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Methods to Isolate and Identify New Plant Signaling Peptides

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Plant Signaling Peptides

Part of the book series: Signaling and Communication in Plants ((SIGCOMM,volume 16))

Abstract

Peptides play a key role in plant development and intercellular communication. A variety of novel peptides and peptidic compounds have been isolated over the last few decades. This chapter examines the various methods used to isolate and identify new peptides and peptidic compounds from plants, and provides an overview of these methods. The principles involved in extraction and methods commonly used for separation, isolation, and structural elucidation are summarized. Methods discussed include high-performance liquid chromatography in both normal and reversed phases, ion-exchange chromatography, size-exclusion chromatography, affinity chromatography, hydrophilic interaction liquid chromatography, hydrophobic interaction chromatography, multidimensional liquid chromatography, and various physical identification techniques such as mass spectrometry for determining molecular structures. The application of such techniques to identification of novel plant peptides is stressed.

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Authors and Affiliations

  1. Computational Biosciences Research Center, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia

    Sunil Sagar & Kenneth P. Minneman

  2. Division of Chemical and Life Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Saudi Arabia

    Chris Gehring

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  1. Sunil Sagar
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Correspondence to Sunil Sagar .

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  1. , Monash Institute of Pharmaceutical Scien, Monash University, Royal Parade 381, Parkville, 3052, Australia

    Helen R. Irving

  2. , Division of Chemistry, Life Science and, 4700 King Abdullah University of Science, Thuwal, 23955-6900, Saudi Arabia

    Christoph Gehring

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Sagar, S., Gehring, C., Minneman, K.P. (2012). Methods to Isolate and Identify New Plant Signaling Peptides. In: Irving, H., Gehring, C. (eds) Plant Signaling Peptides. Signaling and Communication in Plants, vol 16. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27603-3_12

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