Hepatocyte Growth Factor (HGF)-Konzentrationen im Patientenserum sind nach partieller Hepatektomie mit dem Ausmaß an Parenchymverlust und mit postoperativ peripher mobilisierten CD34+ und CD133+ Stammzellen positiv korreliert

Abstract

In this study we investigated paracrine factors like HGF, insulin like growth factor 1 (IGF-1) stroma derived factor 1 (SDF-1) and stem cell factor (SCF) for correlation with peripheral progenitor mobilisation in the early course of hepatic resection using FACS-analyses in 30 patients and the extend of partial hepatectomy. CT-scan volumetry pre-operatively and 24 h following resection was utilized to determine the exact extend of liver volume loss. Serum growth fators and chemokine levels were compared among 20 patients with resection volume of less than 20 % (group A) of the pre-operative total liver volume – tumor volume (TLV) and 10 patients with a resection extend of 30 to 65 % (group B). In addition, peripheral CD133+ and CD34+ progenitor cells in peripheral blood were contrasted between the two groups up to post OP day (POD) 21. Early subsequent to the end of resection HGF (0, 6 and 24 h), SDF-1 (0, 3 and 6 h) and IGF-1 (3 h) were significantly increased in comparison to group A. We revealed a significant elevation from day 2 following liver surgery for CD133+ cells which was only seen for group B individuals and which was significantly superior to group A patients (p = 0.005). CD34+ cells demonstrated similar kinetics and differences among groups, however to a lesser extend. Only HGF directly correlated with mobilised mobilised CD34+ and CD133+ peripheral cells early after resection, independent of the extend of hepatectomy. These data suggest HGF to play a role in mobilisation of CD133+ and CD34+ cells respectively in regeneration processes subsequent to larger extend of loss of hepatic tissue.