Abstract
To elucidate neural mechanisms underlying oscillatory phenomena in brain function, we have developed optogenetic tools and statistical methods. Specifically, opto-current-clamp induced oscillation reveals intrinsic frequency preferences in the neural circuits by oscillatory resonance. Furthermore, resonance or entrainment to intrinsic frequency is state-dependent. When resonance phenomena go beyond a certain range, it could even induce epileptic seizure in highly reproducible manner. We are able to study how seizures start, develop, and stop in neural circuits. Therefore, the optogenetics-induced oscillatory activation is a powerful tool in neuroscience research
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Abbreviations
- GAD:
-
Glutamic acid decarboxylase
- MI:
-
Caudal motor area
- MII:
-
Rostral motor area
- PV:
-
Parvalbumin
- RSSL:
-
Rectified sinusoidal sweep of light
- SOM:
-
Somatostatin
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Acknowledgments
This work was supported by JSPS KAKENHI Grant Number JP16H06276 (Platform of Advanced Animal Model Support) and MEXT KAKENHI Grant Number 15H05879 (Non-linear Neuro-oscillology) and this research was supported by AMED under Grant Number JP19dm0207001.
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Mushiake, H. et al. (2021). Multimodal Functional Analysis Platform: 4. Optogenetics-Induced Oscillatory Activation to Explore Neural Circuits. In: Yawo, H., Kandori, H., Koizumi, A., Kageyama, R. (eds) Optogenetics. Advances in Experimental Medicine and Biology, vol 1293. Springer, Singapore. https://doi.org/10.1007/978-981-15-8763-4_34
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DOI: https://doi.org/10.1007/978-981-15-8763-4_34
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