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Scaling-Up Recombinant Enzyme Fermentation

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Recombinant Enzymes - From Basic Science to Commercialization

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Abstract

Bioreactor or fermenter is the heart of a process. Scaling up a bioreactor and maintaining the same optimized process in a recombinant enzyme production in a higher scale is an engineering challenge. Scale-up is about mixing and mass transfer, a combination of art and science. It is more to methodological approach with some combination of rules of thumb, experience and/or trial and error. This chapter presented several bioreactor or fermenter modes and types before encompasses four different scale-up strategies. The scale-up strategies related to mass transfer involving constants scale-up of; (i)volumetric transfer coefficient (KLa), (ii) power input per liquid volume (P/V), (iii) impeller tip speed (Vi), and (iv) mixing time (t m) are described and discussed.

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Authors and Affiliations

  1. Biomolecular and Bioprocess Engineering Research Unit, Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, P.O. Box 10, 50728, Kuala Lumpur, Malaysia

    Azlin Suhaida Azmi, Nor Fadhillah Mohamed Amin & Fathilah Ali

  2. Department of Biotechnology Engineering, Bioenvironmental Engineering Research Centre, Faculty of Engineering, International Islamic University Malaysia, P. O. Box 10, 50728, Kuala Lumpur, Malaysia

    Sarina Sulaiman

Authors
  1. Azlin Suhaida Azmi
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  2. Sarina Sulaiman
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  3. Nor Fadhillah Mohamed Amin
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  4. Fathilah Ali
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Correspondence to Azlin Suhaida Azmi .

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Editors and Affiliations

  1. Department of Biotechnology Engineering Kulliyyah of Engineering, International Islamic University Malaysia, Kuala Lumpur, Malaysia

    Azura Amid

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Azmi, A., Sulaiman, S., Amin, N., Ali, F. (2015). Scaling-Up Recombinant Enzyme Fermentation. In: Amid, A. (eds) Recombinant Enzymes - From Basic Science to Commercialization. Springer, Cham. https://doi.org/10.1007/978-3-319-12397-4_7

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