Abstract
In this chapter, a method for the determination of milk urea nitrogen (MUN) using short wavelength near-infrared (NIR) analysis of non-homogenized raw milk will be presented. NIR spectra of 183 non-homogenized milk samples from seven early lactation cows with different health statuses (healthy and diagnosed with mastitis) were measured at 40 °C, in the NIR region 700–1,100 nm. All cows were housed in the same conditions and fed the same diet. Partial least squares regression (PLSR) analysis was used for quantification of MUN in raw milk, based on the acquired NIR spectra with leave-one-animal-out cross-validation. Animals which contracted mastitis during the trials were identified, and the results for MUN prediction were separated from the results for healthy animals. The results of PLSR analysis showed excellent correlation between the values of MUN estimated based on the developed model and reference MUN values measured by the enzymatic method (R = 0.906). A standard error of 1.09 mg/dl, which is suitable for real-time MUN monitoring of raw milk, was achieved in the analysis of the milk of healthy animals. The NIRS method may provide a valuable tool to monitor urea nitrogen in routine measurements of milk for feeding management in dairy.
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Tsenkova, R., Muncan, J. (2022). Milk Urea Nitrogen Measurement. In: Aquaphotomics for Bio-diagnostics in Dairy. Springer, Singapore. https://doi.org/10.1007/978-981-16-7114-2_7
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