self-assembly of three-dimensional nanostructures from 10,000 unique components. Nature 552(7683):72–77. https://doi.org/10.1038/nature24648
Organick L et al (2018) Random access in large-scale DNA data storage. Nat Biotechnol 36(3):242–248. https://doi.org/10.1038/nbt.4079
Zhou Y et al (2014) High-throughput screening of a CRISPR/Cas9 library for functional genomics in human cells. Nature 509(7501):487–491. https://doi.org/10.1038/nature13166
Bai X, Kim J, Kang S, Kim W, Shim H (2015) A novel human scFv library with non-combinatorial synthetic CDR diversity. PLoS ONE 10(10):1–18. https://doi.org/10.1371/journal.pone.0141045
Ma S, Saaem I, Tian J (2012) Error correction in gene synthesis technology. Trends Biotechnol 30(3):147–154. https://doi.org/10.1016/j.tibtech.2011.10.002
Kosuri S, Church GM (2014) Large-scale de novo DNA synthesis: technologies and applications. Nat Methods 11(5):499–507. https://doi.org/10.1038/nmeth.2918
Ellington A, Pollard JD (2009) Introduction to the synthesis and purification of oligonucleotides. Curr Protoc Nucleic Acid Chem 38:1–22. https://doi.org/10.1002/0471142700.nca03cs00
Pinto A, Chen SX, Zhang DY (2018) Simultaneous and stoichiometric purification of hundreds of oligonucleotides. Nat Commun 9(1):1–9. https://doi.org/10.1038/s41467-018-04870-w
Wan W et al (2014) Error removal in microchip-synthesized DNA using immobilized MutS. Nucleic Acids Res 42(12):1–14. https://doi.org/10.1093/nar/gku405
Fuhrmann M, Oertel W, Berthold P, Hegemann P (2005) Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage. Nucleic Acids Res 33(6):1–8. https://doi.org/10.1093/nar/gni058
Carr PA, Park JS, Lee YJ, Yu T, Zhang S, Jacobson JM (2004) Protein-mediated error correction for de novo DNA synthesis. Nucleic Acids Res 32(20):1–9. https://doi.org/10.1093/nar/gnh160
Till BJ, Burtner C, Comai L, Henikoff S (2004) Mismatch cleavage by single-strand specific nucleases. Nucleic Acids Res 32(8):2632–2641. https://doi.org/10.1093/nar/gkh599
Lee H et al (2015) A high-throughput optomechanical retrieval method for sequence-verified clonal DNA from the NGS platform. Nat Commun 6. https://doi.org/10.1038/ncomms7073
Matzas M et al (2010) High-fidelity gene synthesis by retrieval of sequence-verified DNA identified using high-throughput pyrosequencing. Nat Biotechnol 28(12):1291–1294. https://doi.org/10.1038/nbt.1710
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
Copyright information
© 2023 The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.
About this chapter
Cite this chapter
Choi, H. (2023). Synthetic Nucleic Acids Storage. In: Purifying and Indexing Technology for Nucleic Acids-Based Next Generation Storage Medium. Springer Theses. Springer, Singapore. https://doi.org/10.1007/978-981-19-4274-7_2
Download citation
DOI: https://doi.org/10.1007/978-981-19-4274-7_2
Published:
Publisher Name: Springer, Singapore
Print ISBN: 978-981-19-4273-0
Online ISBN: 978-981-19-4274-7
eBook Packages: EngineeringEngineering (R0)