Abstract
Many medically important insects, particularly in the order Diptera, have tissues with readily accessible, giant polytene chromosomes that can be used to physically map fragments of DNA or RNA. The technique of polytene chromosome in situ hybridization was developed and first used with Drosophila chromosomes (Gall and Pardue, 1969; Atherton and Gall, 1972), but it has since been used effectively with a variety of other Diptera, including chironomids, sciarids, several cydorraphan flies, and anopheline mosquitoes (e.g., Bedo and Howells, 1987; Schmidt et al., 1988; Graziosi et al., 1990; Zacharopoulou et al., 1992; Kumar and Collins, 1994). DNA probes have been mapped by in situ hybridization to the polytene chromosomes of a number of Anopheles species using fluorescence in situ hybridization (FISH) (Marchi and Pili, 1994) and by using biotin-labelled DNA probes (e.g. Graziosi et al., 1990; Kumar and Collins, 1994; Salazar et al., 1994; Besansky et al., 1994).
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Kumar, V., Cornel, A.J., Mukabayire, O. (1997). In situ hybridization to Anopheles polytene chromosomes . In: Crampton, J.M., Beard, C.B., Louis, C. (eds) The Molecular Biology of Insect Disease Vectors. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-1535-0_29
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DOI: https://doi.org/10.1007/978-94-009-1535-0_29
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