Abstract
Transposon Tn5 mutagenesis in Pseudomonas fluorescens strain CN12 expressed increased in vitro antibiosis against Gaeumannomyces graminis var. tritici and provided enhanced biological control of the take-all disease of wheat. In bacterial conjugation experiments, Tn5-induced mutants to both increased and decreased or loss of antibiosis were obtained. The rate of mutation to increased antibiosis and loss of antibiosis was 0.1% and 0.4%, respectively. In greenhouse pot tests, mutants with increased antibiosis were significantly more suppressive to take-all than wild type strain CN12. Antibiosis-negative mutants lost their abilities to suppress take-all. In a typical field experiment to control take-all, seed treatment with D93, the best Tn5 mutant increased wheat grain yield by 27.2% over no bacteria control, whileas its parent stain CN12 increased yield only by 12.3%. In field experiments conducted in spring and winter wheat during 1988-1991, treatment with D93 reduced whiteheads 65% and increased yield 25 % on average, compared to no bacteria treatment.
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© 1993 Springer Science+Business Media Dordrecht
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Peng, Y., Zhang, Z., Huang, D., Chen, C., Zhang, J. (1993). Enhanced Biological Control of Wheat Take-All By Using In Vivo Genetically Engineered Pseudomonas Fluorescens . In: You, C., Chen, Z., Ding, Y. (eds) Biotechnology in Agriculture. Current Plant Science and Biotechnology in Agriculture, vol 15. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-1779-1_88
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DOI: https://doi.org/10.1007/978-94-011-1779-1_88
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