Abstract
Because of the important role of preclinical animal studies in the development of innovative medicines for human patients, many stem cell therapies have been evaluated in animals. However, the last decade has seen the beginning of a shift from stem cell treatments in animals only for the benefit of human patients to including new therapeutic development of tissue stem cells primarily for animal care. Not surprisingly, given their historical dependency, the new field of veterinary stem cell medicine faces many of the same challenges as human stem cell medicine. In this chapter, a shared major deficiency, the lack of stem cell-specific dosing, is considered from the perspective that implementing dosing would accelerate progress in veterinary stem cell medicine and human stem cell medicine as well, as a follow-on. Since the vast majority of present-day veterinary stem cell treatments utilize preparations of mesenchymal stem cells (MSCs), the well-recognized uncertainties about this treatment source are discussed. The challenges of quantifying the stem cell-specific dose of MSC preparations exemplify the general problem of determining the stem cell dose of all stem cell treatments. Particular consideration is given to previous veterinary MSC treatment studies that include measures that might relate to stem cell dosage. Kinetic stem cell counting, a first potential solution to the tissue stem cell dosing problem, is described, and the potential benefits of its future use are discussed. Adoption of kinetic stem cell counting into the general practice of veterinary stem cell medicine is presented as the key that can unlock the full potential of stem cells in veterinary medical practice and perhaps human stem cell medical practice as well.
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Research reported in this publication was supported by the National Heart, Lung, and Blood Institute of the National Institutes of Health under Award Number R43HL154900. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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Boutin, S.R., Sherley, J.L. (2021). Advancing Quantitative Stem Cell Dosing for Veterinary Stem Cell Medicine. In: Choudhary, R.K., Choudhary, S. (eds) Stem Cells in Veterinary Science. Springer, Singapore. https://doi.org/10.1007/978-981-16-3464-2_12
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