Abstract
Accidental release of uranium into the environment has the potential of inducing chemical and radiological toxicity. In situ bioremediation of uranium by microbial processes has been shown to be effective for immobilizing uranium in contaminated sites. Such microbial processes are important components of biogeochemical cycles and regulate the mobility and fate of uranium in the environment. This chapter focuses on the fundamental microbial mechanisms underlying uranium immobilization with brief discussion on general considerations, approaches and challenges for uranium bioremediation.
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Acharya C, Apte SK (2013a) Insights into the interactions of cyanobacteria with uranium. Photosynth Res 118:83–94
Acharya C, Apte SK (2013b) Novel surface associated polyphosphate bodies sequester uranium in the filamentous, marine cyanobacterium, Anabaena torulosa. Metallomics 5:1595–1598
Acharya C, Joseph D, Apte SK (2009) Uranium sequestration by a marine cyanobacterium, Synechococcus elongatus strain BDU/75042. Bioresour Technol 100:2176–2181
Acharya C, Chandwadkar P, Apte SK (2012) Interaction of uranium with a filamentous, heterocystous, nitrogen-fixing cyanobacterium, Anabaena torulosa. Bioresour Technol 116:290–294
Anderson RT, Vrionis HA, Ortiz-Bernad I, Resch CT, Long PE, Dayvault R, Karp K, Marutzky S, Metzler DR, Peacock A, White DC, Lowe M, Lovley DR (2003) Stimulating the in situ activity of Geobacter species to remove uranium from the groundwater of a uranium-contaminated aquifer. Appl Environ Microbiol 69:5884–5891
Bargar JR, Williams KH, Campbell KM, Long PE, Stubbs JE, Suvorova EI, Lezama-Pacheco JS, Alessi DS, Stylo M, Webb SM, Davis JA, Giammar DE, Blue LY, Bernier-Latmani R (2013) Uranium redox transition pathways in acetate-amended sediments. Proc Natl Acad Sci U S A 110:4506–4511
Beazley MJ, Martinez RJ, Sobecky PA, Webb SM, Taillefert M (2007) Uranium biomineralization as a result of bacterial phosphatase activity: insights from bacterial isolates from a contaminated subsurface. Environ Sci Technol 41:5701–5707
Beazley MJ, Martinez RJ, Webb SM, Sobecky PA, Taillefert M (2011) The effect of pH and natural microbial phosphatase activity on the speciation of uranium in subsurface soils. Geochim Cosmochim Acta 75:5648–5663
Bernier-Latmani R, Veeramani H, Vecchia ED, Junier P, Lezama-Pacheco JS, Suvorova EI, Sharp JO, Wigginton NS, Bargar JR (2010) Non-uraninite products of microbial U(VI) reduction. Environ Sci Technol 44:9456–9462
Beveridge TJ, Fyfe WS (1985) Metal fixation by bacterial cell walls. Can J Earth Sci 22:1892–1898
Bonhoure I, Meca S, Marti V, De Pablo J, Cortina JL (2007) A new time-resolved laser-induced fluorescence spectrometry (TRLFS) data acquisition procedure applied to the uranyl-phosphate system. Radiochim Acta 95:165–172
Boonchayaanant B, Nayak D, Du X, Criddle CS (2009) Uranium reduction and resistance to reoxidation under iron-reducing and sulfate-reducing conditions. Water Res 43:4652–4664
Brendler V, Geipel G, Bernhard G, Nitsche H (1996) Complexation in the system UO2 2+/PO4 3-/OH-(aq): potentiometric and spectroscopic investigations at very low ionic strengths. Radiochim Acta 74:75–80
Brodie EL, DeSantis TZ, Joyner DC, Baek SM, Larsen JT, Andersen GL (2006) Application of a high-density oligonucleotide microarray approach to study bacterial population dynamics during uranium reduction and reoxidation. Appl Environ Microbiol 72:6288–6298
Brooks SC, Fredrickson JK, Carroll SL, Kennedy DW, Zachara JM, Plymale AE, Kelly SD, Kemner KM, Fendorf S (2003) Inhibition of bacterial U (VI) reduction by calcium. Environ Sci Technol 37:1850–1858
