Abstract
Plasma fibronectin (FN) is synthesized by hepatocytes and secreted into the circulation in a soluble, compact and non-fibrillar form. However, intrinsic functions of FN in the body are prevalent to the multimeric FN fibrils. Plasma FN is assembled by cells or adherent platelets into functional fibrils. Reports have indicated that the process to incorporate FN into multimer fibrils can also occur in cell-free models in vitro by incubation with denaturants, reducing agents, or anastellin (FN peptidic fragment). Here, we report on (1) the formation of insoluble fibrillar-like supramolecules of plasma FN (FN fibrils) by exposing the molecules to increasing shear rates and (2) the functional characterization in platelet adhesion and aggregation.
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Acknowledgements
This work was supported by the NRW Research School BioStruct, by grants from the Ministry of Innovation, Science, Research and Technology of the German Federal State North Rhine-Westphalia (NRW) and from the ‘Gründerstiftung zur Förderung von Forschung und wissenschaftlichen Nachwuchs an der Heinrich-Heine-Universität Düsseldorf’. Moreover, some equipments of this work have been supported by grant No. 1161/QĐ-ĐHQG-KHCN of Vietnam National Universities-HCM City.
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Khon, H. et al. (2018). Shear-Induced Fibrillar-Like Supramolecule of Plasma Fibronectin: A New Form of Fibronectin with Enhanced Activity in Platelet Adhesion and Aggregation. In: Vo Van, T., Nguyen Le, T., Nguyen Duc, T. (eds) 6th International Conference on the Development of Biomedical Engineering in Vietnam (BME6) . BME 2017. IFMBE Proceedings, vol 63. Springer, Singapore. https://doi.org/10.1007/978-981-10-4361-1_137
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DOI: https://doi.org/10.1007/978-981-10-4361-1_137
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