Abstract
This paper describes the reduction of the size of enzyme immunoassay (EIA) utilizing microchemical reaction in a microchamber array. The microchamber array was fabricated by micromachining of a silicon chip. Glass beads of 100 µm were immobilized with antibody, and put in the microchambers of 150 p.m. As a competitive ELISA, sample solution mixed with HRP-conjugated antigens was allowed to react in the microchamber. As a sandwich assay, sample solution and HRP-conjugated antibody were sequentially added to the chamber. The addition of buffer, hydrogen peroxide, and fluorogenic substrate produced fluorescent dye, and a fluorescence microscope observed its fluorescence.
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© 2000 Springer Science+Business Media Dordrecht
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Murakami, Y., Kikuchi, T., Yanase, M., Nagai, H., Morita, Y., Tamiya, E. (2000). Microchamber Array for Immunosensor Applications. In: van den Berg, A., Olthuis, W., Bergveld, P. (eds) Micro Total Analysis Systems 2000. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2264-3_45
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DOI: https://doi.org/10.1007/978-94-017-2264-3_45
Publisher Name: Springer, Dordrecht
Print ISBN: 978-90-481-5496-8
Online ISBN: 978-94-017-2264-3
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