Effects of PSD007-Based Photodynamic Therapy in Murine Hepatoma MM45T-Li Cells

Abstract

the aim of this study was to investigate the effects of PSD007-based photodynamic therapy on MM45T-Li cells. The uptake concentration of PSD007 was observed by fluorescent microscopy. Fluorescent intensity of different concentrations PSD007 with various incubation time was assayed by multi microplates reader. The photodynamic therapy treatment on cells was performed in vitro using PSD007 as photosensitizer and 630 nm laser as light source. The proliferation activity of cells was evaluated by MTT assay after photodynamic therapy. The results showed that 20μg/ml, 40μg/ml, 80μg/ml and 160 μg/ml of PSD007 were absorbed in cytoplasm after incubating with cells for 3 h. The fluorescent intensity of PSD007 increased with extending incubating time whereas the uptake tended to saturation when incubating time extended to 4 h. 40μg/ml of PSD007 which incubated with cells for 3 h was selected in photodynamic therapy treatment. The survival rate of cells decreased with the increase of energy density from 0.9 to 7.2 J/cm². The survival rate dropped to 5.5% when energy density was raised to 7.2 J/cm². In conclusion, PSD007-based photodynamic therapy could efficiently inhibited the proliferation of murine hepatoma MM45T-Li cells.