Abstract
Marine habitats are still largely unexplored for their microbial diversity and ecosystem analysis. Among the marine microbial population, the actinomycetes are significant component. The actinomycetes are gram-positive bacteria with high G+C contents, displaying filamentous cell morphology and chalky white, powdery texture of the colonies. Isolation and cultivation of the actinomycetes is a difficult and tricky part of their study. Cultivation approaches can be bifurcated into the traditional and high-throughput techniques. Designing suitable media mimicking the in situ native conditions is highly significant to trap the majority of the actinomycetes diversity of the marine habitat. Molecular techniques like ARDRA, DGGE, TGGE and habitat or group specific signature approaches are used to investigate the taxonomy and diversity of the actinomycetes. Nowadays, newer approaches, such as metagenomics and metabolomics, are explored to investigate and analyze community-level diversity and metabolic behavior of the marine actinomycetes. These microorganisms play vital role in the ecosystem and project potential avenues for various applications.
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Acknowledgments
The work cited in this review from the SPS laboratory at the Saurashtra University was supported under the UGC-CAS Program, DST-FIST, DBT-Multi-Intuitional Project, MoES (Government of India) Net Working Project, and the Saurashtra University. SPS acknowledges DST-SERB International Travel Fellowships to present his work in Hamburg (Germany), Cape Town (South Africa), and Kyoto (Japan). AKS is grateful for the UGC Basic Science Research (BSR) Fellowship and International Travel Grants from DST-SERB, DBT, and Centre for International Co-operation in Science (CICS) to present his work in Canada. A.D. acknowledges the award of SHoDH Fellowship by the Gujarat Government. SPS also acknowledges award of UGC BSR Faculty Fellowship. DR acknowledges JRF/SRF under the MoES-Net Working Project, New Delhi.
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Rathore, D.S., Sharma, A.K., Dobariya, A., Ramavat, H., Singh, S.P. (2022). Cultivation and Diversity of Marine Actinomycetes: Molecular Approaches and Bioinformatics Tools. In: Karthik, L. (eds) Actinobacteria. Springer, Singapore. https://doi.org/10.1007/978-981-16-5835-8_12
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