Abstract
Bovine aortic endothelium can form, in vitro, a confluent monolayer of small and strictly non-overlap** cells which retain the differentiated properties of their in vivo counterparts (Vlodavsky et al. 1979). The localization and the dynamic properties of the lipophilic probe 5N-(hexadecanoyl)-aminofluorescein (HEDAF) was observed to be markedly influenced by the presence of cell junctions. The probe was found to be restricted to the apical plasmalemma by junctional complexes. The probe was only observed in the basal membrane after disruption of cell conctacts. Fluorescence Recovery After Photobleaching (FRAP) experiments indicated that the lateral diffusion coefficient D of the probe on apical membranes fell when the cell junctions were disrupted, but returned to its initial value when cell contacts were restored.
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© 1988 Springer-Verlag Berlin Heidelberg
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Tournier, J.F., Lopez, A., Gas, N., Tocanne, J.F. (1988). Lipid Localization and Mobility in the Plasmalemma of Aortic Endothelial Cells are Reversibly Affected by the Presence of Cell Junctions. In: Op den Kamp, J.A.F. (eds) Membrane Biogenesis. NATO ASI Series, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73184-6_3
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DOI: https://doi.org/10.1007/978-3-642-73184-6_3
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