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Cyanobacterial Enzymes for Bioalkane Production

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Synthetic Biology of Cyanobacteria

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1080))

Abstract

Cyanobacterial biosynthesis of alkanes is an attractive way of producing substitutes for petroleum-based fuels. Key enzymes for bioalkane production in cyanobacteria are acyl-ACP reductase (AAR) and aldehyde-deformylating oxygenase (ADO). AAR catalyzes the reduction of the fatty acyl-ACP/CoA substrates to fatty aldehydes, which are then converted into alkanes/alkenes by ADO. These enzymes have been widely used for biofuel production by metabolic engineering of cyanobacteria and other organisms. However, both proteins, particularly ADO, have low enzymatic activities, and their catalytic activities are desired to be improved for use in biofuel production. Recently, progress has been made in the basic sciences and in the application of AAR and ADO in alkane production. This chapter provides an overview of recent advances in the study of the structure and function of AAR and ADO, protein engineering of these enzymes for improving activity and modifying substrate specificities, and examples of metabolic engineering of cyanobacteria and other organisms using AAR and ADO for biofuel production.

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Acknowledgments

This work was supported by Grants-in-Aids for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) and by the Institute for Fermentation, Osaka.

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Authors and Affiliations

  1. Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan

    Munehito Arai, Yuuki Hayashi & Hisashi Kudo

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  1. Munehito Arai
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  2. Yuuki Hayashi
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  3. Hisashi Kudo
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Correspondence to Munehito Arai .

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Editors and Affiliations

  1. School of Chemical Engineering, Tian** University, Tian**, China

    Weiwen Zhang

  2. Center for Biosafety Research and Strategy, Tian** University, Tian**, China

    **nyu Song

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Arai, M., Hayashi, Y., Kudo, H. (2018). Cyanobacterial Enzymes for Bioalkane Production. In: Zhang, W., Song, X. (eds) Synthetic Biology of Cyanobacteria. Advances in Experimental Medicine and Biology, vol 1080. Springer, Singapore. https://doi.org/10.1007/978-981-13-0854-3_6

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