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The Detection of High Mass-to-Charge Biological Ions by Fourier Transform Mass Spectrometry: Issues and Routes for Instrument Improvement

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Mass Spectrometry in Biomolecular Sciences

Part of the book series: NATO ASI Series ((ASIC,volume 475))

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Abstract

Although Fourier transform mass spectrometry (FTMS) has demonstrated the ability to trap biomolecular ions that have mass-to-charge ratios on the order of 150000, FTMS is seldom employed as a tool for the characterization of biological molecules. This is because the superior detection performance that is associated with FTMS rapidly degrades for ions with mass-to-charge ratios greater than 5000. The purpose of this chapter is to introduce the reader to the issues that stand in the way of the development of routine high mass-to-charge FTMS and to the recent advances and strategies that are extending the impressive capabilities of FTMS to singly-charged, biological molecules.

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Authors and Affiliations

  1. Midwest Center for Mass Spectrometry, Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, 68588-0362, USA

    Christopher L. Holliman, Don L. Rempel & Michael L. Gross

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  1. Christopher L. Holliman
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  2. Don L. Rempel
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  3. Michael L. Gross
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Editors and Affiliations

  1. Medical School, University of Texas, Houston, Texas, USA

    Richard M. Caprioli

  2. SESMA-C.N.R, Napoli, Italy

    Antonio Malorni

  3. Dipartimento di Chimica, Università della Calabria, Arcavacata di Rende, Italy

    Giovanni Sindona

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© 1996 Kluwer Academic Publishers

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Holliman, C.L., Rempel, D.L., Gross, M.L. (1996). The Detection of High Mass-to-Charge Biological Ions by Fourier Transform Mass Spectrometry: Issues and Routes for Instrument Improvement. In: Caprioli, R.M., Malorni, A., Sindona, G. (eds) Mass Spectrometry in Biomolecular Sciences. NATO ASI Series, vol 475. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-0217-6_8

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  • DOI: https://doi.org/10.1007/978-94-009-0217-6_8

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