Abstract
Polymerase chain reaction (PCR) was performed to generate restriction site-flanked dsDNA for cloning approaches as well as dsDNA for verifying genomic integration of myc-Survivin into Flp-InTM T-RexTM HeLa cells. Therefore, the Expand High Fidelity PCR System (Roche) was used.
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© 2017 Springer Fachmedien Wiesbaden GmbH
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Dannheisig, D. (2017). Methods. In: Impact of Survivin Acetylation on its Biological Function. BestMasters. Springer Spektrum, Wiesbaden. https://doi.org/10.1007/978-3-658-18623-4_3
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DOI: https://doi.org/10.1007/978-3-658-18623-4_3
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Publisher Name: Springer Spektrum, Wiesbaden
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Online ISBN: 978-3-658-18623-4
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