Cardenas E, Wu WM, Leigh MB, Carley J, Carroll S, Gentry T, Luo J, Watson D, Gu B, Ginder-Vogel M, Kitanidis PK, Jardine PM, Zhou J, Criddle CS, Marsh TL, Tiedje JA (2008) Microbial communities in contaminated sediments, associated with bioremediation of uranium to submicromolar levels. Appl Environ Microbiol 74:3718–3729
Choppin GR, Du M (1992) f-Element complexation in brine solutions. Radiochim Acta 58–9:101
Choppin T, Buschmann AH, Troell M, Kautsky N, Neori A, Yarish C (2011) Integrating seaweeds into marine aquaculture systems: a key toward sustainability. J Phycol 37:975–986
Cologgi DL, Lampa-Pastirk S, Speers AM, Kelly SD, Reguera G (2011) Extracellular reduction of uranium via Geobacter conductive pili as a protective cellular mechanism. Proc Natl Acad Sci U S A 108:15248–15252
Cotton S (2006) Coordination chemistry of the actinides. In: Cotton S (ed) Lanthanide and actinide chemistry. Wiley, Chichester, pp 173–199
DiChristina TJ (1992) Effects of nitrate and nitrite on dissimilatory iron reduction by Shewanella putrefaciens 200. J Bacteriol 174:1891–1896
Driver CJ (1994) Ecotoxicity literature review of selected Hanford Site contaminants. US Department of Energy, Pacific Northwest Laboratory, PNL-9394, Richland, p 141
Fortin C, Denison FH, Garnier-Laplace J (2007) Metal phytoplankton interactions: modelling the effect of competing ions (H+, Ca2+, and Mg 2+) on uranium uptake. Environ Toxicol Chem 26:242–248
Fowle DA, Fein JB, Martin AM (2000) Experimental study of uranyl adsorption onto Bacillus subtilis. Environ Sci Technol 34:3737–3741
Frengstad B, Kjersti A, Skrede M, Banks D, Krog JR, Siewers U (2000) The chemistry of Norwegian groundwaters: III. The distribution of trace elements in 476 crystalline bedrock groundwaters, as analysed by ICP-MS techniques. Sci Total Environ 246:21–40
Gavrilescu M, Vasile L, Cretescu I (2009) Characterization and remediation of soils contaminated with uranium. J Hazard Mater 163:475–510
Geipel G, Brachmann A, Brendler V, Bernhard G, Nitsche H (1996) Uranium (VI) sulfate complexation studied by time resolved laser-induced fluorescence spectroscopy. Radiochim Acta 75:199–204
Gihring TM, Zhang GX, Brandt CC, Brooks SC, Campbell JH, Carroll S, Criddle CS, Green SJ, Jardine P, Kostka JE, Lowe K, Mehlhorn TL, Overholt W, Watson DB, Yang ZM, Wu WM, Schadt CW (2011) A limited microbial consortium is responsible for extended bioreduction of uranium in a contaminated aquifer. Appl Environ Microbiol 77:5955–5965
Green SJ, Prakash O, Jasrotia P, Overholt WA, Cardenas E, Hubbard D, Tiedje JM, Watson DB, Schadt CW, Brooks SC, Kostka JE (2012) Denitrifying bacteria from the genus Rhodanobacter dominate bacterial communities in the highly contaminated subsurface of a nuclear legacy waste site. Appl Environ Microbiol 78:1039–1047
Guillaumont R, Fanghaenel T, Neck V, Fuger J, Palmer DA, Grenthe I, Rand MH (2003) Chemical thermodynamics 5, Update on the chemical thermodynamics of uranium, neptunium, plutonium, americium and technetium. Elsevier, Amsterdam
He Z, Gentry TJ, Schadt CW, Wu L, Liebich J, Chong SC et al (2007) GeoChip: a comprehensive microarray for investigating biogeochemical, ecological and environmental processes. ISME J 1:67–77
Hennig C, Tutschku J, Rossberg A, Bernhard G, Scheinost AC (2005) Comparative EXAFS investigation of uranium(VI) and -(IV) aquo chloro complexes in solution using a newly developed spectroelectrochemical cell. Inorg Chem 44:6655–6661
Hsi CD, Langmuir D (1985) Adsorption of uranyl onto ferric oxyhydroxides. Application of the surface complexation site-binding model. Geochim Cosmochim Acta 49:1931–1941
Hu P, Brodie EL, Suzuki Y, McAdams HH, Andersen GL (2005) Whole-genome transcriptional analysis of heavy metal stresses in Caulobacter crescentus. J Bacteriol 187:8437–8844
