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Two-Dimensional 31P-1H Correlation Spectroscopy in Intact Organs and Their Extracts

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In-Vivo Magnetic Resonance Spectroscopy II: Localization and Spectral Editing

Part of the book series: NMR Basic Principles and Progress ((NMR,volume 27))

Abstract

2D 31P-1H correlation spectroscopy is a useful technique for the assignment of 31P-NMR resonances. It allows the separation of overlap** 31P peaks by spreading them in the 1H dimension and gives the chemical shifts of the protons which are spin-spin coupled to the phosphorus atoms. In this chapter we give a short survey of the various 2D heteronuclear techniques with an emphasis on those that might be useful for 31P-1H correlation in extracts, intact tissues, and in vivo. Examples are given for such applications in intact pancreases excised from healthy rats and from rats with experimental pancreatitis, in excised pancreatic carcinoma, as well as for extracts from rat pancreas and liver. The potential of 2D 31P-1H correlation spectroscopy for the study of isolated and in-vivo systems is discussed.

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Abbreviations

ATP:

adenosine triphosphate

COSY:

2D homonuclear correlation spectroscopy

DPDE:

diphosphodiesters

GPC:

glycerophosphocholine

GPE:

glycerophosphoethanolamine

HETCOSY:

2D heteronuclear correlation spectroscopy

HOHAHA:

homonuclear Hartmann Hahn cross polarization

PCh:

phosphocholine

PCr:

phosphocreatine

PDE:

phosphodiesters

PEA:

phosphoethanolamine

Pi:

inorganic phosphate

PME:

phosphomonoesters

Pur 3′:

purine 3′-phosphate

Pur 2′:

purine 2′-phosphate

Pyr 3′:

pyrimidine 3′-phosphate

Pyr 2′:

pyrimidine 2′-phosphate

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Author information

Authors and Affiliations

  1. School of Chemistry, Sackler Faculty of Exact Sciences, Tel Aviv University, Tel Aviv, 69978, Israel

    Gil Navon & Nadir Askenasy

  2. Department of Diagnostic Imaging MRI Unit, Sheba Medical Center, Tel Hashomer, 52621, Israel

    Tammar Kushnir

  3. Department of Surgery, Tel Aviv Medical Center, Rokach Hospital, Tel Aviv, Israel

    Ofer Kaplan

Authors
  1. Gil Navon
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  2. Tammar Kushnir
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  3. Nadir Askenasy
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  4. Ofer Kaplan
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Editor information

Editors and Affiliations

  1. Preclinical Research 386/206, Sandoz Pharma Ltd., Ch-4002, Basel, Switzerland

    M. Rudin

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© 1992 Springer-Verlag, Berlin Heidelberg

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Navon, G., Kushnir, T., Askenasy, N., Kaplan, O. (1992). Two-Dimensional 31P-1H Correlation Spectroscopy in Intact Organs and Their Extracts. In: Rudin, M. (eds) In-Vivo Magnetic Resonance Spectroscopy II: Localization and Spectral Editing. NMR Basic Principles and Progress, vol 27. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-77208-5_10

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  • DOI: https://doi.org/10.1007/978-3-642-77208-5_10

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  • Print ISBN: 978-3-642-77210-8

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