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Proteinarrays

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Immunoassays

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Zusammenfassung

Seit Ende der 1980er-Jahre und spätestens nach der Fertigstellung der Sequenzierung des menschlichen Genoms im Jahr 2003 stieg das Interesse an der Entwicklung und Anwendung von Microarrays zur Analyse von Genexpressionsmustern und Einzelnucleotid-Polymorphismen stetig. Die Microarray-Technologie hat sich seitdem laufend weiterentwickelt und ist aus der Werkzeugkiste der Lebenswissenschaften nicht mehr wegzudenken. Proteinarrays spielen ihre Stärken durch einen geringen Probenverbrauch und einen geringen Verbrauch an Reagenzien, kombiniert mit einer hohen Probenkapazität, im Vergleich zu klassischen gelbasierten Proteomik-Techniken aus. Mit der Ambient Analyte Theory hat Roger Ekins die theoretischen und praktischen Grundlagen für Multi-Spot und Multi-Analyt-Immunoassays gelegt (Ekins 1990). Im Vergleich zu DNA-Microarrays stellen Proteinarrays aber auch einige Herausforderungen.

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Authors and Affiliations

  1. Boehringer Ingelheim VRC GmbH & Co. KG, Hannover, Deutschland

    Johanna Sonntag & Matthias Griessner

Authors
  1. Johanna Sonntag
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  2. Matthias Griessner
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Correspondence to Matthias Griessner .

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Editors and Affiliations

  1. Centrum für Nanotechnologie, Universität Münster, Münster, Nordrhein-Westfalen, Deutschland

    Arnold Maria Raem

  2. CANDOR Bioscience GmbH, Wangen im Allgau, Deutschland

    Peter Rauch

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Sonntag, J., Griessner, M. (2023). Proteinarrays. In: Raem, A.M., Rauch, P. (eds) Immunoassays. Springer Spektrum, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-62671-9_10

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