Istok JD, Senko JM, Krumholz LR, Watson D, Bogle MA, Peacock A, Chang YJ, White DC (2004) In situ bioreduction of technetium and uranium in a nitrate contaminated aquifer. Environ Sci Technol 38:468–475
Karpas Z, Paz-Tal O, Lorber A, Salonen L, Komulainen H, Auvinen A, Saha H, Kurttio P (2005) Urine, hair, and nails as indicators for ingestion of uranium in drinking water. Health Phys 88:229–242
Kelly SD, Kemner KM, Fein JB, Fowle DA, Boyanov MI, Bunker BA, Yee N (2002) X-ray absorption fine structure determination of pH-dependent U-bacterial cell wall interactions. Geochim Cosmochim Acta 66:3855–3871
Koban A, Geipel G, Rossberg A, Bernhard G (2004) Uranium(VI) complexes with sugar phosphates in aqueous solution. Radiochim Acta 92:903–908
Kumar R, Nongkhlaw M, Acharya C, Joshi SR (2013a) Uranium (U)-tolerant bacterial diversity from U ore deposit of Domiasiat in North-East India and its prospective utilisation in bioremediation. Microbes Environ 28:33–41
Kumar R, Nongkhlaw M, Acharya C, Joshi SR (2013b) Soil bacterial metagenomic analysis from uranium ore deposit of Domiasiat in Northeast India. Curr Sci 105:495–498
Kurttio P, Komulainen H, Leino A, Salonen L, Auvinen A, Saha H (2005) Bone as a possible target of chemical toxicity of natural uranium in drinking water. Environ Health Perspect 113:68–72
Langmuir D (1997) Aqueous geochemistry of uranium. In: McConnin R (ed) Aqueous environmental chemistry. Prentice-Hall, Upper Saddle River, NJ, pp 494–512
Lovley DR, Phillips EJP (1988) Novel mode of microbial energy metabolism: organic carbon oxidation coupled to dissimilatory reduction of iron or manganese. Appl Environ Microbiol 54:1472–1480
Lovley DR, Phillips EJP (1992) Bioremediation of uranium contamination with enzymatic uranium reduction. Environ Sci Technol 26:2228–2234
Lovley DR, Phillips EJP, Gorby YA, Landa ER (1991) Microbial reduction of uranium. Nature 350:413–416
Lovley DR, Widman PK, Woodward JC, Phillips EJP (1993) Reduction of uranium by cytochrome-c3 of Desulfovibrio vulgaris. Appl Environ Microbiol 59:3572–3576
Macaskie LE (1990) An immobilized cell bioprocess for the removal of heavy metals from aqueous flows. J Chem Technol Biotechnol 49:357–379
Macaskie LE, Empson RM, Cheetham AK, Grey CP, Skarnulis AJ (1992) Uranium bioaccumulation by a Citrobacter sp. as a result of enzymically mediated growth of polycrystalline HUO2PO4. Science 257:782–784
Madden AS, Swindle AL, Beazley MJ, Moon JW, Ravel B, Phelps TJ (2012) Long term solid-phase fate of co-precipitated U(VI)–Fe(III) following biological iron reduction by Thermoanaerobacter. Am Miner 97:1641–1652
NCRP (National Council on Radiation Protection and Measurements) (1999) Biological effects and exposure limits for “hot particles”. NCRP report no. 130, Bethesda, MD
Merroun ML, Selenska-Pobell S (2001) Interactions of three eco-types of Acidithiobacillus ferrooxidans strains with (VI). Biometals 14:171–179
Merroun ML, Raff J, Rossberg A, Hennig C, Hennig C, Reich T, Selenska-Pobell S (2005) Complexation of uranium by cells and S-layer sheets of Bacillus sphaericus JG-A12. Appl Environ Microbiol 71:5532–5543
Moll H, Reich T, Hennig C, Rossberg A, Szabo Z, Grenthe I (2000) Solution coordination chemistry of uranium in the binary UO2+–SO4 2– and the ternary UO2 2+ –SO4 2––OH– system. Radiochim Acta 88:559–565
Myers CR, Nealson KH (1988) Bacterial manganese reduction and growth with manganese oxide as the sole electron acceptor. Science 240:1319–1321
Nyman JL, Wu HI, Gentile ME, Kitanidis PK, Criddle CS (2007) Inhibition of a U(VI)-and sulfate-reducing consortia by U(VI). Environ Sci Technol 41:6528–6533
