Summary
Spin-lattice relaxation times (T1) of water protons and cross-relaxation times (TIS) between irradiated protein protons and observed water protons were measured to study water structure in various rat brain tissue. A tumour-bearing rat model and an incomplete forebrain ischaemia model of stroke prone spontaneously hypertensive rats (SHRSP) were used for the experiments. TIS and T1 ere measured by the inversion recovery FT method with and without f2-irradiation at 7.13 p.p.m. and γ H2/2π ~ 69 Hz, respectively, using a 360 MHz FT-NMR spectrometer. All of experiments on T1 and TIS yielded a single kind of component. In spite of small changes in T1 and water content, TIS values in peritumoural oedematous and ischaemic brain tissue were significantly shorter than those of normal brain and far shorter than those of brain tumour, indicating a large amount of hydration of marco-molecules in the oedematous and/or ischaemic rat brain tissue. TIS might be sensitive parameter for studying the water structure in various brain tissue.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
I. Akasaka K (1981) Longitudinal relaxation of protons under cross saturation and spin diffusion. J Magn Reson 45: 337–343
Akasaka K (1983) Spin diffusion and the dynamic structure of a protein. Streptomyces subtilisin inhibitor. J Magn Reson 51: 14–25
Bakay L, Kurland RJ, Parrish RG, Lee JC, Peng RJ, Bartkowski HM (1975) Nuclear magnetic resonance studies in normal and oedematous brain tissue. Exp Brain Res 23: 241–248
Beall PT, Hazlewood CF, Rao PN (1976) Nuclear magnetic resonance patterns of intracellular water as a function of HeLa cell cycle. Science 192: 904–907
Bederson JB, Bartkowski HM, Moon K, Halks-Miller M, Nishimura MC, Brant-Zawadski M, Pitts LH (1986) Nuclear magneitc resonance imaging and spectroscopy in experimental brain oedema in a rat model. J Neurosurg 64: 795–802
Era S, Kato K, Sogami M, Nagaoka S, Kuwata K, Takahashi M, Suzuki E, Miura K, Watari H, Akasaka K (1986) Comparative 1 H NMR studies on water structure in hepatic tissues and protein gel. Biomed Res 7 [Suppl 2]: 41–46
Era S, Sogami M, Kuwata K, Fujii H, Suzuki E, Miura K, Kato K, Watari H (1989) Hnmr. studies on cross-relaxation phenomena in bovine mercaptalbumin (BMA) solution and partially hydrolyzed bovine plasma albumin (BPA*) gel. Int J Peptide Protein Res 33: 214–222
Go KG, Edzes HT (1975) Water in brain oedema. Observations by the pulsed nuclear magnetic resonance technique. Arch Neurol 32: 462–465
Horikawa Y, Naruse S, Tanaka C, Hirakawa K, Nishikawa H (1986) Proton NMR relaxation times in ischaemic brain oedema. Stroke 17: 1149–1152
Kuntz ID, Zipp A (1977) Water in biological systems. N Eng J Med 297: 262–266
Naruse S, Horikawa Y, Tanaka C, Hirakawa K, Nishikawa H, Yoshizaki K (1982) Proton nuclear magnetic resonance studies on brain oedema. J Neurosurg 56: 747–752
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer-Verlag
About this paper
Cite this paper
Iwama, T. et al. (1990). 1H-NMR Studies on Water Structures in the Rat Brain Tissues with Cerebral Tumour or Ischaemia. In: Reulen, HJ., Baethmann, A., Fenstermacher, J., Marmarou, A., Spatz, M. (eds) Brain Edema VIII. Acta Neurochirurgica, vol 51. Springer, Vienna. https://doi.org/10.1007/978-3-7091-9115-6_42
Download citation
DOI: https://doi.org/10.1007/978-3-7091-9115-6_42
Publisher Name: Springer, Vienna
Print ISBN: 978-3-7091-9117-0
Online ISBN: 978-3-7091-9115-6
eBook Packages: Springer Book Archive