Abstract
An in-house developed Label-free Immobilization-free Terahertz (THz) sensor has shown its potential in various applications [1]. In this paper, we present the experimental results in the field of High Performance Liquid Chromatography (HPLC) and protein crystallization. Ultra-Violet (UV) detectors are very commonly used as a detection technique in HPLC systems. However, it relies on labelling when target substances do not absorb UV light. Therefore, it has the drawback of increased cost and time consumption. Our experimental result shows that THz sensor has the potential to replace UV sensor integrated on the HPLC machine as it is able to detect both UV and non-UV absorbing substances without labelling. Meanwhile, the THz sensor is also deployed for the first time to follow a protein crystallization process.
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Acknowledgments
The authors acknowledge the Vrije Universiteit Brussel (VUB) through the SRP-project M3D2, the FWO-Vlaanderen through FWOAL682 “Building blocks of lab-on-chip system for label-free monitoring of bio-molecular interactions” and the FWOAL611 “Millimeter wave sensor solutions for chromatographic analyzer systems of today and tomorrow”, the, the COST-action MP1204, TERAMIR the NATO support for the Workshop on THz Diagnostics of CBRN effects and Detection of Explosives & CBRN.
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Zhang, Y. et al. (2017). Bio-chemical Process Monitoring with Terahertz Sensor. In: Pereira, M., Shulika, O. (eds) THz for CBRN and Explosives Detection and Diagnosis. NATO Science for Peace and Security Series B: Physics and Biophysics. Springer, Dordrecht. https://doi.org/10.1007/978-94-024-1093-8_4
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DOI: https://doi.org/10.1007/978-94-024-1093-8_4
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