Palmer MR, Edmond JM (1993) Uranium in river water. Geochim Cosmochim Acta 57:4947–4955
Paterson-Beedle M, Jeong BC, Lee CH, Jee KY, Kim WH, Renshaw JC, Macaskie LE (2012) Radiotolerance of phosphatases of a Serratia sp.: potential for the use of this organism in the biomineralization of wastes containing radionuclides. Biotechnol Bioeng 109:1937–1946
Priest ND (2001) Toxicity of depleted uranium. Lancet 357:244–246
Reeder RJ, Nugent M, Tait CD, Morris DE, Heald SM, Beck KM, Hess WP, Lanzirotti A (2001) Coprecipitation of uranium(VI) with calcite: XAFS, micro-XAS, and luminescence characterization. Geochim Cosmoschim Acta 65:3491–3503
Renninger N, Knopp R, Nitsche H, Clark D, Keasling J (2004) Uranyl precipitation by Pseudomonas aeruginosa via controlled polyphosphate metabolism. Appl Environ Microbiol 70:7404–7412
Ruggiero CE, Boukhalfa H, Forsythe JH, Lack JG, Hersman LE, Neu MP (2005) Actinide and metal toxicity to prospective bioremediation bacteria. Environ Microbiol 7:88–97
Sandino A, Bruno J (1992) The solubility of (UO2)3(PO4)2⋅4H2O(S) and the formation of U (VI) phosphate complexes: their influence speciation in natural waters. Geochim Cosmochim Acta 56:4135–4145
Scapolan S, Ansoborlo E, Moulin C, Madic C (1998) Uranium (VI)–transferrin system studied by time resolved laser-induced fluorescence. Radiat Prot Dosim 79:505–508
Schiewer S, Volesky B (2000) Biosorption by marine algae. In: Valdes JJ (ed) Remediation. Kluwer, Dordrecht, pp 139–169
Senko JM, Kelly SD, Dohnalkova AC, McDonough JT, Kemner KM, Burgos WD (2007) The effect of U(VI) bioreduction kinetics on subsequent reoxidation of biogenic U(IV). Geochim Cosmochim Acta 71:4644–4654
Suzuki Y, Banfield JF (1999) Geomicrobiology of uranium. In: Redfern SAT, Carpenter MA (eds) Transformation processes in minerals, Reviews in mineralogy and geochemistry. Mineralogical Society of America, Washington, DC, pp 393–432
Suzuki Y, Banfield JF (2004) Resistance to, and accumulation of, uranium by bacteria from a uranium-contaminated site. J Geomicrobiol 21:113–121
Thomas RAP, Macaskie LE (1996) Biodegradation of tributyl phosphate by naturally occurring microbial isolates and coupling to the removal of uranium from aqueous solution. Environ Sci Technol 30:2371–2375
VanEngelen MR, Field EK, Gerlach R, Lee BD, Apel WA, Peyton BM (2010) UO2 2+ speciation determines uranium toxicity and bioaccumulation in an environmental Pseudomonas sp. isolate. Environ Toxicol Chem 29:763–769
Van Groenestijn JW, Vlekke GJFM, Anink DME, Deinema MH, Zehnder AJB (1988) Role of cations in accumulation and release of phosphate by Acinetobacter strain 210A. Appl Environ Microbiol 54:2894–2901
Van Roy S, Peys K, Dresselaers T, Diels L (1997) The use of an Alcaligenes eutrophus biofilm in a membrane bioreactor for heavy metals recovery. Res Microbiol 148:526–528
Wade R, DiChristina TJ (2000) Isolation of U (VI) reduction-deficient mutants of Shewanella putrefaciens. FEMS Microbiol Lett 184:143–148
Williams KH, Long PE, Davis JA, Wilkins MJ, N’Guessan AL, Steefel CI, Yang L, Newcomer D, Spane FA, Kerkhof LJ, McGuinness L, Dayvault R, Lovley DR (2011) Acetate availability and its influence on sustainable bioremediation of uranium-contaminated groundwater. Geomicrobiol J 28:519–539
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Acharya, C. (2015). Uranium Bioremediation: Approaches and Challenges. In: Sukla, L., Pradhan, N., Panda, S., Mishra, B. (eds) Environmental Microbial Biotechnology. Soil Biology, vol 45. Springer, Cham. https://doi.org/10.1007/978-3-319-19018-1_7
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DOI: https://doi.org/10.1007/978-3-319-19018-1_7
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