Abstract
Donepezil hydrochloride, an anticholinesterase drug, is orally administered to patients suffering from Alzheimer’s disease. Alzheimer’s is a neurodegenerative disorder characterized by the degradation of neurotransmitters, neuronal apoptosis, and loss of synapses. The treatment of such neural diseases is hampered by the presence of a tightly controlled blood–brain barrier (BBB), which prevents the influx of drugs. The intranasal route of drug delivery has been lately seen as a promising approach to deliver drugs directly to the brain, effectively bypassing the BBB, as it offers high absorption and increased bioavailability. In this study, we have fabricated hybrid polymeric lipid nanoparticles with two natural polymers—chitosan and gelatin. The average particle sizes of chitosan lecithin and gelatin lecithin nanoparticles were 237.43 and 278.86 nm. The percentage drug loading for chitosan lecithin and gelatin lecithin nanoparticles was 10.24 ± 0.4 and 8.77 ± 0.748%. The chitosan lecithin nanoparticles showed a burst release of up to 99.99% drug for 5 days, while the gelatin lecithin nanoparticles exhibited a sustained release of 33.31% drug for 30 days under acidic conditions. The cell viability studies showed that both nanoparticles were safe toward mouse fibroblast cells (L929). Finally, both nanoparticles were found to be mucoadhesive in nature. Out of the two nanoparticulate systems developed, the gelatin lecithin nanoparticles demonstrates a strong potential as a carrier for donepezil delivery.
Meghana Bhandari and Nahida Rasool-both authors contributed equally to the work
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Acknowledgements
Financial assistance from the DBT India to Y.S. (BT/PR40669/MED/32/761/2020) is gratefully acknowledged. We also thank the Departments of Biomedical Engineering and Chemistry for providing the access to their research facilities. M.B. and N.R. received their fellowships from IIT Ropar.
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Bhandari, M., Rasool, N., Singh, Y. (2022). Polymeric Lipid Nanoparticles for Donepezil Delivery. In: Gupta, B., Jawaid, M., Kaith, B.S., Rattan, S., Kalia, S. (eds) Polymeric Biomaterials and Bioengineering. Lecture Notes in Bioengineering. Springer, Singapore. https://doi.org/10.1007/978-981-19-1084-5_5
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